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Microarray analyses during early stage of the tomato/Alternaria solani interaction.

Upadhyay P, Rai A, Kumar R, Singh M, Sinha B - Genom Data (2015)

Bottom Line: Tomato early blight is an important threat and it has capacity to reduce the production in all major tomato producing areas.Molecular mechanism underlying the resistance against this is not well known.Their differential expressions have enhanced the biochemical and other related products, which have, direct or indirect role in stopping the penetration of mycelia in the host plant.

View Article: PubMed Central - PubMed

Affiliation: Department of Botany, University of Delhi, Delhi 110 007, India ; Indian Institute of Vegetable Research, Varanasi 221 305, India ; Department of Genetics and Plant Breeding, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi 221 005, India.

ABSTRACT
Tomato early blight is an important threat and it has capacity to reduce the production in all major tomato producing areas. Molecular mechanism underlying the resistance against this is not well known. Therefore we studied this system to search the possible mechanism of resistance, which includes pathogenesis related protein, and pathways and transcription factors, which are responsible for resistance against this pathogen using affymetrix gene chip for tomato. Their differential expressions have enhanced the biochemical and other related products, which have, direct or indirect role in stopping the penetration of mycelia in the host plant.

No MeSH data available.


Related in: MedlinePlus

Schematic presentation of expression profiling experiment.
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f0005: Schematic presentation of expression profiling experiment.

Mentions: Double stranded cDNA synthesis, in vitro transcription to synthesize biotin labeled aRNA, purification and fragmentation of aRNA, and hybridization of arrays were performed according to Affymetrix technical manual. The Affymetrix GeneChip Tomato Genome Array contains 10, 038 probe sets, representing about 4600 unigenes. The hybridized chips were washed, stained and scanned using the GeneChip scanner to generate the CEL files. Fig. 1 shows the schematic presentation of the experiment.


Microarray analyses during early stage of the tomato/Alternaria solani interaction.

Upadhyay P, Rai A, Kumar R, Singh M, Sinha B - Genom Data (2015)

Schematic presentation of expression profiling experiment.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4664743&req=5

f0005: Schematic presentation of expression profiling experiment.
Mentions: Double stranded cDNA synthesis, in vitro transcription to synthesize biotin labeled aRNA, purification and fragmentation of aRNA, and hybridization of arrays were performed according to Affymetrix technical manual. The Affymetrix GeneChip Tomato Genome Array contains 10, 038 probe sets, representing about 4600 unigenes. The hybridized chips were washed, stained and scanned using the GeneChip scanner to generate the CEL files. Fig. 1 shows the schematic presentation of the experiment.

Bottom Line: Tomato early blight is an important threat and it has capacity to reduce the production in all major tomato producing areas.Molecular mechanism underlying the resistance against this is not well known.Their differential expressions have enhanced the biochemical and other related products, which have, direct or indirect role in stopping the penetration of mycelia in the host plant.

View Article: PubMed Central - PubMed

Affiliation: Department of Botany, University of Delhi, Delhi 110 007, India ; Indian Institute of Vegetable Research, Varanasi 221 305, India ; Department of Genetics and Plant Breeding, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi 221 005, India.

ABSTRACT
Tomato early blight is an important threat and it has capacity to reduce the production in all major tomato producing areas. Molecular mechanism underlying the resistance against this is not well known. Therefore we studied this system to search the possible mechanism of resistance, which includes pathogenesis related protein, and pathways and transcription factors, which are responsible for resistance against this pathogen using affymetrix gene chip for tomato. Their differential expressions have enhanced the biochemical and other related products, which have, direct or indirect role in stopping the penetration of mycelia in the host plant.

No MeSH data available.


Related in: MedlinePlus