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Early changes in shoot transcriptome of rice in response to Rhodotorula mucilaginosa JGTA-S1.

Saha C, Seal A - Genom Data (2015)

Bottom Line: Some of the Rhodotorula species are found to exhibit plant growth promoting activities and also have been reported to protect plants against invading pathogens.Transcriptional changes were monitored in 6 h and 24 h treated rice plant shoots as compared to 0 h control.The microarray data has been submitted to the NCBI GEO repository under the accession number of GSE64321.

View Article: PubMed Central - PubMed

Affiliation: Department of Biotechnology, Dr. B.C. Guha Centre for Genetic Engineering and Biotechnology, University of Calcutta, 35, Ballygunge Circular Road, Kolkata700019, West Bengal, India.

ABSTRACT
Yeasts of Rhodotorula genus have been reported to show endophytic colonization in different plants. Some of the Rhodotorula species are found to exhibit plant growth promoting activities and also have been reported to protect plants against invading pathogens. A yeast strain closely related to Rhodotorula mucilaginosa was isolated from the endosphere of Typha angustifolia collected from a Uranium mine. A microarray analysis was performed to investigate the early changes in rice shoot transcripts in response to this yeast (R. mucilaginosa JGTA-S1). Transcriptional changes were monitored in 6 h and 24 h treated rice plant shoots as compared to 0 h control. The microarray data has been submitted to the NCBI GEO repository under the accession number of GSE64321.

No MeSH data available.


Related in: MedlinePlus

Validation of the microarray data with chosen genes: cDNA was prepared from total RNA isolated from shoots of control (0 h), 6 h, 24 h R. mucilaginosa treated rice plants. BAK1 = Brassinosteroid insensitive 1-associated receptor kinase 1 precursor (LOC_Os11g31540), GDSL like lipase (LOC_Os02g57110), GSGOGAT = glutamate synthase (LOC_Os05g48200.1), OsIAA = OsIAA14 (LOC_Os03g58350.1). [*** (p value < 0.001), ** (p value 0.001 to 0.01) & * (p value 0.01 to 0.05)].
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f0015: Validation of the microarray data with chosen genes: cDNA was prepared from total RNA isolated from shoots of control (0 h), 6 h, 24 h R. mucilaginosa treated rice plants. BAK1 = Brassinosteroid insensitive 1-associated receptor kinase 1 precursor (LOC_Os11g31540), GDSL like lipase (LOC_Os02g57110), GSGOGAT = glutamate synthase (LOC_Os05g48200.1), OsIAA = OsIAA14 (LOC_Os03g58350.1). [*** (p value < 0.001), ** (p value 0.001 to 0.01) & * (p value 0.01 to 0.05)].

Mentions: The validation was performed using RNA isolated from tissue from a different biological pool using genes randomly chosen. Genes used were Brassinosteroid insensitive 1 associated receptor kinase 1 (LOC_Os11g31540), GDSL like lipase (LOC_Os02g57110), glutamate synthase (GS-GOGAT, LOC_Os05g48200), OsIAA14 (LOC_Os03g58350) (Fig. 3). All these genes showed similar expression patterns as observed in the microarray analysis validating the data (Fig. 3).


Early changes in shoot transcriptome of rice in response to Rhodotorula mucilaginosa JGTA-S1.

Saha C, Seal A - Genom Data (2015)

Validation of the microarray data with chosen genes: cDNA was prepared from total RNA isolated from shoots of control (0 h), 6 h, 24 h R. mucilaginosa treated rice plants. BAK1 = Brassinosteroid insensitive 1-associated receptor kinase 1 precursor (LOC_Os11g31540), GDSL like lipase (LOC_Os02g57110), GSGOGAT = glutamate synthase (LOC_Os05g48200.1), OsIAA = OsIAA14 (LOC_Os03g58350.1). [*** (p value < 0.001), ** (p value 0.001 to 0.01) & * (p value 0.01 to 0.05)].
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4664776&req=5

f0015: Validation of the microarray data with chosen genes: cDNA was prepared from total RNA isolated from shoots of control (0 h), 6 h, 24 h R. mucilaginosa treated rice plants. BAK1 = Brassinosteroid insensitive 1-associated receptor kinase 1 precursor (LOC_Os11g31540), GDSL like lipase (LOC_Os02g57110), GSGOGAT = glutamate synthase (LOC_Os05g48200.1), OsIAA = OsIAA14 (LOC_Os03g58350.1). [*** (p value < 0.001), ** (p value 0.001 to 0.01) & * (p value 0.01 to 0.05)].
Mentions: The validation was performed using RNA isolated from tissue from a different biological pool using genes randomly chosen. Genes used were Brassinosteroid insensitive 1 associated receptor kinase 1 (LOC_Os11g31540), GDSL like lipase (LOC_Os02g57110), glutamate synthase (GS-GOGAT, LOC_Os05g48200), OsIAA14 (LOC_Os03g58350) (Fig. 3). All these genes showed similar expression patterns as observed in the microarray analysis validating the data (Fig. 3).

Bottom Line: Some of the Rhodotorula species are found to exhibit plant growth promoting activities and also have been reported to protect plants against invading pathogens.Transcriptional changes were monitored in 6 h and 24 h treated rice plant shoots as compared to 0 h control.The microarray data has been submitted to the NCBI GEO repository under the accession number of GSE64321.

View Article: PubMed Central - PubMed

Affiliation: Department of Biotechnology, Dr. B.C. Guha Centre for Genetic Engineering and Biotechnology, University of Calcutta, 35, Ballygunge Circular Road, Kolkata700019, West Bengal, India.

ABSTRACT
Yeasts of Rhodotorula genus have been reported to show endophytic colonization in different plants. Some of the Rhodotorula species are found to exhibit plant growth promoting activities and also have been reported to protect plants against invading pathogens. A yeast strain closely related to Rhodotorula mucilaginosa was isolated from the endosphere of Typha angustifolia collected from a Uranium mine. A microarray analysis was performed to investigate the early changes in rice shoot transcripts in response to this yeast (R. mucilaginosa JGTA-S1). Transcriptional changes were monitored in 6 h and 24 h treated rice plant shoots as compared to 0 h control. The microarray data has been submitted to the NCBI GEO repository under the accession number of GSE64321.

No MeSH data available.


Related in: MedlinePlus