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Prevalence and Characterization of Shiga Toxin-Producing and Enteropathogenic Escherichia coli in Shellfish-Harvesting Areas and Their Watersheds.

Balière C, Rincé A, Blanco J, Dahbi G, Harel J, Vogeleer P, Giard JC, Mariani-Kurkdjian P, Gourmelon M - Front Microbiol (2015)

Bottom Line: more strains formed a strong biofilm at 18 than at 30°C.Finally, more than 85% of analyzed strains were found to be sensitive to the 16 tested antibiotics.These data suggest the low risk of human infection by STEC if shellfish from these shellfish-harvesting areas were consumed.

View Article: PubMed Central - PubMed

Affiliation: Laboratoire Santé Environnement et Microbiologie, Unité Santé, Génétique et Microbiologie des Mollusques, Département Ressources Biologiques et Environnement, Ifremer Plouzané, France.

ABSTRACT
more strains formed a strong biofilm at 18 than at 30°C. Finally, more than 85% of analyzed strains were found to be sensitive to the 16 tested antibiotics. These data suggest the low risk of human infection by STEC if shellfish from these shellfish-harvesting areas were consumed.

No MeSH data available.


Related in: MedlinePlus

Biofilm formation by EPEC and STEC strains at low and high temperatures. Biofilms were formed on polystyrene in M9 medium supplemented with glucose (0.4% wt/vol) at 18°C (gray bars) or 30°C (dark bars) for 24 h and were stained with crystal violet, and the absorbance at 590 nm was measured. The results are the average of at least three biological replicates and the error bar represent the standard error. The results for the static biofilms formed at 18 and 30°C were compared using two-way analysis of variance (ANOVA) followed by a Bonferroni post hoc comparison. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001. astx2+, bstx1+, cstx1+-stx2+, dstx1+-stx2+-ehxA+-saa+, estx1+-eae+, feae+, geae+-ehxA+.
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Figure 2: Biofilm formation by EPEC and STEC strains at low and high temperatures. Biofilms were formed on polystyrene in M9 medium supplemented with glucose (0.4% wt/vol) at 18°C (gray bars) or 30°C (dark bars) for 24 h and were stained with crystal violet, and the absorbance at 590 nm was measured. The results are the average of at least three biological replicates and the error bar represent the standard error. The results for the static biofilms formed at 18 and 30°C were compared using two-way analysis of variance (ANOVA) followed by a Bonferroni post hoc comparison. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001. astx2+, bstx1+, cstx1+-stx2+, dstx1+-stx2+-ehxA+-saa+, estx1+-eae+, feae+, geae+-ehxA+.

Mentions: Biofilm formation by a subset of 13 EPEC and nine STEC strains was evaluated at 18 and 30°C on plastic surface. At both temperatures, strains varied in their ability to form biofilm (OD590 = 0.03 for the lowest, OD590 = 1.9 for the highest). In general, a large number of strains were strongly to moderately adherent and more strains formed a biofilm at 18 than at 30°C (Figure 2). Indeed, 11 of the 22 strains formed strong biofilms [serotypes O2:H32, O149:H1, ONT:H11, O91:H21, O185:H28, O26:H11 (n = 2), O145:H28 (n = 1), O103:H2 (n = 3)], six formed medium biofilms [serotypes O26:H11 (n = 3), O145:H28 (n = 2) and O125:H6] and five formed weak biofilms or no biofilm at all [serotypes O100:HNM, O154:H31, O15:H16, and O26:H11 (n = 2)]. At 30°C, 11 strains formed strong biofilms [serotypes O2:H32, O149:H1, ONT:H11, O91:H21, O185:H28, O145:H28 (n = 3), O103:H2 (n = 2) and O125:H6] but fewer (n = 2) were able to form a medium biofilm (O26:H11 and O145:H28) and nine formed a weak biofilm [serotypes O100:HNM, O15:H16, O154:H31, O26:H11 (n = 6); Figure 2]. Interestingly, all O26:H11 strains formed significantly (Mann–Whitney test) more biofilm (p < 0.05) at 18°C than at 30°C.


Prevalence and Characterization of Shiga Toxin-Producing and Enteropathogenic Escherichia coli in Shellfish-Harvesting Areas and Their Watersheds.

Balière C, Rincé A, Blanco J, Dahbi G, Harel J, Vogeleer P, Giard JC, Mariani-Kurkdjian P, Gourmelon M - Front Microbiol (2015)

Biofilm formation by EPEC and STEC strains at low and high temperatures. Biofilms were formed on polystyrene in M9 medium supplemented with glucose (0.4% wt/vol) at 18°C (gray bars) or 30°C (dark bars) for 24 h and were stained with crystal violet, and the absorbance at 590 nm was measured. The results are the average of at least three biological replicates and the error bar represent the standard error. The results for the static biofilms formed at 18 and 30°C were compared using two-way analysis of variance (ANOVA) followed by a Bonferroni post hoc comparison. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001. astx2+, bstx1+, cstx1+-stx2+, dstx1+-stx2+-ehxA+-saa+, estx1+-eae+, feae+, geae+-ehxA+.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
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Figure 2: Biofilm formation by EPEC and STEC strains at low and high temperatures. Biofilms were formed on polystyrene in M9 medium supplemented with glucose (0.4% wt/vol) at 18°C (gray bars) or 30°C (dark bars) for 24 h and were stained with crystal violet, and the absorbance at 590 nm was measured. The results are the average of at least three biological replicates and the error bar represent the standard error. The results for the static biofilms formed at 18 and 30°C were compared using two-way analysis of variance (ANOVA) followed by a Bonferroni post hoc comparison. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001. astx2+, bstx1+, cstx1+-stx2+, dstx1+-stx2+-ehxA+-saa+, estx1+-eae+, feae+, geae+-ehxA+.
Mentions: Biofilm formation by a subset of 13 EPEC and nine STEC strains was evaluated at 18 and 30°C on plastic surface. At both temperatures, strains varied in their ability to form biofilm (OD590 = 0.03 for the lowest, OD590 = 1.9 for the highest). In general, a large number of strains were strongly to moderately adherent and more strains formed a biofilm at 18 than at 30°C (Figure 2). Indeed, 11 of the 22 strains formed strong biofilms [serotypes O2:H32, O149:H1, ONT:H11, O91:H21, O185:H28, O26:H11 (n = 2), O145:H28 (n = 1), O103:H2 (n = 3)], six formed medium biofilms [serotypes O26:H11 (n = 3), O145:H28 (n = 2) and O125:H6] and five formed weak biofilms or no biofilm at all [serotypes O100:HNM, O154:H31, O15:H16, and O26:H11 (n = 2)]. At 30°C, 11 strains formed strong biofilms [serotypes O2:H32, O149:H1, ONT:H11, O91:H21, O185:H28, O145:H28 (n = 3), O103:H2 (n = 2) and O125:H6] but fewer (n = 2) were able to form a medium biofilm (O26:H11 and O145:H28) and nine formed a weak biofilm [serotypes O100:HNM, O15:H16, O154:H31, O26:H11 (n = 6); Figure 2]. Interestingly, all O26:H11 strains formed significantly (Mann–Whitney test) more biofilm (p < 0.05) at 18°C than at 30°C.

Bottom Line: more strains formed a strong biofilm at 18 than at 30°C.Finally, more than 85% of analyzed strains were found to be sensitive to the 16 tested antibiotics.These data suggest the low risk of human infection by STEC if shellfish from these shellfish-harvesting areas were consumed.

View Article: PubMed Central - PubMed

Affiliation: Laboratoire Santé Environnement et Microbiologie, Unité Santé, Génétique et Microbiologie des Mollusques, Département Ressources Biologiques et Environnement, Ifremer Plouzané, France.

ABSTRACT
more strains formed a strong biofilm at 18 than at 30°C. Finally, more than 85% of analyzed strains were found to be sensitive to the 16 tested antibiotics. These data suggest the low risk of human infection by STEC if shellfish from these shellfish-harvesting areas were consumed.

No MeSH data available.


Related in: MedlinePlus