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Effects of Floral Scents and Their Dietary Experiences on the Feeding Preference in the Blowfly, Phormia regina.

Maeda T, Tamotsu M, Yamaoka R, Ozaki M - Front Integr Neurosci (2015)

Bottom Line: After feeding on sucrose solutions flavored with floral scents for 5 days, the scents did not consistently show the previously observed effects.The results suggested that olfactory inputs through these organs play different roles in forming or modifying feeding preferences.Thus, our study contributes to an understanding of underlying mechanisms associated with the convergent processing of olfactory inputs with taste information, which affects feeding preference or appetite.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Graduate School of Science, Kobe University Kobe, Japan.

ABSTRACT
The flowers of different plant species have diverse scents with varied chemical compositions. Hence, every floral scent does not uniformly affect insect feeding preferences. The blowfly, Phormia regina, is a nectar feeder, and when a fly feeds on flower nectar, its olfactory organs, antennae, and maxillary palps are exposed to the scent. Generally, feeding preference is influenced by food flavor, which relies on both taste and odor. Therefore, the flies perceive the sweet taste of nectar and the particular scent of the flower simultaneously, and this olfactory information affects their feeding preference. Here, we show that the floral scents of 50 plant species have various effects on their sucrose feeding motivation, which was evaluated using the proboscis extension reflex (PER). Those floral scents were first categorized into three groups, based on their effects on the PER threshold sucrose concentration, which indicates whether a fly innately dislikes, ignores, or likes the target scent. Moreover, memory of olfactory experience with those floral scents during sugar feeding influenced the PER threshold. After feeding on sucrose solutions flavored with floral scents for 5 days, the scents did not consistently show the previously observed effects. Considering such empirical effects of scents on the PER threshold, we categorized the effects of the 50 tested floral scents on feeding preference into 16 of all possible 27 theoretical types. We then conducted the same experiments with flies whose antennae or maxillary palps were ablated prior to PER test in a fly group naïve to floral scents and prior to the olfactory experience during sugar feeding in the other fly group in order to test how these organs were involved in the effect of the floral scent. The results suggested that olfactory inputs through these organs play different roles in forming or modifying feeding preferences. Thus, our study contributes to an understanding of underlying mechanisms associated with the convergent processing of olfactory inputs with taste information, which affects feeding preference or appetite.

No MeSH data available.


Related in: MedlinePlus

Effects of olfactory organ ablation on the sucrose concentration-PER curves modified by Narcissus tazetta floral scent. (I) Olfactory organ ablation was carried out prior to the PER tests and its effects are compared among curves in non-experienced flies (open circles); (II) Olfactory organ ablation was carried out prior to the olfactory experience and its effects are compared among curves in the experienced flies (closed circles). Top: The curves in the absence (black circles) and presence (red circles) of scent in the intact flies. Middle: The curves in the absence and presence of scent in the antennae-ablated flies. Bottom: The curves in the absence and presence of scent in the maxillary-palp-ablated flies. The black and red arrowheads indicate the mean PER threshold sucrose concentrations in the absence and presence of scent, respectively. Broken lines indicate the curves in the absence of scent in the non-experienced flies.
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Figure 6: Effects of olfactory organ ablation on the sucrose concentration-PER curves modified by Narcissus tazetta floral scent. (I) Olfactory organ ablation was carried out prior to the PER tests and its effects are compared among curves in non-experienced flies (open circles); (II) Olfactory organ ablation was carried out prior to the olfactory experience and its effects are compared among curves in the experienced flies (closed circles). Top: The curves in the absence (black circles) and presence (red circles) of scent in the intact flies. Middle: The curves in the absence and presence of scent in the antennae-ablated flies. Bottom: The curves in the absence and presence of scent in the maxillary-palp-ablated flies. The black and red arrowheads indicate the mean PER threshold sucrose concentrations in the absence and presence of scent, respectively. Broken lines indicate the curves in the absence of scent in the non-experienced flies.

Mentions: Figure 6I shows the appetite change of flies when either the antennae or maxillary palps were ablated prior to PER tests in non-experienced flies. When the antennae were ablated and the maxillary palps were preserved, fly appetites in the presence of the N. tazetta floral scent increased, and the individual PER decreased significantly (p < 0.05, Mann-Whitney U test; n = 20). The mean PER threshold (0.261 M) then decreased to about a quarter of that observed in the absence of scent (0.997 M). When the maxillary palps were ablated and the antennae were preserved, the fly appetites in the presence of scent decreased, and the individual PER increased significantly (p < 0.05, Mann-Whitney U test; n = 20). The mean PER threshold then increased approximately three-fold (2.166 M) compared to that observed in the absence of scent (0.800 M).


Effects of Floral Scents and Their Dietary Experiences on the Feeding Preference in the Blowfly, Phormia regina.

Maeda T, Tamotsu M, Yamaoka R, Ozaki M - Front Integr Neurosci (2015)

Effects of olfactory organ ablation on the sucrose concentration-PER curves modified by Narcissus tazetta floral scent. (I) Olfactory organ ablation was carried out prior to the PER tests and its effects are compared among curves in non-experienced flies (open circles); (II) Olfactory organ ablation was carried out prior to the olfactory experience and its effects are compared among curves in the experienced flies (closed circles). Top: The curves in the absence (black circles) and presence (red circles) of scent in the intact flies. Middle: The curves in the absence and presence of scent in the antennae-ablated flies. Bottom: The curves in the absence and presence of scent in the maxillary-palp-ablated flies. The black and red arrowheads indicate the mean PER threshold sucrose concentrations in the absence and presence of scent, respectively. Broken lines indicate the curves in the absence of scent in the non-experienced flies.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4664696&req=5

Figure 6: Effects of olfactory organ ablation on the sucrose concentration-PER curves modified by Narcissus tazetta floral scent. (I) Olfactory organ ablation was carried out prior to the PER tests and its effects are compared among curves in non-experienced flies (open circles); (II) Olfactory organ ablation was carried out prior to the olfactory experience and its effects are compared among curves in the experienced flies (closed circles). Top: The curves in the absence (black circles) and presence (red circles) of scent in the intact flies. Middle: The curves in the absence and presence of scent in the antennae-ablated flies. Bottom: The curves in the absence and presence of scent in the maxillary-palp-ablated flies. The black and red arrowheads indicate the mean PER threshold sucrose concentrations in the absence and presence of scent, respectively. Broken lines indicate the curves in the absence of scent in the non-experienced flies.
Mentions: Figure 6I shows the appetite change of flies when either the antennae or maxillary palps were ablated prior to PER tests in non-experienced flies. When the antennae were ablated and the maxillary palps were preserved, fly appetites in the presence of the N. tazetta floral scent increased, and the individual PER decreased significantly (p < 0.05, Mann-Whitney U test; n = 20). The mean PER threshold (0.261 M) then decreased to about a quarter of that observed in the absence of scent (0.997 M). When the maxillary palps were ablated and the antennae were preserved, the fly appetites in the presence of scent decreased, and the individual PER increased significantly (p < 0.05, Mann-Whitney U test; n = 20). The mean PER threshold then increased approximately three-fold (2.166 M) compared to that observed in the absence of scent (0.800 M).

Bottom Line: After feeding on sucrose solutions flavored with floral scents for 5 days, the scents did not consistently show the previously observed effects.The results suggested that olfactory inputs through these organs play different roles in forming or modifying feeding preferences.Thus, our study contributes to an understanding of underlying mechanisms associated with the convergent processing of olfactory inputs with taste information, which affects feeding preference or appetite.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Graduate School of Science, Kobe University Kobe, Japan.

ABSTRACT
The flowers of different plant species have diverse scents with varied chemical compositions. Hence, every floral scent does not uniformly affect insect feeding preferences. The blowfly, Phormia regina, is a nectar feeder, and when a fly feeds on flower nectar, its olfactory organs, antennae, and maxillary palps are exposed to the scent. Generally, feeding preference is influenced by food flavor, which relies on both taste and odor. Therefore, the flies perceive the sweet taste of nectar and the particular scent of the flower simultaneously, and this olfactory information affects their feeding preference. Here, we show that the floral scents of 50 plant species have various effects on their sucrose feeding motivation, which was evaluated using the proboscis extension reflex (PER). Those floral scents were first categorized into three groups, based on their effects on the PER threshold sucrose concentration, which indicates whether a fly innately dislikes, ignores, or likes the target scent. Moreover, memory of olfactory experience with those floral scents during sugar feeding influenced the PER threshold. After feeding on sucrose solutions flavored with floral scents for 5 days, the scents did not consistently show the previously observed effects. Considering such empirical effects of scents on the PER threshold, we categorized the effects of the 50 tested floral scents on feeding preference into 16 of all possible 27 theoretical types. We then conducted the same experiments with flies whose antennae or maxillary palps were ablated prior to PER test in a fly group naïve to floral scents and prior to the olfactory experience during sugar feeding in the other fly group in order to test how these organs were involved in the effect of the floral scent. The results suggested that olfactory inputs through these organs play different roles in forming or modifying feeding preferences. Thus, our study contributes to an understanding of underlying mechanisms associated with the convergent processing of olfactory inputs with taste information, which affects feeding preference or appetite.

No MeSH data available.


Related in: MedlinePlus