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Multi-omic profiling of MYCN-amplified neuroblastoma cell-lines.

Dassi E, Greco V, Sidarovich V, Zuccotti P, Arseni N, Scaruffi P, Tonini GP, Quattrone A - Genom Data (2015)

Bottom Line: Its most aggressive subtype, characterized by the amplification of the MYCN oncogene, has a dismal prognosis and no effective treatment is available.Understanding the alterations induced by the tumor on the various layers of gene expression is therefore important for a complete characterization of this neuroblastoma subtype and for the discovery of new therapeutic opportunities.We provide detailed experimental and data analysis procedures by means of which we derived the results described in [1].

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Translational Genomics, Centre for Integrative Biology, University of Trento, Italy.

ABSTRACT
Neuroblastoma is the most common pediatric cancer, arising from the neural crest cells of the sympathetic nervous system. Its most aggressive subtype, characterized by the amplification of the MYCN oncogene, has a dismal prognosis and no effective treatment is available. Understanding the alterations induced by the tumor on the various layers of gene expression is therefore important for a complete characterization of this neuroblastoma subtype and for the discovery of new therapeutic opportunities. Here we describe the profiling of 13 MYCN-amplified neuroblastoma cell lines at the genome (copy number), transcriptome, translatome and miRome levels (GEO series GSE56654, GSE56552 and GSE56655). We provide detailed experimental and data analysis procedures by means of which we derived the results described in [1].

No MeSH data available.


Related in: MedlinePlus

Experimental design. List the cell lines employed in the omic profiling at the various levels (genome, transcriptome, translatome and miRome) included in this study.
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f0005: Experimental design. List the cell lines employed in the omic profiling at the various levels (genome, transcriptome, translatome and miRome) included in this study.

Mentions: We performed one copy number (aCGH), transcriptome (total RNA), translatome (RNA associated to polysomes) and miRome (microRNA expression) profiling for each cell line, thus obtaining 13 samples for each profiled level (except for the miRome, consisting of 11 samples) [1]. The experimental design is also depicted in Fig. 1.


Multi-omic profiling of MYCN-amplified neuroblastoma cell-lines.

Dassi E, Greco V, Sidarovich V, Zuccotti P, Arseni N, Scaruffi P, Tonini GP, Quattrone A - Genom Data (2015)

Experimental design. List the cell lines employed in the omic profiling at the various levels (genome, transcriptome, translatome and miRome) included in this study.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4664780&req=5

f0005: Experimental design. List the cell lines employed in the omic profiling at the various levels (genome, transcriptome, translatome and miRome) included in this study.
Mentions: We performed one copy number (aCGH), transcriptome (total RNA), translatome (RNA associated to polysomes) and miRome (microRNA expression) profiling for each cell line, thus obtaining 13 samples for each profiled level (except for the miRome, consisting of 11 samples) [1]. The experimental design is also depicted in Fig. 1.

Bottom Line: Its most aggressive subtype, characterized by the amplification of the MYCN oncogene, has a dismal prognosis and no effective treatment is available.Understanding the alterations induced by the tumor on the various layers of gene expression is therefore important for a complete characterization of this neuroblastoma subtype and for the discovery of new therapeutic opportunities.We provide detailed experimental and data analysis procedures by means of which we derived the results described in [1].

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Translational Genomics, Centre for Integrative Biology, University of Trento, Italy.

ABSTRACT
Neuroblastoma is the most common pediatric cancer, arising from the neural crest cells of the sympathetic nervous system. Its most aggressive subtype, characterized by the amplification of the MYCN oncogene, has a dismal prognosis and no effective treatment is available. Understanding the alterations induced by the tumor on the various layers of gene expression is therefore important for a complete characterization of this neuroblastoma subtype and for the discovery of new therapeutic opportunities. Here we describe the profiling of 13 MYCN-amplified neuroblastoma cell lines at the genome (copy number), transcriptome, translatome and miRome levels (GEO series GSE56654, GSE56552 and GSE56655). We provide detailed experimental and data analysis procedures by means of which we derived the results described in [1].

No MeSH data available.


Related in: MedlinePlus