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An approach to identify the novel miRNA encoded from H. Annuus EST sequences.

Gupta H, Tiwari T, Patel M, Mehta A, Ghosh A - Genom Data (2015)

Bottom Line: A total of three miRNAs were detected from which one potential novel miRNA was identified following a range of strict filtering criteria.To study the conserved nature of the miRNAs, predicted phylogenetic analysis was carried out.These findings will significantly provide the broader picture for understanding the functions in H. annuus.

View Article: PubMed Central - PubMed

Affiliation: Xcelris Labs Ltd., Ahmedabad, Gujarat, India.

ABSTRACT
MicroRNAs are a newly discovered class of non-protein small RNAs with 22-24 nucleotides. They play multiple roles in biological processes including development, cell proliferation, apoptosis, stress responses and many other cell functions. In this research, several approaches were combined to make a computational prediction of potential miRNAs and their targets in Helianthus annuus (H. annuus). The already available information of the plant miRNAs present in miRBase v21 was used against expressed sequence tags (ESTs). A total of three miRNAs were detected from which one potential novel miRNA was identified following a range of strict filtering criteria. The target prediction was carried out for these three miRNAs having various targets. These targets were functionally annotated and GO terms were assigned. To study the conserved nature of the miRNAs, predicted phylogenetic analysis was carried out. These findings will significantly provide the broader picture for understanding the functions in H. annuus.

No MeSH data available.


Related in: MedlinePlus

GO distributions of miRNA targets among the EST and Unigene data sets.
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f0015: GO distributions of miRNA targets among the EST and Unigene data sets.

Mentions: The targets were functionally annotated, followed by GO annotation. These miRNA targets belonged to a number of gene families that are involved in different biological functions. The miRNA family miR396 showed the highest 32 numbers of independent targets followed by other two families; simultaneously 8 targets were annotated for biological process and cellular component and 10 for molecular function from three miRNA families as concluded in Fig. 3.


An approach to identify the novel miRNA encoded from H. Annuus EST sequences.

Gupta H, Tiwari T, Patel M, Mehta A, Ghosh A - Genom Data (2015)

GO distributions of miRNA targets among the EST and Unigene data sets.
© Copyright Policy - CC BY
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4664742&req=5

f0015: GO distributions of miRNA targets among the EST and Unigene data sets.
Mentions: The targets were functionally annotated, followed by GO annotation. These miRNA targets belonged to a number of gene families that are involved in different biological functions. The miRNA family miR396 showed the highest 32 numbers of independent targets followed by other two families; simultaneously 8 targets were annotated for biological process and cellular component and 10 for molecular function from three miRNA families as concluded in Fig. 3.

Bottom Line: A total of three miRNAs were detected from which one potential novel miRNA was identified following a range of strict filtering criteria.To study the conserved nature of the miRNAs, predicted phylogenetic analysis was carried out.These findings will significantly provide the broader picture for understanding the functions in H. annuus.

View Article: PubMed Central - PubMed

Affiliation: Xcelris Labs Ltd., Ahmedabad, Gujarat, India.

ABSTRACT
MicroRNAs are a newly discovered class of non-protein small RNAs with 22-24 nucleotides. They play multiple roles in biological processes including development, cell proliferation, apoptosis, stress responses and many other cell functions. In this research, several approaches were combined to make a computational prediction of potential miRNAs and their targets in Helianthus annuus (H. annuus). The already available information of the plant miRNAs present in miRBase v21 was used against expressed sequence tags (ESTs). A total of three miRNAs were detected from which one potential novel miRNA was identified following a range of strict filtering criteria. The target prediction was carried out for these three miRNAs having various targets. These targets were functionally annotated and GO terms were assigned. To study the conserved nature of the miRNAs, predicted phylogenetic analysis was carried out. These findings will significantly provide the broader picture for understanding the functions in H. annuus.

No MeSH data available.


Related in: MedlinePlus