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The Effect of Lycopene Preexposure on UV-B-Irradiated Human Keratinocytes.

Ascenso A, Pedrosa T, Pinho S, Pinho F, de Oliveira JM, Cabral Marques H, Oliveira H, Simões S, Santos C - Oxid Med Cell Longev (2015)

Bottom Line: Lycopene did not significantly affect the profile of apoptotic, necrotic and viable cells in nonirradiated cells neither showed cytostatic effects.In irradiated cells, lycopene preexposure resulted in overexpression of BAX gene compared to nonexposed irradiated cells.This was accompanied by a cell cycle delay at S-phase transition and consequent decrease of cells in G0/G1 phase.

View Article: PubMed Central - PubMed

Affiliation: Instituto de Investigação do Medicamento (iMed.ULisboa), Faculdade de Farmácia, Universidade de Lisboa, Avenida Professor Gama Pinto, 1649-003 Lisboa, Portugal ; Departamento de Biologia, Laboratório de Biotecnologia e Citómica, CESAM, Universidade de Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal.

ABSTRACT

Unlabelled: Lycopene has been reported as the antioxidant most quickly depleted in skin upon UV irradiation, and thus it might play a protective role. Our goal was to investigate the effects of preexposure to lycopene on UV-B-irradiated skin cells. Cells were exposed for 24 h to 10 M lycopene, and subsequently irradiated and left to recover for another 24 h period. Thereafter, several parameters were analyzed by FCM and

Rt-pcr: genotoxicity/clastogenicity by assessing the cell cycle distribution; apoptosis by performing the Annexin-V assay and analyzing gene expression of apoptosis biomarkers; and oxidative stress by ROS quantification. Lycopene did not significantly affect the profile of apoptotic, necrotic and viable cells in nonirradiated cells neither showed cytostatic effects. However, irradiated cells previously treated with lycopene showed an increase in both dead and viable subpopulations compared to nonexposed irradiated cells. In irradiated cells, lycopene preexposure resulted in overexpression of BAX gene compared to nonexposed irradiated cells. This was accompanied by a cell cycle delay at S-phase transition and consequent decrease of cells in G0/G1 phase. Thus, lycopene seems to play a corrective role in irradiated cells depending on the level of photodamage. Thus, our findings may have implications for the management of skin cancer.

No MeSH data available.


Related in: MedlinePlus

(a) HaCaT cells growth and confluence curves under normal culture conditions for 120 h. (b) HaCaT cells observed by phase-contrast microscope (100x magnification), scale bar: 20 μm.
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fig2: (a) HaCaT cells growth and confluence curves under normal culture conditions for 120 h. (b) HaCaT cells observed by phase-contrast microscope (100x magnification), scale bar: 20 μm.

Mentions: HaCaT cell growth and confluence under normal culture conditions until 120 h are represented on Figure 2(a). As it can be observed, the exponential phase extends until approximately 72 h and the full confluence can be maintained more than 1 week. The selected confluence for complexed lycopene exposure and UV irradiation in this experiment was attained at 24 h and 48 h, respectively.


The Effect of Lycopene Preexposure on UV-B-Irradiated Human Keratinocytes.

Ascenso A, Pedrosa T, Pinho S, Pinho F, de Oliveira JM, Cabral Marques H, Oliveira H, Simões S, Santos C - Oxid Med Cell Longev (2015)

(a) HaCaT cells growth and confluence curves under normal culture conditions for 120 h. (b) HaCaT cells observed by phase-contrast microscope (100x magnification), scale bar: 20 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4664803&req=5

fig2: (a) HaCaT cells growth and confluence curves under normal culture conditions for 120 h. (b) HaCaT cells observed by phase-contrast microscope (100x magnification), scale bar: 20 μm.
Mentions: HaCaT cell growth and confluence under normal culture conditions until 120 h are represented on Figure 2(a). As it can be observed, the exponential phase extends until approximately 72 h and the full confluence can be maintained more than 1 week. The selected confluence for complexed lycopene exposure and UV irradiation in this experiment was attained at 24 h and 48 h, respectively.

Bottom Line: Lycopene did not significantly affect the profile of apoptotic, necrotic and viable cells in nonirradiated cells neither showed cytostatic effects.In irradiated cells, lycopene preexposure resulted in overexpression of BAX gene compared to nonexposed irradiated cells.This was accompanied by a cell cycle delay at S-phase transition and consequent decrease of cells in G0/G1 phase.

View Article: PubMed Central - PubMed

Affiliation: Instituto de Investigação do Medicamento (iMed.ULisboa), Faculdade de Farmácia, Universidade de Lisboa, Avenida Professor Gama Pinto, 1649-003 Lisboa, Portugal ; Departamento de Biologia, Laboratório de Biotecnologia e Citómica, CESAM, Universidade de Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal.

ABSTRACT

Unlabelled: Lycopene has been reported as the antioxidant most quickly depleted in skin upon UV irradiation, and thus it might play a protective role. Our goal was to investigate the effects of preexposure to lycopene on UV-B-irradiated skin cells. Cells were exposed for 24 h to 10 M lycopene, and subsequently irradiated and left to recover for another 24 h period. Thereafter, several parameters were analyzed by FCM and

Rt-pcr: genotoxicity/clastogenicity by assessing the cell cycle distribution; apoptosis by performing the Annexin-V assay and analyzing gene expression of apoptosis biomarkers; and oxidative stress by ROS quantification. Lycopene did not significantly affect the profile of apoptotic, necrotic and viable cells in nonirradiated cells neither showed cytostatic effects. However, irradiated cells previously treated with lycopene showed an increase in both dead and viable subpopulations compared to nonexposed irradiated cells. In irradiated cells, lycopene preexposure resulted in overexpression of BAX gene compared to nonexposed irradiated cells. This was accompanied by a cell cycle delay at S-phase transition and consequent decrease of cells in G0/G1 phase. Thus, lycopene seems to play a corrective role in irradiated cells depending on the level of photodamage. Thus, our findings may have implications for the management of skin cancer.

No MeSH data available.


Related in: MedlinePlus