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MicroRNA-431 inhibits migration and invasion of hepatocellular carcinoma cells by targeting the ZEB1-mediated epithelial-mensenchymal transition.

Sun K, Zeng T, Huang D, Liu Z, Huang S, Liu J, Qu Z - FEBS Open Bio (2015)

Bottom Line: Herein, we found that miR-431 expression was reduced in HCC tissues compared to noncancerous tissues.We found that up-regulation of miR-431 expression decreased zinc finger E-box binding homeobox 1 (ZEB1) expression and inhibited the epithelial-mesenchymal transition (EMT) with increased E-cadherin expression and decreased vimentin expression in HCCLM3 cells.In conclusion, miR-431 inhibits migration and invasion of HCC cells by suppressing ZEB1-mediated EMT.

View Article: PubMed Central - PubMed

Affiliation: Department of General Surgery, Taihe Hospital, Hubei University of Medicine, Shiyan 442000, China.

ABSTRACT
MicroRNA-431 (miR-431) has been recognized as an oncogenic miRNA, being implicated in the initiation and development of human cancers. Recently, deregulation of miR-431 has been reported in several tumors. However, the clinical significance of miR-431 and its underlying role in human hepatocellular carcinoma (HCC) are poorly explored. Herein, we found that miR-431 expression was reduced in HCC tissues compared to noncancerous tissues. Otherwise, down-regulation of miR-431 was observed in aggressive tumor tissues. The levels of miR-431 expression in HCC cell lines were significantly lower than that in a nontransformed hepatic cell line. Clinical association analyses disclosed that a low level of miR-431 was prominently associated with poor prognostic features of HCC including venous infiltration, high Edmondson-Steiner grading and advanced tumor-node-metastasis (TNM) tumor stage. Our in vitro studies showed that up-regulation of miR-431 expression reduced cell invasion and migration in HCCLM3 cells. In contrast, down-regulation of miR-431 expression promoted SMMC-7721 cell invasion and migration. We found that up-regulation of miR-431 expression decreased zinc finger E-box binding homeobox 1 (ZEB1) expression and inhibited the epithelial-mesenchymal transition (EMT) with increased E-cadherin expression and decreased vimentin expression in HCCLM3 cells. Otherwise, down-regulation of miR-431 expression increased ZEB1 expression and promoted EMT in SMMC-7721 cells. Significantly, ZEB1 was identified as a target of miR-431 in HCC. ZEB1 knockdown abrogated the effect of miR-431 silencing on EMT and cell mobility in SMMC-7721 cells. In conclusion, miR-431 inhibits migration and invasion of HCC cells by suppressing ZEB1-mediated EMT.

No MeSH data available.


Related in: MedlinePlus

MiR-431 does not affect HCC cell viability. (A) HCCLM3 cells that were transfected with miR-431 or miR-control were subjected to MTT assays. MiR-431 overexpression did not significantly affect cell viability. n = 3. (B) SMMC-7721 cells that were transduced with miR-431 inhibitor (anti-miR-431) or negative control (NC). As assessed by MTT assays, miR-431 silencing was not found to prominently change the viability of SMMC-7721 cells. n = 3.
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f0030: MiR-431 does not affect HCC cell viability. (A) HCCLM3 cells that were transfected with miR-431 or miR-control were subjected to MTT assays. MiR-431 overexpression did not significantly affect cell viability. n = 3. (B) SMMC-7721 cells that were transduced with miR-431 inhibitor (anti-miR-431) or negative control (NC). As assessed by MTT assays, miR-431 silencing was not found to prominently change the viability of SMMC-7721 cells. n = 3.


MicroRNA-431 inhibits migration and invasion of hepatocellular carcinoma cells by targeting the ZEB1-mediated epithelial-mensenchymal transition.

Sun K, Zeng T, Huang D, Liu Z, Huang S, Liu J, Qu Z - FEBS Open Bio (2015)

MiR-431 does not affect HCC cell viability. (A) HCCLM3 cells that were transfected with miR-431 or miR-control were subjected to MTT assays. MiR-431 overexpression did not significantly affect cell viability. n = 3. (B) SMMC-7721 cells that were transduced with miR-431 inhibitor (anti-miR-431) or negative control (NC). As assessed by MTT assays, miR-431 silencing was not found to prominently change the viability of SMMC-7721 cells. n = 3.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4664716&req=5

f0030: MiR-431 does not affect HCC cell viability. (A) HCCLM3 cells that were transfected with miR-431 or miR-control were subjected to MTT assays. MiR-431 overexpression did not significantly affect cell viability. n = 3. (B) SMMC-7721 cells that were transduced with miR-431 inhibitor (anti-miR-431) or negative control (NC). As assessed by MTT assays, miR-431 silencing was not found to prominently change the viability of SMMC-7721 cells. n = 3.
Bottom Line: Herein, we found that miR-431 expression was reduced in HCC tissues compared to noncancerous tissues.We found that up-regulation of miR-431 expression decreased zinc finger E-box binding homeobox 1 (ZEB1) expression and inhibited the epithelial-mesenchymal transition (EMT) with increased E-cadherin expression and decreased vimentin expression in HCCLM3 cells.In conclusion, miR-431 inhibits migration and invasion of HCC cells by suppressing ZEB1-mediated EMT.

View Article: PubMed Central - PubMed

Affiliation: Department of General Surgery, Taihe Hospital, Hubei University of Medicine, Shiyan 442000, China.

ABSTRACT
MicroRNA-431 (miR-431) has been recognized as an oncogenic miRNA, being implicated in the initiation and development of human cancers. Recently, deregulation of miR-431 has been reported in several tumors. However, the clinical significance of miR-431 and its underlying role in human hepatocellular carcinoma (HCC) are poorly explored. Herein, we found that miR-431 expression was reduced in HCC tissues compared to noncancerous tissues. Otherwise, down-regulation of miR-431 was observed in aggressive tumor tissues. The levels of miR-431 expression in HCC cell lines were significantly lower than that in a nontransformed hepatic cell line. Clinical association analyses disclosed that a low level of miR-431 was prominently associated with poor prognostic features of HCC including venous infiltration, high Edmondson-Steiner grading and advanced tumor-node-metastasis (TNM) tumor stage. Our in vitro studies showed that up-regulation of miR-431 expression reduced cell invasion and migration in HCCLM3 cells. In contrast, down-regulation of miR-431 expression promoted SMMC-7721 cell invasion and migration. We found that up-regulation of miR-431 expression decreased zinc finger E-box binding homeobox 1 (ZEB1) expression and inhibited the epithelial-mesenchymal transition (EMT) with increased E-cadherin expression and decreased vimentin expression in HCCLM3 cells. Otherwise, down-regulation of miR-431 expression increased ZEB1 expression and promoted EMT in SMMC-7721 cells. Significantly, ZEB1 was identified as a target of miR-431 in HCC. ZEB1 knockdown abrogated the effect of miR-431 silencing on EMT and cell mobility in SMMC-7721 cells. In conclusion, miR-431 inhibits migration and invasion of HCC cells by suppressing ZEB1-mediated EMT.

No MeSH data available.


Related in: MedlinePlus