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Gene expression profiling of rice seedlings in response to glutamine treatment.

Kan CC, Chung TY, Hsieh MH - Genom Data (2015)

Bottom Line: In addition to metabolic and nutritional effects, glutamine may function as a signaling molecule to regulate gene expression in plants.The results revealed that glutamine induced the expression of at least 35 genes involved in metabolism, transport, signal transduction, and stress responses within 30 min (Kan et al., 2015 [4]).Here, we provide the details of the experimental procedure associated with our microarray data deposited in NCBI's Gene Expression Omnibus (GEO ID: GSE56770).

View Article: PubMed Central - PubMed

Affiliation: Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan.

ABSTRACT
Glutamine, the most abundant free amino acid in humans (Curi et al., 2007 [1]), has many functions. In addition to protein, amino acid, and nucleic acid biosynthesis, glutamine also regulates the expression of genes related to metabolism, cell defense, and signal transduction in humans (Curi et al., 2007 [1]; Brasse-Lagnel et al., 2009 [2]). Glutamine is also one of the major forms of nitrogen in rice (Fukumorita and Chino, 1982 [3]). In addition to metabolic and nutritional effects, glutamine may function as a signaling molecule to regulate gene expression in plants. To this end, we used microarray analysis to identify genes that are rapidly induced by 2.5 mM glutamine in rice roots. The results revealed that glutamine induced the expression of at least 35 genes involved in metabolism, transport, signal transduction, and stress responses within 30 min (Kan et al., 2015 [4]). Here, we provide the details of the experimental procedure associated with our microarray data deposited in NCBI's Gene Expression Omnibus (GEO ID: GSE56770).

No MeSH data available.


Schematic diagram of the steps involved in this study. Roots and shoots were harvested separately for total RNA isolation. Two independent sets of biological samples were used for the microarray analysis using Affymetrix Rice Genome GeneChip.
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f0005: Schematic diagram of the steps involved in this study. Roots and shoots were harvested separately for total RNA isolation. Two independent sets of biological samples were used for the microarray analysis using Affymetrix Rice Genome GeneChip.

Mentions: To identify genes that are rapidly regulated by glutamine, 17-day-old rice seedlings grown in hydroponic solution [5] without nitrogen (− N) were treated with 2.5 mM glutamine for 30 min (+ Gln). Total RNAs isolated from − N and + Gln rice roots were used to perform gene expression profiling with the Affymetrix GeneChip Rice Genome Array (Fig. 1).


Gene expression profiling of rice seedlings in response to glutamine treatment.

Kan CC, Chung TY, Hsieh MH - Genom Data (2015)

Schematic diagram of the steps involved in this study. Roots and shoots were harvested separately for total RNA isolation. Two independent sets of biological samples were used for the microarray analysis using Affymetrix Rice Genome GeneChip.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4664718&req=5

f0005: Schematic diagram of the steps involved in this study. Roots and shoots were harvested separately for total RNA isolation. Two independent sets of biological samples were used for the microarray analysis using Affymetrix Rice Genome GeneChip.
Mentions: To identify genes that are rapidly regulated by glutamine, 17-day-old rice seedlings grown in hydroponic solution [5] without nitrogen (− N) were treated with 2.5 mM glutamine for 30 min (+ Gln). Total RNAs isolated from − N and + Gln rice roots were used to perform gene expression profiling with the Affymetrix GeneChip Rice Genome Array (Fig. 1).

Bottom Line: In addition to metabolic and nutritional effects, glutamine may function as a signaling molecule to regulate gene expression in plants.The results revealed that glutamine induced the expression of at least 35 genes involved in metabolism, transport, signal transduction, and stress responses within 30 min (Kan et al., 2015 [4]).Here, we provide the details of the experimental procedure associated with our microarray data deposited in NCBI's Gene Expression Omnibus (GEO ID: GSE56770).

View Article: PubMed Central - PubMed

Affiliation: Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan.

ABSTRACT
Glutamine, the most abundant free amino acid in humans (Curi et al., 2007 [1]), has many functions. In addition to protein, amino acid, and nucleic acid biosynthesis, glutamine also regulates the expression of genes related to metabolism, cell defense, and signal transduction in humans (Curi et al., 2007 [1]; Brasse-Lagnel et al., 2009 [2]). Glutamine is also one of the major forms of nitrogen in rice (Fukumorita and Chino, 1982 [3]). In addition to metabolic and nutritional effects, glutamine may function as a signaling molecule to regulate gene expression in plants. To this end, we used microarray analysis to identify genes that are rapidly induced by 2.5 mM glutamine in rice roots. The results revealed that glutamine induced the expression of at least 35 genes involved in metabolism, transport, signal transduction, and stress responses within 30 min (Kan et al., 2015 [4]). Here, we provide the details of the experimental procedure associated with our microarray data deposited in NCBI's Gene Expression Omnibus (GEO ID: GSE56770).

No MeSH data available.