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Microarray analyses during early and later stages of the Arabidopsis/Piriformospora indica interaction.

Vahabi K, Sherameti I, Bakshi M, Mrozinska A, Ludwig A, Oelmüller R - Genom Data (2015)

Bottom Line: An increase of the biomass and seed yield is other beneficial effect of P. indica for the host plants.We describe a co-cultivation system which allows us to investigate the effects of fungal exudates on the root transcriptome before and after the establishment of a physical contact, and during early phases of root colonization.We present a detailed protocol which facilitates easy reproduction of the results (NCBI GEO accession number GSE58771) published by Vahabi et al. (2015) in BMC Plant Biology [1].

View Article: PubMed Central - PubMed

Affiliation: Institute of General Botany and Plant Physiology, Friedrich-Schiller-University Jena, Dornburger Str. 159, 07743 Jena, Germany.

ABSTRACT
Colonization of the roots of different plant species by Piriformospora indica results in better plant performance and biotic and abiotic stress tolerance. An increase of the biomass and seed yield is other beneficial effect of P. indica for the host plants. The interaction of P. indica with Arabidopsis thaliana roots is a unique model system to study symbiotic relationships. We describe a co-cultivation system which allows us to investigate the effects of fungal exudates on the root transcriptome before and after the establishment of a physical contact, and during early phases of root colonization. We present a detailed protocol which facilitates easy reproduction of the results (NCBI GEO accession number GSE58771) published by Vahabi et al. (2015) in BMC Plant Biology [1].

No MeSH data available.


A. thaliana co-cultivation with P. indica (A, C, D) or mock-treated (B, E, F) after 2 days. Visible light microscopic view of A. thaliana root stained with trypan blue (C, D, E, F) after 2 days of co-cultivation with P. indica (C, D) or mock-treated (E, F). The roots were stained with trypan blue.
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f0005: A. thaliana co-cultivation with P. indica (A, C, D) or mock-treated (B, E, F) after 2 days. Visible light microscopic view of A. thaliana root stained with trypan blue (C, D, E, F) after 2 days of co-cultivation with P. indica (C, D) or mock-treated (E, F). The roots were stained with trypan blue.

Mentions: Three seedlings (equal in size and number of leaves) from twelve days-old plants (described above) were picked from the MS plates and their roots were laid onto the surface of a nylon membrane in a distance of 3 cm from the plaques. All plates were transferred to 22 °C under continuous illumination (80 ± 5 μmol m− 2 s− 1) from the top (distance from light source = 30 cm) for 2 or 6 days. While no physical contact has been established after 2 days of co-cultivation, microscopic staining and PCR analyses confirmed the presence of fungal mycelium in and around the roots after 6 days of co-cultivation (Fig. 1, Fig. 2).


Microarray analyses during early and later stages of the Arabidopsis/Piriformospora indica interaction.

Vahabi K, Sherameti I, Bakshi M, Mrozinska A, Ludwig A, Oelmüller R - Genom Data (2015)

A. thaliana co-cultivation with P. indica (A, C, D) or mock-treated (B, E, F) after 2 days. Visible light microscopic view of A. thaliana root stained with trypan blue (C, D, E, F) after 2 days of co-cultivation with P. indica (C, D) or mock-treated (E, F). The roots were stained with trypan blue.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4664658&req=5

f0005: A. thaliana co-cultivation with P. indica (A, C, D) or mock-treated (B, E, F) after 2 days. Visible light microscopic view of A. thaliana root stained with trypan blue (C, D, E, F) after 2 days of co-cultivation with P. indica (C, D) or mock-treated (E, F). The roots were stained with trypan blue.
Mentions: Three seedlings (equal in size and number of leaves) from twelve days-old plants (described above) were picked from the MS plates and their roots were laid onto the surface of a nylon membrane in a distance of 3 cm from the plaques. All plates were transferred to 22 °C under continuous illumination (80 ± 5 μmol m− 2 s− 1) from the top (distance from light source = 30 cm) for 2 or 6 days. While no physical contact has been established after 2 days of co-cultivation, microscopic staining and PCR analyses confirmed the presence of fungal mycelium in and around the roots after 6 days of co-cultivation (Fig. 1, Fig. 2).

Bottom Line: An increase of the biomass and seed yield is other beneficial effect of P. indica for the host plants.We describe a co-cultivation system which allows us to investigate the effects of fungal exudates on the root transcriptome before and after the establishment of a physical contact, and during early phases of root colonization.We present a detailed protocol which facilitates easy reproduction of the results (NCBI GEO accession number GSE58771) published by Vahabi et al. (2015) in BMC Plant Biology [1].

View Article: PubMed Central - PubMed

Affiliation: Institute of General Botany and Plant Physiology, Friedrich-Schiller-University Jena, Dornburger Str. 159, 07743 Jena, Germany.

ABSTRACT
Colonization of the roots of different plant species by Piriformospora indica results in better plant performance and biotic and abiotic stress tolerance. An increase of the biomass and seed yield is other beneficial effect of P. indica for the host plants. The interaction of P. indica with Arabidopsis thaliana roots is a unique model system to study symbiotic relationships. We describe a co-cultivation system which allows us to investigate the effects of fungal exudates on the root transcriptome before and after the establishment of a physical contact, and during early phases of root colonization. We present a detailed protocol which facilitates easy reproduction of the results (NCBI GEO accession number GSE58771) published by Vahabi et al. (2015) in BMC Plant Biology [1].

No MeSH data available.