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Transcriptomic profiling of human embryonic stem cells upon cell cycle manipulation during pluripotent state dissolution.

Gonzales KA, Liang H - Genom Data (2015)

Bottom Line: To understand the mechanisms behind the effect on PSD by these pathways in hESCs, we performed comprehensive gene expression analysis by time-course microarray experiments.From these datasets, we observed expression changes in genes involved in the TGFβ signaling pathway, which has a well-established role in hESC maintenance [3], [4], [5].The microarray data have been deposited in NCBI's Gene Expression Omnibus (GEO) and can be accessed through GEO Series accession numbers GSE62062 and GSE63215.

View Article: PubMed Central - PubMed

Affiliation: Stem Cell and Regenerative Biology, Genome Institute of Singapore, 60 Biopolis Street, Singapore 138672, Singapore.

ABSTRACT
While distinct cell cycle structures have been known to correlate with pluripotent or differentiated cell states [1], there is no evidence on how the cell cycle machinery directly contributes to human embryonic stem cell (hESC) pluripotency. We established a determinant role of cell cycle machineries on the pluripotent state by demonstrating that the specific perturbation of the S and G2 phases can prevent pluripotent state dissolution (PSD) [2]. Active mechanisms in these phases, such as the DNA damage checkpoint and Cyclin B1, promote the pluripotent state [2]. To understand the mechanisms behind the effect on PSD by these pathways in hESCs, we performed comprehensive gene expression analysis by time-course microarray experiments. From these datasets, we observed expression changes in genes involved in the TGFβ signaling pathway, which has a well-established role in hESC maintenance [3], [4], [5]. The microarray data have been deposited in NCBI's Gene Expression Omnibus (GEO) and can be accessed through GEO Series accession numbers GSE62062 and GSE63215.

No MeSH data available.


Related in: MedlinePlus

Outline of time-course experiment prior to microarray analysis.
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f0005: Outline of time-course experiment prior to microarray analysis.

Mentions: Prior to all treatments, hESCs were instead passaged as single cells using TrypLE™ Express (Life Technologies). 2 h after passage, treatments were applied. 24 h after treatment, medium was replaced to mTeSR1 without select growth factors (STEMCELL Technologies) to induce PSD (Fig. 1).


Transcriptomic profiling of human embryonic stem cells upon cell cycle manipulation during pluripotent state dissolution.

Gonzales KA, Liang H - Genom Data (2015)

Outline of time-course experiment prior to microarray analysis.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4664715&req=5

f0005: Outline of time-course experiment prior to microarray analysis.
Mentions: Prior to all treatments, hESCs were instead passaged as single cells using TrypLE™ Express (Life Technologies). 2 h after passage, treatments were applied. 24 h after treatment, medium was replaced to mTeSR1 without select growth factors (STEMCELL Technologies) to induce PSD (Fig. 1).

Bottom Line: To understand the mechanisms behind the effect on PSD by these pathways in hESCs, we performed comprehensive gene expression analysis by time-course microarray experiments.From these datasets, we observed expression changes in genes involved in the TGFβ signaling pathway, which has a well-established role in hESC maintenance [3], [4], [5].The microarray data have been deposited in NCBI's Gene Expression Omnibus (GEO) and can be accessed through GEO Series accession numbers GSE62062 and GSE63215.

View Article: PubMed Central - PubMed

Affiliation: Stem Cell and Regenerative Biology, Genome Institute of Singapore, 60 Biopolis Street, Singapore 138672, Singapore.

ABSTRACT
While distinct cell cycle structures have been known to correlate with pluripotent or differentiated cell states [1], there is no evidence on how the cell cycle machinery directly contributes to human embryonic stem cell (hESC) pluripotency. We established a determinant role of cell cycle machineries on the pluripotent state by demonstrating that the specific perturbation of the S and G2 phases can prevent pluripotent state dissolution (PSD) [2]. Active mechanisms in these phases, such as the DNA damage checkpoint and Cyclin B1, promote the pluripotent state [2]. To understand the mechanisms behind the effect on PSD by these pathways in hESCs, we performed comprehensive gene expression analysis by time-course microarray experiments. From these datasets, we observed expression changes in genes involved in the TGFβ signaling pathway, which has a well-established role in hESC maintenance [3], [4], [5]. The microarray data have been deposited in NCBI's Gene Expression Omnibus (GEO) and can be accessed through GEO Series accession numbers GSE62062 and GSE63215.

No MeSH data available.


Related in: MedlinePlus