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Illumina based whole mitochondrial genome of Junonia iphita reveals minor intraspecific variation.

Vanlalruati C, De Mandal S, Guruswami G, Nachimuthu SK - Genom Data (2015)

Bottom Line: The gene order and orientation are identical to those in other butterfly species.The phylogenetic tree constructed from the whole mitogenomes using the 13 protein coding genes (PCGs) defines the genetic relatedness of the two J. iphita species collected from two different regions.All the Junonia species clustered together, and were further subdivided into clade one consisting of J. almana and J. orithya and clade two comprising of the two J. iphita which were collected from Indo and Indochinese subregions separated by river barrier.

View Article: PubMed Central - PubMed

Affiliation: Departments of Biotechnology and Zoology, Mizoram University, Aizawl, Mizoram 796004, India.

ABSTRACT
In the present study, the near complete mitochondrial genome (mitogenome) of Junonia iphita (Lepidoptera: Nymphalidae: Nymphalinae) was determined to be 14,892 bp. The gene order and orientation are identical to those in other butterfly species. The phylogenetic tree constructed from the whole mitogenomes using the 13 protein coding genes (PCGs) defines the genetic relatedness of the two J. iphita species collected from two different regions. All the Junonia species clustered together, and were further subdivided into clade one consisting of J. almana and J. orithya and clade two comprising of the two J. iphita which were collected from Indo and Indochinese subregions separated by river barrier. Comparison between the two J. iphita sequences revealed minor variations and Single Nucleotide Polymorphisms were identified at 51 sites amounting to 0.4% of the entire mitochondrial genome.

No MeSH data available.


Related in: MedlinePlus

Maximum Parsimony tree of whole mitochondrial genome using PAUP Software.Number of bootstrap replicates = 1000 Starting tree(s) obtained via stepwise random addition, number of trees held at each step during stepwise addition, branch-swapping algorithm: tree-bisection-reconnection (TBR).
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f0005: Maximum Parsimony tree of whole mitochondrial genome using PAUP Software.Number of bootstrap replicates = 1000 Starting tree(s) obtained via stepwise random addition, number of trees held at each step during stepwise addition, branch-swapping algorithm: tree-bisection-reconnection (TBR).

Mentions: The phylogenetic tree was constructed for the whole mitochondrial genome using MP method (Fig. 1). In order to study the phylogenetic relationship of the two J. iphita samples in relation to other butterfly mitogenomes, a dataset containing concatenated 13 protein coding genes was generated. The final alignment contains 13,269 sites in the matrix with 18 in-group and one out-group taxa. Of these sites, 7547 conserved, 5713 variables and 3963 parsimony informative sites were found. In MP analysis, two heliconiinae species clustered together and limentidinae form the basal clade. Within Nymphalinae subfamily, the four Junonia species clustered together with high bootstrap support, which further subdivided into two clade, one clade consist of the J. almana and J. orithya; the other clade comprising the two J. iphita individuals. The phylogenetic tree constructed for the whole mitogenomes using the 13 PCGs region defines the genetic relatedness of the two J. iphita species.


Illumina based whole mitochondrial genome of Junonia iphita reveals minor intraspecific variation.

Vanlalruati C, De Mandal S, Guruswami G, Nachimuthu SK - Genom Data (2015)

Maximum Parsimony tree of whole mitochondrial genome using PAUP Software.Number of bootstrap replicates = 1000 Starting tree(s) obtained via stepwise random addition, number of trees held at each step during stepwise addition, branch-swapping algorithm: tree-bisection-reconnection (TBR).
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4664761&req=5

f0005: Maximum Parsimony tree of whole mitochondrial genome using PAUP Software.Number of bootstrap replicates = 1000 Starting tree(s) obtained via stepwise random addition, number of trees held at each step during stepwise addition, branch-swapping algorithm: tree-bisection-reconnection (TBR).
Mentions: The phylogenetic tree was constructed for the whole mitochondrial genome using MP method (Fig. 1). In order to study the phylogenetic relationship of the two J. iphita samples in relation to other butterfly mitogenomes, a dataset containing concatenated 13 protein coding genes was generated. The final alignment contains 13,269 sites in the matrix with 18 in-group and one out-group taxa. Of these sites, 7547 conserved, 5713 variables and 3963 parsimony informative sites were found. In MP analysis, two heliconiinae species clustered together and limentidinae form the basal clade. Within Nymphalinae subfamily, the four Junonia species clustered together with high bootstrap support, which further subdivided into two clade, one clade consist of the J. almana and J. orithya; the other clade comprising the two J. iphita individuals. The phylogenetic tree constructed for the whole mitogenomes using the 13 PCGs region defines the genetic relatedness of the two J. iphita species.

Bottom Line: The gene order and orientation are identical to those in other butterfly species.The phylogenetic tree constructed from the whole mitogenomes using the 13 protein coding genes (PCGs) defines the genetic relatedness of the two J. iphita species collected from two different regions.All the Junonia species clustered together, and were further subdivided into clade one consisting of J. almana and J. orithya and clade two comprising of the two J. iphita which were collected from Indo and Indochinese subregions separated by river barrier.

View Article: PubMed Central - PubMed

Affiliation: Departments of Biotechnology and Zoology, Mizoram University, Aizawl, Mizoram 796004, India.

ABSTRACT
In the present study, the near complete mitochondrial genome (mitogenome) of Junonia iphita (Lepidoptera: Nymphalidae: Nymphalinae) was determined to be 14,892 bp. The gene order and orientation are identical to those in other butterfly species. The phylogenetic tree constructed from the whole mitogenomes using the 13 protein coding genes (PCGs) defines the genetic relatedness of the two J. iphita species collected from two different regions. All the Junonia species clustered together, and were further subdivided into clade one consisting of J. almana and J. orithya and clade two comprising of the two J. iphita which were collected from Indo and Indochinese subregions separated by river barrier. Comparison between the two J. iphita sequences revealed minor variations and Single Nucleotide Polymorphisms were identified at 51 sites amounting to 0.4% of the entire mitochondrial genome.

No MeSH data available.


Related in: MedlinePlus