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miRNA profiling in autism spectrum disorder in China.

Huang F, Zhou T, Yao X, Yi J, Zhou F, Long Z, Hou X, Wang C, Chen Z, Jiang H - Genom Data (2015)

Bottom Line: It is characterized by impaired social abilities, disordered language, isolated areas of interest, and repetitive behaviors.Evidence suggested that the neuropathology of ASD is widely distributed, involving epigenetic regulation in the brain.MiRNAs are a group of endogenous non-coding RNAs that play a critical role in neurodevelopment, neuroplasticity, and other fundamental neurobiological processes.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology & Institute of Translational Medicine at University of South China, The First People's Hospital of Chenzhou, Chenzhou, Hunan 423000, PR China.

ABSTRACT
Autism spectrum disorder (ASD) is a clinically complex and heterogeneous disorder. It is characterized by impaired social abilities, disordered language, isolated areas of interest, and repetitive behaviors. Evidence suggested that the neuropathology of ASD is widely distributed, involving epigenetic regulation in the brain. MiRNAs are a group of endogenous non-coding RNAs that play a critical role in neurodevelopment, neuroplasticity, and other fundamental neurobiological processes. To study miRNA profiling in Autism spectrum disorder in China, we performed miRNA microarray followed quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Here, we describe detailed methods and analysis on these microarray data which has been deposited in Gene Expression Omnibus (GEO): GSE67979.

No MeSH data available.


Related in: MedlinePlus

Box plot of raw data.Note: C1-5 means controls, A1-5 means patients.
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f0010: Box plot of raw data.Note: C1-5 means controls, A1-5 means patients.

Mentions: RiboArray™ miDETECT™ Human Array microarrays (RIBOBIO), which contained 2578 assay probes corresponding to the entire set of primate miRNAs, were used to screen the miRNA expression. Microarray was incubated 10 min in 65 °C followed by 1 h in 37 °C for prehybridization. 2.5 μg total RNA of each sample was labeled by Cy5. The Cy5-labeled RNA samples could be readily visualized with comparable intensity under UV. Labeling efficiency (1.0 to 3.6 was accepted for good microarray result) can be calculated by the concentration of CyDye and RNA measured by K5500 micro-spectrophotometer. Cy5-labeled RNA was denaturated 3 min in hybridization solution, incubated 20 s on ice, and hybridized to microarray 16 h in 37 °C. All microarrays were successively washed by 6 × SSPET, 3 × SSPET, 0.5 × SSPET, and 0.5 × SSPET. Then, developer and coverslip were plus for scan (GenePix 4000B laser scanner), followed by bioinformatic analysis (digitized using the R software). Fig. 1 showed part of the signal collection. Fig. 2 showed the log2 scale of the expression signal values that were plotted, including control probes.


miRNA profiling in autism spectrum disorder in China.

Huang F, Zhou T, Yao X, Yi J, Zhou F, Long Z, Hou X, Wang C, Chen Z, Jiang H - Genom Data (2015)

Box plot of raw data.Note: C1-5 means controls, A1-5 means patients.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4664689&req=5

f0010: Box plot of raw data.Note: C1-5 means controls, A1-5 means patients.
Mentions: RiboArray™ miDETECT™ Human Array microarrays (RIBOBIO), which contained 2578 assay probes corresponding to the entire set of primate miRNAs, were used to screen the miRNA expression. Microarray was incubated 10 min in 65 °C followed by 1 h in 37 °C for prehybridization. 2.5 μg total RNA of each sample was labeled by Cy5. The Cy5-labeled RNA samples could be readily visualized with comparable intensity under UV. Labeling efficiency (1.0 to 3.6 was accepted for good microarray result) can be calculated by the concentration of CyDye and RNA measured by K5500 micro-spectrophotometer. Cy5-labeled RNA was denaturated 3 min in hybridization solution, incubated 20 s on ice, and hybridized to microarray 16 h in 37 °C. All microarrays were successively washed by 6 × SSPET, 3 × SSPET, 0.5 × SSPET, and 0.5 × SSPET. Then, developer and coverslip were plus for scan (GenePix 4000B laser scanner), followed by bioinformatic analysis (digitized using the R software). Fig. 1 showed part of the signal collection. Fig. 2 showed the log2 scale of the expression signal values that were plotted, including control probes.

Bottom Line: It is characterized by impaired social abilities, disordered language, isolated areas of interest, and repetitive behaviors.Evidence suggested that the neuropathology of ASD is widely distributed, involving epigenetic regulation in the brain.MiRNAs are a group of endogenous non-coding RNAs that play a critical role in neurodevelopment, neuroplasticity, and other fundamental neurobiological processes.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology & Institute of Translational Medicine at University of South China, The First People's Hospital of Chenzhou, Chenzhou, Hunan 423000, PR China.

ABSTRACT
Autism spectrum disorder (ASD) is a clinically complex and heterogeneous disorder. It is characterized by impaired social abilities, disordered language, isolated areas of interest, and repetitive behaviors. Evidence suggested that the neuropathology of ASD is widely distributed, involving epigenetic regulation in the brain. MiRNAs are a group of endogenous non-coding RNAs that play a critical role in neurodevelopment, neuroplasticity, and other fundamental neurobiological processes. To study miRNA profiling in Autism spectrum disorder in China, we performed miRNA microarray followed quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Here, we describe detailed methods and analysis on these microarray data which has been deposited in Gene Expression Omnibus (GEO): GSE67979.

No MeSH data available.


Related in: MedlinePlus