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Expression profiling of human melanocytes in response to UV-B irradiation.

López S, Smith-Zubiaga I, Alonso S - Genom Data (2015)

Bottom Line: A comprehensive gene expression analysis of human melanocytes was performed assessing the transcriptional profile of dark melanocytes (DM) and light melanocytes (LM) at basal conditions and after UV-B irradiation at different time points (6, 12 and 24 h), and in culture with different keratinocyte-conditioned media (KCM + and KCM -).The data, previously published in [1], have been deposited in NCBI's Gene Expression Omnibus (GEO accession number: GSE70280).

View Article: PubMed Central - PubMed

Affiliation: Department of Genetics, Evolution and Environment, University College London, London, United Kingdom.

ABSTRACT
A comprehensive gene expression analysis of human melanocytes was performed assessing the transcriptional profile of dark melanocytes (DM) and light melanocytes (LM) at basal conditions and after UV-B irradiation at different time points (6, 12 and 24 h), and in culture with different keratinocyte-conditioned media (KCM + and KCM -). The data, previously published in [1], have been deposited in NCBI's Gene Expression Omnibus (GEO accession number: GSE70280).

No MeSH data available.


Related in: MedlinePlus

Experimental design.
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f0005: Experimental design.

Mentions: Human epidermal keratinocytes (HEK) were purchased from Cascade Biologics. HEKs were maintained in EpiLife Medium supplemented with human keratinocyte growth supplement (HKGS) consisting of 0.2 ng ml− 1 human recombinant epidermal growth factor (EGF), 0.18 μg ml− 1 hydrocortisone, 5 μg ml− 1 insulin, 5 μg ml− 1 transferrin, and 0.2% (v/v) bovine pituitary extract. Cell cultures were maintained in an incubator under an atmosphere of 5% CO2 at 37 °C. Media were refreshed every two days and cells from the third to fifth passage were used. Subconfluent cultures were then irradiated at 75 mJ/cm2 UV-B in an ICH2 photoreactor (LuzChem, Canada) at 37 °C. Prior to irradiation, the culture medium was replaced with phosphate buffer saline (PBS) and immediately after irradiation PBS was replaced again with culture medium. 24 h after irradiation supernatant (KCM +), was collected. The non-irradiated keratinocytes' supernatant was also harvested as a control (KCM‐) (Fig. 1).


Expression profiling of human melanocytes in response to UV-B irradiation.

López S, Smith-Zubiaga I, Alonso S - Genom Data (2015)

Experimental design.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4664746&req=5

f0005: Experimental design.
Mentions: Human epidermal keratinocytes (HEK) were purchased from Cascade Biologics. HEKs were maintained in EpiLife Medium supplemented with human keratinocyte growth supplement (HKGS) consisting of 0.2 ng ml− 1 human recombinant epidermal growth factor (EGF), 0.18 μg ml− 1 hydrocortisone, 5 μg ml− 1 insulin, 5 μg ml− 1 transferrin, and 0.2% (v/v) bovine pituitary extract. Cell cultures were maintained in an incubator under an atmosphere of 5% CO2 at 37 °C. Media were refreshed every two days and cells from the third to fifth passage were used. Subconfluent cultures were then irradiated at 75 mJ/cm2 UV-B in an ICH2 photoreactor (LuzChem, Canada) at 37 °C. Prior to irradiation, the culture medium was replaced with phosphate buffer saline (PBS) and immediately after irradiation PBS was replaced again with culture medium. 24 h after irradiation supernatant (KCM +), was collected. The non-irradiated keratinocytes' supernatant was also harvested as a control (KCM‐) (Fig. 1).

Bottom Line: A comprehensive gene expression analysis of human melanocytes was performed assessing the transcriptional profile of dark melanocytes (DM) and light melanocytes (LM) at basal conditions and after UV-B irradiation at different time points (6, 12 and 24 h), and in culture with different keratinocyte-conditioned media (KCM + and KCM -).The data, previously published in [1], have been deposited in NCBI's Gene Expression Omnibus (GEO accession number: GSE70280).

View Article: PubMed Central - PubMed

Affiliation: Department of Genetics, Evolution and Environment, University College London, London, United Kingdom.

ABSTRACT
A comprehensive gene expression analysis of human melanocytes was performed assessing the transcriptional profile of dark melanocytes (DM) and light melanocytes (LM) at basal conditions and after UV-B irradiation at different time points (6, 12 and 24 h), and in culture with different keratinocyte-conditioned media (KCM + and KCM -). The data, previously published in [1], have been deposited in NCBI's Gene Expression Omnibus (GEO accession number: GSE70280).

No MeSH data available.


Related in: MedlinePlus