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The Chinese Herb Jianpijiedu Contributes to the Regulation of OATP1B2 and ABCC2 in a Rat Model of Orthotopic Transplantation Liver Cancer Pretreated with Food Restriction and Diarrhea.

Sun B, Chen Y, Xiang T, Zhang L, Chen Z, Zhang S, Zhou H, Chen S - Biomed Res Int (2015)

Bottom Line: Traditional Chinese Medicine Jianpijiedu decoction (JPJD) could improve the general status of liver cancer patients in clinics, especially the symptoms of decreased food intake and diarrhea.It was also shown that the high dose of JPJD significantly improved the survival rate, weight, and organ weight when compared with FRD-LC-induced rats.Moreover, JPJD administration upregulated the mRNA and protein levels of ABCC2 and downregulated the mRNA and protein levels of OATP1B2 in liver tissues.

View Article: PubMed Central - PubMed

Affiliation: Department of Traditional Chinese Medicine, The First Affiliated Hospital, Sun Yat-sen University, 58 Zhongshan Road II, Guangzhou 510080, China.

ABSTRACT
Traditional Chinese Medicine Jianpijiedu decoction (JPJD) could improve the general status of liver cancer patients in clinics, especially the symptoms of decreased food intake and diarrhea. In this study, our results showed that the survival rate of the liver cancer with food restriction and diarrhea (FRD-LC) rats was lower than the liver cancer (LC) rats, and the tumor volume of the FRD-LC rats was higher than the LC rats. It was also shown that the high dose of JPJD significantly improved the survival rate, weight, and organ weight when compared with FRD-LC-induced rats. Moreover, JPJD administration upregulated the mRNA and protein levels of ABCC2 and downregulated the mRNA and protein levels of OATP1B2 in liver tissues. However, opposite results were observed in the cancer tissues. In conclusion, the study indicated that the Chinese Medicine JPJD could contribute to the rats with liver cancer which were pretreated with food restriction and diarrhea by regulating the expression of ABCC2 and OATP1B2 in liver tissues and cancer tissues.

No MeSH data available.


Related in: MedlinePlus

OATP1B2 and ABCC2 expression in liver and tumor tissue. (a) OATP1B2 expression in the liver cytoplasm and cell membrane (40x), (b) OATP1B2 expression in the tumor cytoplasm (40x), (c) ABCC2 expression in the liver cytoplasm (40x), and (d) ABCC2 expression in the tumor cytoplasm (40x).
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fig6: OATP1B2 and ABCC2 expression in liver and tumor tissue. (a) OATP1B2 expression in the liver cytoplasm and cell membrane (40x), (b) OATP1B2 expression in the tumor cytoplasm (40x), (c) ABCC2 expression in the liver cytoplasm (40x), and (d) ABCC2 expression in the tumor cytoplasm (40x).

Mentions: OATP1B2 was highly expressed in the liver cell membrane of the liver tissue of the normal rats (Group A) (Figure 6(a)) and in the tumor tissue of the LC-induced rats (Group B). OATP1B2 was also expressed in the cytoplasm (Figure 6(b)). ABCC2 was expressed in the cytoplasm of the liver cells of the normal rats (Group A) with stronger expression in the cells closer to the central hepatic lobule compared with the cells farther away from the central vein (Figure 6(c)). ABCC2 was expressed in low level in liver tumor tissues (Figure 6(d)).


The Chinese Herb Jianpijiedu Contributes to the Regulation of OATP1B2 and ABCC2 in a Rat Model of Orthotopic Transplantation Liver Cancer Pretreated with Food Restriction and Diarrhea.

Sun B, Chen Y, Xiang T, Zhang L, Chen Z, Zhang S, Zhou H, Chen S - Biomed Res Int (2015)

OATP1B2 and ABCC2 expression in liver and tumor tissue. (a) OATP1B2 expression in the liver cytoplasm and cell membrane (40x), (b) OATP1B2 expression in the tumor cytoplasm (40x), (c) ABCC2 expression in the liver cytoplasm (40x), and (d) ABCC2 expression in the tumor cytoplasm (40x).
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4664790&req=5

fig6: OATP1B2 and ABCC2 expression in liver and tumor tissue. (a) OATP1B2 expression in the liver cytoplasm and cell membrane (40x), (b) OATP1B2 expression in the tumor cytoplasm (40x), (c) ABCC2 expression in the liver cytoplasm (40x), and (d) ABCC2 expression in the tumor cytoplasm (40x).
Mentions: OATP1B2 was highly expressed in the liver cell membrane of the liver tissue of the normal rats (Group A) (Figure 6(a)) and in the tumor tissue of the LC-induced rats (Group B). OATP1B2 was also expressed in the cytoplasm (Figure 6(b)). ABCC2 was expressed in the cytoplasm of the liver cells of the normal rats (Group A) with stronger expression in the cells closer to the central hepatic lobule compared with the cells farther away from the central vein (Figure 6(c)). ABCC2 was expressed in low level in liver tumor tissues (Figure 6(d)).

Bottom Line: Traditional Chinese Medicine Jianpijiedu decoction (JPJD) could improve the general status of liver cancer patients in clinics, especially the symptoms of decreased food intake and diarrhea.It was also shown that the high dose of JPJD significantly improved the survival rate, weight, and organ weight when compared with FRD-LC-induced rats.Moreover, JPJD administration upregulated the mRNA and protein levels of ABCC2 and downregulated the mRNA and protein levels of OATP1B2 in liver tissues.

View Article: PubMed Central - PubMed

Affiliation: Department of Traditional Chinese Medicine, The First Affiliated Hospital, Sun Yat-sen University, 58 Zhongshan Road II, Guangzhou 510080, China.

ABSTRACT
Traditional Chinese Medicine Jianpijiedu decoction (JPJD) could improve the general status of liver cancer patients in clinics, especially the symptoms of decreased food intake and diarrhea. In this study, our results showed that the survival rate of the liver cancer with food restriction and diarrhea (FRD-LC) rats was lower than the liver cancer (LC) rats, and the tumor volume of the FRD-LC rats was higher than the LC rats. It was also shown that the high dose of JPJD significantly improved the survival rate, weight, and organ weight when compared with FRD-LC-induced rats. Moreover, JPJD administration upregulated the mRNA and protein levels of ABCC2 and downregulated the mRNA and protein levels of OATP1B2 in liver tissues. However, opposite results were observed in the cancer tissues. In conclusion, the study indicated that the Chinese Medicine JPJD could contribute to the rats with liver cancer which were pretreated with food restriction and diarrhea by regulating the expression of ABCC2 and OATP1B2 in liver tissues and cancer tissues.

No MeSH data available.


Related in: MedlinePlus