Limits...
Genome-wide profiling to analyze the effects of FXR activation on mouse renal proximal tubular cells.

Gui T, Gai Z - Genom Data (2015)

Bottom Line: Analysis of gene expression in the proximal tubular cells by whole genome microarrays indicated that FXR activation induced genes involved in fatty acid degradation and oxidation reduction.Among them, genes involved in glutathione metabolism were mostly induced.Here we describe in details the contents and quality controls for the gene expression and related results associated with the data uploaded to Gene Expression Omnibus (accession number GSE70296).

View Article: PubMed Central - PubMed

Affiliation: Department of Nephrology, Hypertension and Clinical Pharmacology, Inselspital, 3010 Bern, Switzerland.

ABSTRACT
To assess the effect of farnesoid X receptor (FXR), a bile acid nuclear receptor, on renal proximal tubular cells, primary cultured mouse kidney proximal tubular cells were treated with GW4064 (a FXR agonist) or DMSO (as controls) overnight. Analysis of gene expression in the proximal tubular cells by whole genome microarrays indicated that FXR activation induced genes involved in fatty acid degradation and oxidation reduction. Among them, genes involved in glutathione metabolism were mostly induced. Here we describe in details the contents and quality controls for the gene expression and related results associated with the data uploaded to Gene Expression Omnibus (accession number GSE70296).

No MeSH data available.


Related in: MedlinePlus

Comparison of distinct gene expression patterns between the different experimental conditions. (A) Summary of differentially expressed genes between GW4064 treatment overnight and vehicle control (DMSO). Genes that are differentially increased or decreased after GW4064 treatment are indicated by orange and green bars, respectively. Cut-off 1.7-fold, p < 0.05. Of all 550 genes significantly expressed after GW4064 treatment, 357 genes are increased and 193 genes are reduced. (B) KEGG pathway analysis of genes with a change in expression of ≥ 1.7 fold increase (orange bars).
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4664674&req=5

f0005: Comparison of distinct gene expression patterns between the different experimental conditions. (A) Summary of differentially expressed genes between GW4064 treatment overnight and vehicle control (DMSO). Genes that are differentially increased or decreased after GW4064 treatment are indicated by orange and green bars, respectively. Cut-off 1.7-fold, p < 0.05. Of all 550 genes significantly expressed after GW4064 treatment, 357 genes are increased and 193 genes are reduced. (B) KEGG pathway analysis of genes with a change in expression of ≥ 1.7 fold increase (orange bars).

Mentions: First, gene expression was compared between GW4064-treated cells versus DMSO-treated cells. By using a ≥ 1.7-fold change as a cut-off, 550 genes were significantly expressed by FXR activation. Among them, 193 genes were down-regulated and 357 genes were up-regulated (Fig. 1A). Interestingly, by KEGG pathway analysis, several pathways involved in fatty acid metabolism and oxidation reduction were induced by FXR activation. Pathways belonging to oxidation reduction include glutathione metabolism pathway, drug metabolism pathway and xenobiotic metabolism pathway (Fig. 1B). Furthermore, by comparison between public available ChIP-seq data (GSE57305, altered mRNA expression profile of GW4064 treated mouse livers compared to vehicle control, http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE57305) with our microarray data (GSE70296, effect of GW4064 on primary cultured mouse kidney proximal tubule cells), we identified that activated FXR could regulate similar pathways in mouse liver in vivo as the pathways in mouse proximal tubular cells (such as glutathione metabolism pathway, drug metabolism pathway, fatty acid metabolism pathway and pyruvate metabolic process pathway), indicating a universal role of FXR in both mouse kidney and liver (Table 1).


Genome-wide profiling to analyze the effects of FXR activation on mouse renal proximal tubular cells.

Gui T, Gai Z - Genom Data (2015)

Comparison of distinct gene expression patterns between the different experimental conditions. (A) Summary of differentially expressed genes between GW4064 treatment overnight and vehicle control (DMSO). Genes that are differentially increased or decreased after GW4064 treatment are indicated by orange and green bars, respectively. Cut-off 1.7-fold, p < 0.05. Of all 550 genes significantly expressed after GW4064 treatment, 357 genes are increased and 193 genes are reduced. (B) KEGG pathway analysis of genes with a change in expression of ≥ 1.7 fold increase (orange bars).
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4664674&req=5

f0005: Comparison of distinct gene expression patterns between the different experimental conditions. (A) Summary of differentially expressed genes between GW4064 treatment overnight and vehicle control (DMSO). Genes that are differentially increased or decreased after GW4064 treatment are indicated by orange and green bars, respectively. Cut-off 1.7-fold, p < 0.05. Of all 550 genes significantly expressed after GW4064 treatment, 357 genes are increased and 193 genes are reduced. (B) KEGG pathway analysis of genes with a change in expression of ≥ 1.7 fold increase (orange bars).
Mentions: First, gene expression was compared between GW4064-treated cells versus DMSO-treated cells. By using a ≥ 1.7-fold change as a cut-off, 550 genes were significantly expressed by FXR activation. Among them, 193 genes were down-regulated and 357 genes were up-regulated (Fig. 1A). Interestingly, by KEGG pathway analysis, several pathways involved in fatty acid metabolism and oxidation reduction were induced by FXR activation. Pathways belonging to oxidation reduction include glutathione metabolism pathway, drug metabolism pathway and xenobiotic metabolism pathway (Fig. 1B). Furthermore, by comparison between public available ChIP-seq data (GSE57305, altered mRNA expression profile of GW4064 treated mouse livers compared to vehicle control, http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE57305) with our microarray data (GSE70296, effect of GW4064 on primary cultured mouse kidney proximal tubule cells), we identified that activated FXR could regulate similar pathways in mouse liver in vivo as the pathways in mouse proximal tubular cells (such as glutathione metabolism pathway, drug metabolism pathway, fatty acid metabolism pathway and pyruvate metabolic process pathway), indicating a universal role of FXR in both mouse kidney and liver (Table 1).

Bottom Line: Analysis of gene expression in the proximal tubular cells by whole genome microarrays indicated that FXR activation induced genes involved in fatty acid degradation and oxidation reduction.Among them, genes involved in glutathione metabolism were mostly induced.Here we describe in details the contents and quality controls for the gene expression and related results associated with the data uploaded to Gene Expression Omnibus (accession number GSE70296).

View Article: PubMed Central - PubMed

Affiliation: Department of Nephrology, Hypertension and Clinical Pharmacology, Inselspital, 3010 Bern, Switzerland.

ABSTRACT
To assess the effect of farnesoid X receptor (FXR), a bile acid nuclear receptor, on renal proximal tubular cells, primary cultured mouse kidney proximal tubular cells were treated with GW4064 (a FXR agonist) or DMSO (as controls) overnight. Analysis of gene expression in the proximal tubular cells by whole genome microarrays indicated that FXR activation induced genes involved in fatty acid degradation and oxidation reduction. Among them, genes involved in glutathione metabolism were mostly induced. Here we describe in details the contents and quality controls for the gene expression and related results associated with the data uploaded to Gene Expression Omnibus (accession number GSE70296).

No MeSH data available.


Related in: MedlinePlus