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The Effect of Lycopene Preexposure on UV-B-Irradiated Human Keratinocytes.

Ascenso A, Pedrosa T, Pinho S, Pinho F, de Oliveira JM, Cabral Marques H, Oliveira H, Simões S, Santos C - Oxid Med Cell Longev (2015)

Bottom Line: Lycopene did not significantly affect the profile of apoptotic, necrotic and viable cells in nonirradiated cells neither showed cytostatic effects.In irradiated cells, lycopene preexposure resulted in overexpression of BAX gene compared to nonexposed irradiated cells.This was accompanied by a cell cycle delay at S-phase transition and consequent decrease of cells in G0/G1 phase.

View Article: PubMed Central - PubMed

Affiliation: Instituto de Investigação do Medicamento (iMed.ULisboa), Faculdade de Farmácia, Universidade de Lisboa, Avenida Professor Gama Pinto, 1649-003 Lisboa, Portugal ; Departamento de Biologia, Laboratório de Biotecnologia e Citómica, CESAM, Universidade de Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal.

ABSTRACT

Unlabelled: Lycopene has been reported as the antioxidant most quickly depleted in skin upon UV irradiation, and thus it might play a protective role. Our goal was to investigate the effects of preexposure to lycopene on UV-B-irradiated skin cells. Cells were exposed for 24 h to 10 M lycopene, and subsequently irradiated and left to recover for another 24 h period. Thereafter, several parameters were analyzed by FCM and

Rt-pcr: genotoxicity/clastogenicity by assessing the cell cycle distribution; apoptosis by performing the Annexin-V assay and analyzing gene expression of apoptosis biomarkers; and oxidative stress by ROS quantification. Lycopene did not significantly affect the profile of apoptotic, necrotic and viable cells in nonirradiated cells neither showed cytostatic effects. However, irradiated cells previously treated with lycopene showed an increase in both dead and viable subpopulations compared to nonexposed irradiated cells. In irradiated cells, lycopene preexposure resulted in overexpression of BAX gene compared to nonexposed irradiated cells. This was accompanied by a cell cycle delay at S-phase transition and consequent decrease of cells in G0/G1 phase. Thus, lycopene seems to play a corrective role in irradiated cells depending on the level of photodamage. Thus, our findings may have implications for the management of skin cancer.

No MeSH data available.


Related in: MedlinePlus

Culture conditions and experimental setting for the study of HaCaT cells exposed to complexed lycopene (Lyc-CD) and UV-B irradiation.
© Copyright Policy - open-access
Related In: Results  -  Collection


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fig1: Culture conditions and experimental setting for the study of HaCaT cells exposed to complexed lycopene (Lyc-CD) and UV-B irradiation.

Mentions: From previous cell viability results of HaCaT cells exposed to UV-B irradiation, new exposure conditions were tested with different Lyc-CD concentrations and a fixed UV-B irradiation dose. Briefly, cells were seeded and grown in DMEM (Dulbecco's modified Eagle's medium) supplemented with 10% FBS, 1% penicillin-streptomycin, and 1% fungizone, for 24 h, and then DMEM medium was replaced with α-MEM without nucleosides (Gibco, Life Technologies) with identical supplementation, containing complexed lycopene solutions (0, 5, 10, 15, and 20 μM). Cells were exposed for 24 h, and after UV-B irradiation (225 mJ/cm2) HaCaT irradiated cells were incubated under standard culture conditions for a final 24 h period. The metabolic activity of cell culture was analyzed by MTT assay in order to choose just one lycopene dose for the subsequent analyses (Figure 1).


The Effect of Lycopene Preexposure on UV-B-Irradiated Human Keratinocytes.

Ascenso A, Pedrosa T, Pinho S, Pinho F, de Oliveira JM, Cabral Marques H, Oliveira H, Simões S, Santos C - Oxid Med Cell Longev (2015)

Culture conditions and experimental setting for the study of HaCaT cells exposed to complexed lycopene (Lyc-CD) and UV-B irradiation.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4664803&req=5

fig1: Culture conditions and experimental setting for the study of HaCaT cells exposed to complexed lycopene (Lyc-CD) and UV-B irradiation.
Mentions: From previous cell viability results of HaCaT cells exposed to UV-B irradiation, new exposure conditions were tested with different Lyc-CD concentrations and a fixed UV-B irradiation dose. Briefly, cells were seeded and grown in DMEM (Dulbecco's modified Eagle's medium) supplemented with 10% FBS, 1% penicillin-streptomycin, and 1% fungizone, for 24 h, and then DMEM medium was replaced with α-MEM without nucleosides (Gibco, Life Technologies) with identical supplementation, containing complexed lycopene solutions (0, 5, 10, 15, and 20 μM). Cells were exposed for 24 h, and after UV-B irradiation (225 mJ/cm2) HaCaT irradiated cells were incubated under standard culture conditions for a final 24 h period. The metabolic activity of cell culture was analyzed by MTT assay in order to choose just one lycopene dose for the subsequent analyses (Figure 1).

Bottom Line: Lycopene did not significantly affect the profile of apoptotic, necrotic and viable cells in nonirradiated cells neither showed cytostatic effects.In irradiated cells, lycopene preexposure resulted in overexpression of BAX gene compared to nonexposed irradiated cells.This was accompanied by a cell cycle delay at S-phase transition and consequent decrease of cells in G0/G1 phase.

View Article: PubMed Central - PubMed

Affiliation: Instituto de Investigação do Medicamento (iMed.ULisboa), Faculdade de Farmácia, Universidade de Lisboa, Avenida Professor Gama Pinto, 1649-003 Lisboa, Portugal ; Departamento de Biologia, Laboratório de Biotecnologia e Citómica, CESAM, Universidade de Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal.

ABSTRACT

Unlabelled: Lycopene has been reported as the antioxidant most quickly depleted in skin upon UV irradiation, and thus it might play a protective role. Our goal was to investigate the effects of preexposure to lycopene on UV-B-irradiated skin cells. Cells were exposed for 24 h to 10 M lycopene, and subsequently irradiated and left to recover for another 24 h period. Thereafter, several parameters were analyzed by FCM and

Rt-pcr: genotoxicity/clastogenicity by assessing the cell cycle distribution; apoptosis by performing the Annexin-V assay and analyzing gene expression of apoptosis biomarkers; and oxidative stress by ROS quantification. Lycopene did not significantly affect the profile of apoptotic, necrotic and viable cells in nonirradiated cells neither showed cytostatic effects. However, irradiated cells previously treated with lycopene showed an increase in both dead and viable subpopulations compared to nonexposed irradiated cells. In irradiated cells, lycopene preexposure resulted in overexpression of BAX gene compared to nonexposed irradiated cells. This was accompanied by a cell cycle delay at S-phase transition and consequent decrease of cells in G0/G1 phase. Thus, lycopene seems to play a corrective role in irradiated cells depending on the level of photodamage. Thus, our findings may have implications for the management of skin cancer.

No MeSH data available.


Related in: MedlinePlus