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The Effect of Lycopene Preexposure on UV-B-Irradiated Human Keratinocytes.

Ascenso A, Pedrosa T, Pinho S, Pinho F, de Oliveira JM, Cabral Marques H, Oliveira H, Simões S, Santos C - Oxid Med Cell Longev (2015)

Bottom Line: Lycopene did not significantly affect the profile of apoptotic, necrotic and viable cells in nonirradiated cells neither showed cytostatic effects.In irradiated cells, lycopene preexposure resulted in overexpression of BAX gene compared to nonexposed irradiated cells.This was accompanied by a cell cycle delay at S-phase transition and consequent decrease of cells in G0/G1 phase.

View Article: PubMed Central - PubMed

Affiliation: Instituto de Investigação do Medicamento (iMed.ULisboa), Faculdade de Farmácia, Universidade de Lisboa, Avenida Professor Gama Pinto, 1649-003 Lisboa, Portugal ; Departamento de Biologia, Laboratório de Biotecnologia e Citómica, CESAM, Universidade de Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal.

ABSTRACT

Unlabelled: Lycopene has been reported as the antioxidant most quickly depleted in skin upon UV irradiation, and thus it might play a protective role. Our goal was to investigate the effects of preexposure to lycopene on UV-B-irradiated skin cells. Cells were exposed for 24 h to 10 M lycopene, and subsequently irradiated and left to recover for another 24 h period. Thereafter, several parameters were analyzed by FCM and

Rt-pcr: genotoxicity/clastogenicity by assessing the cell cycle distribution; apoptosis by performing the Annexin-V assay and analyzing gene expression of apoptosis biomarkers; and oxidative stress by ROS quantification. Lycopene did not significantly affect the profile of apoptotic, necrotic and viable cells in nonirradiated cells neither showed cytostatic effects. However, irradiated cells previously treated with lycopene showed an increase in both dead and viable subpopulations compared to nonexposed irradiated cells. In irradiated cells, lycopene preexposure resulted in overexpression of BAX gene compared to nonexposed irradiated cells. This was accompanied by a cell cycle delay at S-phase transition and consequent decrease of cells in G0/G1 phase. Thus, lycopene seems to play a corrective role in irradiated cells depending on the level of photodamage. Thus, our findings may have implications for the management of skin cancer.

No MeSH data available.


Related in: MedlinePlus

Effect of complexed lycopene preexposure on UV-B-irradiated (IR, 225 mJ/cm2) and nonirradiated (NI) HaCaT cells on cell MA measured by MTT assay. Results are expressed as percentage (mean ± SD of 3 independent experiments with 6 replicates each one). Statistical analysis: One-Way ANOVA with Multiple Comparisons versus Control Group (Holm-Sidak method): statistical differences between the samples within nonirradiated and irradiated groups (in respect to cells not exposed to lycopene) are represented by different letters when P < 0.05.
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Related In: Results  -  Collection


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fig4: Effect of complexed lycopene preexposure on UV-B-irradiated (IR, 225 mJ/cm2) and nonirradiated (NI) HaCaT cells on cell MA measured by MTT assay. Results are expressed as percentage (mean ± SD of 3 independent experiments with 6 replicates each one). Statistical analysis: One-Way ANOVA with Multiple Comparisons versus Control Group (Holm-Sidak method): statistical differences between the samples within nonirradiated and irradiated groups (in respect to cells not exposed to lycopene) are represented by different letters when P < 0.05.

Mentions: Complexed lycopene (Lyc-CD) up to 15 μM did not affect the metabolic activity of nonirradiated cells, and only 20 μM Lyc-CD led to significant decrease in metabolic activity in these cells (Figure 4). At doses equal to or higher than 15 μM, complexed lycopene decreases the MA of irradiated cells (225 mJ/cm2), compared to cells not preexposed to lycopene (Figure 4).


The Effect of Lycopene Preexposure on UV-B-Irradiated Human Keratinocytes.

Ascenso A, Pedrosa T, Pinho S, Pinho F, de Oliveira JM, Cabral Marques H, Oliveira H, Simões S, Santos C - Oxid Med Cell Longev (2015)

Effect of complexed lycopene preexposure on UV-B-irradiated (IR, 225 mJ/cm2) and nonirradiated (NI) HaCaT cells on cell MA measured by MTT assay. Results are expressed as percentage (mean ± SD of 3 independent experiments with 6 replicates each one). Statistical analysis: One-Way ANOVA with Multiple Comparisons versus Control Group (Holm-Sidak method): statistical differences between the samples within nonirradiated and irradiated groups (in respect to cells not exposed to lycopene) are represented by different letters when P < 0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4664803&req=5

fig4: Effect of complexed lycopene preexposure on UV-B-irradiated (IR, 225 mJ/cm2) and nonirradiated (NI) HaCaT cells on cell MA measured by MTT assay. Results are expressed as percentage (mean ± SD of 3 independent experiments with 6 replicates each one). Statistical analysis: One-Way ANOVA with Multiple Comparisons versus Control Group (Holm-Sidak method): statistical differences between the samples within nonirradiated and irradiated groups (in respect to cells not exposed to lycopene) are represented by different letters when P < 0.05.
Mentions: Complexed lycopene (Lyc-CD) up to 15 μM did not affect the metabolic activity of nonirradiated cells, and only 20 μM Lyc-CD led to significant decrease in metabolic activity in these cells (Figure 4). At doses equal to or higher than 15 μM, complexed lycopene decreases the MA of irradiated cells (225 mJ/cm2), compared to cells not preexposed to lycopene (Figure 4).

Bottom Line: Lycopene did not significantly affect the profile of apoptotic, necrotic and viable cells in nonirradiated cells neither showed cytostatic effects.In irradiated cells, lycopene preexposure resulted in overexpression of BAX gene compared to nonexposed irradiated cells.This was accompanied by a cell cycle delay at S-phase transition and consequent decrease of cells in G0/G1 phase.

View Article: PubMed Central - PubMed

Affiliation: Instituto de Investigação do Medicamento (iMed.ULisboa), Faculdade de Farmácia, Universidade de Lisboa, Avenida Professor Gama Pinto, 1649-003 Lisboa, Portugal ; Departamento de Biologia, Laboratório de Biotecnologia e Citómica, CESAM, Universidade de Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal.

ABSTRACT

Unlabelled: Lycopene has been reported as the antioxidant most quickly depleted in skin upon UV irradiation, and thus it might play a protective role. Our goal was to investigate the effects of preexposure to lycopene on UV-B-irradiated skin cells. Cells were exposed for 24 h to 10 M lycopene, and subsequently irradiated and left to recover for another 24 h period. Thereafter, several parameters were analyzed by FCM and

Rt-pcr: genotoxicity/clastogenicity by assessing the cell cycle distribution; apoptosis by performing the Annexin-V assay and analyzing gene expression of apoptosis biomarkers; and oxidative stress by ROS quantification. Lycopene did not significantly affect the profile of apoptotic, necrotic and viable cells in nonirradiated cells neither showed cytostatic effects. However, irradiated cells previously treated with lycopene showed an increase in both dead and viable subpopulations compared to nonexposed irradiated cells. In irradiated cells, lycopene preexposure resulted in overexpression of BAX gene compared to nonexposed irradiated cells. This was accompanied by a cell cycle delay at S-phase transition and consequent decrease of cells in G0/G1 phase. Thus, lycopene seems to play a corrective role in irradiated cells depending on the level of photodamage. Thus, our findings may have implications for the management of skin cancer.

No MeSH data available.


Related in: MedlinePlus