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Investigation of bacterial diversity of hot springs of Odisha, India.

Sahoo RK, Subudhi E, Kumar M - Genom Data (2015)

Bottom Line: A large number of sequences remained taxonomically unresolved in both hot springs, indicating the presence of potentially novel microbes in these two unique habitats thus unraveling the importance of the current study.Metagenome sequence information is now available at NCBI, SRA database accession no.SRP057428.

View Article: PubMed Central - PubMed

Affiliation: Centre Of Biotechnology, Siksha O Anusandhan University, Bhubaneswar, Odisha, India.

ABSTRACT
16S rRNA deep sequencing analysis, targeting V3 region was performed using Illumina bar coded sequencing. Sediment samples from two hot springs (Atri and Taptapani) were collected. Atri and Taptapani metagenomes were classified into 50 and 51 bacterial phyla. Proteobacteria (45.17%) dominated the Taptapani sample metagenome followed by Bacteriodetes (23.43%) and Cyanobacteria (10.48%) while in the Atri sample, Chloroflexi (52.39%), Nitrospirae (10.93%) and Proteobacteria (9.98%) dominated. A large number of sequences remained taxonomically unresolved in both hot springs, indicating the presence of potentially novel microbes in these two unique habitats thus unraveling the importance of the current study. Metagenome sequence information is now available at NCBI, SRA database accession no. SRP057428.

No MeSH data available.


Community composition in Taptapani hot spring metagenome
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f0010: Community composition in Taptapani hot spring metagenome

Mentions: The output data comprised 186,292 and 333,276 high quality reads for Atri and Taptapani samples respectively were used for analysis. These stringent measures removed nearly 65% (Atri) and 51% (Taptapani) of the initial reads. At 80% confidence threshold in the RDP classifier, the 16S rRNA gene sequencing reads of Atri and Taptapani samples were classified into 50 and 51 bacterial phyla. In the Atri sample, most sequences were affiliated predominantly with Chloroflexi (52.39%), Nitrospirae (10.93%), Proteobacteria (9.98%), [Thermi] (6.84%), Actinobacteria (5.19%), Acidobacteria (3.84%), Armatimonadetes (3.09%) and Firmicutes (2.09%) that together accounted for ~ 95% of all sequence reads (Fig. 1). On the other hand, Proteobacteria dominated the Taptapani sample (45.17%) followed by Bacteriodetes (23.43%) and Cyanobacteria (10.48%) (Fig. 2). The other dominant phyla in Taptapani sample were Chloroflexi (7.16%), Firmicutes (2.76%) and Actinobacteria (2.44%) (Fig. 2). Out of 814 genera assigned to Taptapani hot spring, only 51% were identified at the genus level. The same trend was followed in Atri sample.


Investigation of bacterial diversity of hot springs of Odisha, India.

Sahoo RK, Subudhi E, Kumar M - Genom Data (2015)

Community composition in Taptapani hot spring metagenome
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4664759&req=5

f0010: Community composition in Taptapani hot spring metagenome
Mentions: The output data comprised 186,292 and 333,276 high quality reads for Atri and Taptapani samples respectively were used for analysis. These stringent measures removed nearly 65% (Atri) and 51% (Taptapani) of the initial reads. At 80% confidence threshold in the RDP classifier, the 16S rRNA gene sequencing reads of Atri and Taptapani samples were classified into 50 and 51 bacterial phyla. In the Atri sample, most sequences were affiliated predominantly with Chloroflexi (52.39%), Nitrospirae (10.93%), Proteobacteria (9.98%), [Thermi] (6.84%), Actinobacteria (5.19%), Acidobacteria (3.84%), Armatimonadetes (3.09%) and Firmicutes (2.09%) that together accounted for ~ 95% of all sequence reads (Fig. 1). On the other hand, Proteobacteria dominated the Taptapani sample (45.17%) followed by Bacteriodetes (23.43%) and Cyanobacteria (10.48%) (Fig. 2). The other dominant phyla in Taptapani sample were Chloroflexi (7.16%), Firmicutes (2.76%) and Actinobacteria (2.44%) (Fig. 2). Out of 814 genera assigned to Taptapani hot spring, only 51% were identified at the genus level. The same trend was followed in Atri sample.

Bottom Line: A large number of sequences remained taxonomically unresolved in both hot springs, indicating the presence of potentially novel microbes in these two unique habitats thus unraveling the importance of the current study.Metagenome sequence information is now available at NCBI, SRA database accession no.SRP057428.

View Article: PubMed Central - PubMed

Affiliation: Centre Of Biotechnology, Siksha O Anusandhan University, Bhubaneswar, Odisha, India.

ABSTRACT
16S rRNA deep sequencing analysis, targeting V3 region was performed using Illumina bar coded sequencing. Sediment samples from two hot springs (Atri and Taptapani) were collected. Atri and Taptapani metagenomes were classified into 50 and 51 bacterial phyla. Proteobacteria (45.17%) dominated the Taptapani sample metagenome followed by Bacteriodetes (23.43%) and Cyanobacteria (10.48%) while in the Atri sample, Chloroflexi (52.39%), Nitrospirae (10.93%) and Proteobacteria (9.98%) dominated. A large number of sequences remained taxonomically unresolved in both hot springs, indicating the presence of potentially novel microbes in these two unique habitats thus unraveling the importance of the current study. Metagenome sequence information is now available at NCBI, SRA database accession no. SRP057428.

No MeSH data available.