Limits...
Altered Hippocampal Neurogenesis and Amygdalar Neuronal Activity in Adult Mice with Repeated Experience of Aggression.

Smagin DA, Park JH, Michurina TV, Peunova N, Glass Z, Sayed K, Bondar NP, Kovalenko IN, Kudryavtseva NN, Enikolopov G - Front Neurosci (2015)

Bottom Line: Positive fighting experience results in increased levels of progenitor cell proliferation and production of young neurons in the hippocampus.Furthermore, repeated winning experience decreases the number of activated (c-fos-positive) cells in the basolateral amygdala and increases the number of activated cells in the hippocampus; a subsequent no-fight period restores the number of c-fos-positive cells.Our results indicate that extended positive fighting experience in a social conflict heightens aggression, increases proliferation of neuronal progenitors and production of young neurons in the hippocampus, and decreases neuronal activity in the amygdala; these changes can be modified by depriving the winners of the opportunity for further fights.

View Article: PubMed Central - PubMed

Affiliation: Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences Novosibirsk, Russia ; Department of Nano-, Bio-, Information Technology and Cognitive Science, Moscow Institute of Physics and Technology Moscow, Russia ; Cold Spring Harbor Laboratory, Cold Spring Harbor NY, USA.

ABSTRACT
Repeated experience of winning in a social conflict setting elevates levels of aggression and may lead to violent behavioral patterns. Here, we use a paradigm of repeated aggression and fighting deprivation to examine changes in behavior, neurogenesis, and neuronal activity in mice with positive fighting experience. We show that for males, repeated positive fighting experience induces persistent demonstration of aggression and stereotypic behaviors in daily agonistic interactions, enhances aggressive motivation, and elevates levels of anxiety. When winning males are deprived of opportunities to engage in further fights, they demonstrate increased levels of aggressiveness. Positive fighting experience results in increased levels of progenitor cell proliferation and production of young neurons in the hippocampus. This increase is not diminished after a fighting deprivation period. Furthermore, repeated winning experience decreases the number of activated (c-fos-positive) cells in the basolateral amygdala and increases the number of activated cells in the hippocampus; a subsequent no-fight period restores the number of c-fos-positive cells. Our results indicate that extended positive fighting experience in a social conflict heightens aggression, increases proliferation of neuronal progenitors and production of young neurons in the hippocampus, and decreases neuronal activity in the amygdala; these changes can be modified by depriving the winners of the opportunity for further fights.

No MeSH data available.


Related in: MedlinePlus

c-fos-Positive cells in the amygdala of male mice after 21 days of agonistic interactions. (A) c-fos cells in different regions of the amygdala of control and winning C57BL/Icg male mice: BLA, basolateral amygdala; BMA, basomedial amygdala; CEA, central amygdala. (B) c-fos cells in the basolateral amygdala (BLA) of C57BL/Icg male mice with different fighting experience: Win10, 10 day winners; Win21, 21 day winners; Win21-D, 21 day winners after 2 weeks of fighting deprivation. *P < 0.05 vs. the controls, +P < 0.01 vs. controls in BLA; t-test.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4664700&req=5

Figure 5: c-fos-Positive cells in the amygdala of male mice after 21 days of agonistic interactions. (A) c-fos cells in different regions of the amygdala of control and winning C57BL/Icg male mice: BLA, basolateral amygdala; BMA, basomedial amygdala; CEA, central amygdala. (B) c-fos cells in the basolateral amygdala (BLA) of C57BL/Icg male mice with different fighting experience: Win10, 10 day winners; Win21, 21 day winners; Win21-D, 21 day winners after 2 weeks of fighting deprivation. *P < 0.05 vs. the controls, +P < 0.01 vs. controls in BLA; t-test.

Mentions: Having detected changes in neuronal activity in the SGZ of the DG, we asked whether it is also altered in the amygdala. We first compared the number of c-fos cells in selected regions of the amygdala (BLA, BMA, and CeA) in control and Win21 males (Figure 5A). Two-way ANOVA with post-hoc t-test revealed the influence of factor “area” [F(2, 36) = 9.194; P < 0.001] and factor “groups” [control, winners; F(1, 36) = 6.809; P < 0.013]. t-Test revealed a significant decrease in the number of c-fos-positive cells in the BLA of the Win21 mice compared to the control animals (t = 2.84; P < 0.015). There was also a significant difference between the amygdala regions in the control animals (a smaller number of c-fos cells in the BMA (t = 3.31; P < 0.005) or CeA (t = 3.78; P < 0.002) than in BLA; factor “regions”), but not in the Win21 animals (factor “interactions”).


Altered Hippocampal Neurogenesis and Amygdalar Neuronal Activity in Adult Mice with Repeated Experience of Aggression.

Smagin DA, Park JH, Michurina TV, Peunova N, Glass Z, Sayed K, Bondar NP, Kovalenko IN, Kudryavtseva NN, Enikolopov G - Front Neurosci (2015)

c-fos-Positive cells in the amygdala of male mice after 21 days of agonistic interactions. (A) c-fos cells in different regions of the amygdala of control and winning C57BL/Icg male mice: BLA, basolateral amygdala; BMA, basomedial amygdala; CEA, central amygdala. (B) c-fos cells in the basolateral amygdala (BLA) of C57BL/Icg male mice with different fighting experience: Win10, 10 day winners; Win21, 21 day winners; Win21-D, 21 day winners after 2 weeks of fighting deprivation. *P < 0.05 vs. the controls, +P < 0.01 vs. controls in BLA; t-test.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4664700&req=5

Figure 5: c-fos-Positive cells in the amygdala of male mice after 21 days of agonistic interactions. (A) c-fos cells in different regions of the amygdala of control and winning C57BL/Icg male mice: BLA, basolateral amygdala; BMA, basomedial amygdala; CEA, central amygdala. (B) c-fos cells in the basolateral amygdala (BLA) of C57BL/Icg male mice with different fighting experience: Win10, 10 day winners; Win21, 21 day winners; Win21-D, 21 day winners after 2 weeks of fighting deprivation. *P < 0.05 vs. the controls, +P < 0.01 vs. controls in BLA; t-test.
Mentions: Having detected changes in neuronal activity in the SGZ of the DG, we asked whether it is also altered in the amygdala. We first compared the number of c-fos cells in selected regions of the amygdala (BLA, BMA, and CeA) in control and Win21 males (Figure 5A). Two-way ANOVA with post-hoc t-test revealed the influence of factor “area” [F(2, 36) = 9.194; P < 0.001] and factor “groups” [control, winners; F(1, 36) = 6.809; P < 0.013]. t-Test revealed a significant decrease in the number of c-fos-positive cells in the BLA of the Win21 mice compared to the control animals (t = 2.84; P < 0.015). There was also a significant difference between the amygdala regions in the control animals (a smaller number of c-fos cells in the BMA (t = 3.31; P < 0.005) or CeA (t = 3.78; P < 0.002) than in BLA; factor “regions”), but not in the Win21 animals (factor “interactions”).

Bottom Line: Positive fighting experience results in increased levels of progenitor cell proliferation and production of young neurons in the hippocampus.Furthermore, repeated winning experience decreases the number of activated (c-fos-positive) cells in the basolateral amygdala and increases the number of activated cells in the hippocampus; a subsequent no-fight period restores the number of c-fos-positive cells.Our results indicate that extended positive fighting experience in a social conflict heightens aggression, increases proliferation of neuronal progenitors and production of young neurons in the hippocampus, and decreases neuronal activity in the amygdala; these changes can be modified by depriving the winners of the opportunity for further fights.

View Article: PubMed Central - PubMed

Affiliation: Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences Novosibirsk, Russia ; Department of Nano-, Bio-, Information Technology and Cognitive Science, Moscow Institute of Physics and Technology Moscow, Russia ; Cold Spring Harbor Laboratory, Cold Spring Harbor NY, USA.

ABSTRACT
Repeated experience of winning in a social conflict setting elevates levels of aggression and may lead to violent behavioral patterns. Here, we use a paradigm of repeated aggression and fighting deprivation to examine changes in behavior, neurogenesis, and neuronal activity in mice with positive fighting experience. We show that for males, repeated positive fighting experience induces persistent demonstration of aggression and stereotypic behaviors in daily agonistic interactions, enhances aggressive motivation, and elevates levels of anxiety. When winning males are deprived of opportunities to engage in further fights, they demonstrate increased levels of aggressiveness. Positive fighting experience results in increased levels of progenitor cell proliferation and production of young neurons in the hippocampus. This increase is not diminished after a fighting deprivation period. Furthermore, repeated winning experience decreases the number of activated (c-fos-positive) cells in the basolateral amygdala and increases the number of activated cells in the hippocampus; a subsequent no-fight period restores the number of c-fos-positive cells. Our results indicate that extended positive fighting experience in a social conflict heightens aggression, increases proliferation of neuronal progenitors and production of young neurons in the hippocampus, and decreases neuronal activity in the amygdala; these changes can be modified by depriving the winners of the opportunity for further fights.

No MeSH data available.


Related in: MedlinePlus