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Loss of Drp1 in the liver leads to an alteration in expression of the genes involved in the immune system.

Wang L, Nomura M - Genom Data (2015)

Bottom Line: Dynamin-related protein 1 (Drp1) is a member of the dynamin family of large GTPase, which cycles between the cytosol and the mitochondrial outer membrane, and mediates mitochondrial fission.Using microarray analysis of gene expression in the livers of wild-type and Drp1 knockout mice, we have previously identified that endoplasmic reticulum (ER) stress marker genes are significantly increased by the absence of Drp1 [1].Here, we provide methodological and analytical details of the microarray data, which have been deposited in the Gene Expression Omnibus as data set GSE64222.

View Article: PubMed Central - PubMed

Affiliation: Department of Medicine and Bioregulatory Science, Graduate School of Medical Science, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka 812-8582, Japan.

ABSTRACT
Dynamin-related protein 1 (Drp1) is a member of the dynamin family of large GTPase, which cycles between the cytosol and the mitochondrial outer membrane, and mediates mitochondrial fission. Using microarray analysis of gene expression in the livers of wild-type and Drp1 knockout mice, we have previously identified that endoplasmic reticulum (ER) stress marker genes are significantly increased by the absence of Drp1 [1]. Here, we provide methodological and analytical details of the microarray data, which have been deposited in the Gene Expression Omnibus as data set GSE64222. We have performed further gene ontology analysis of the data and found the differential expression of a subset of genes that are involved in the immune response in the livers of Drp1 knockout mice versus wild-type controls.

No MeSH data available.


Related in: MedlinePlus

Microarray functional annotation summary results of the down-regulated genes.
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f0010: Microarray functional annotation summary results of the down-regulated genes.

Mentions: Genes were selected using the criterion of a Z score of ≥ 2, which identified 526 up-regulated and 640 down-regulated genes in Drp1LiKO mice. The top 5 up-regulated genes have been reported in other publications, of these, 3 genes are known to be ER stress response genes, which was the topic of our previously published article [1]. To further investigate these data, we use the DAVID tool to functionally cluster up-regulated and down-regulated genes by similarly annotated gene ontology (GO) biological process terms, respectively. The top ten significantly enriched annotation clusters of up-regulated genes were shown in Fig. 1. We found that seven of the ten clusters were related to the immune system; these clusters included terms such as immune response, phagocytosis, antigen processing and presentation, defense response and response to virus. The remaining clusters were related to 2′-deoxyribonucleotide biosynthetic process, amine biosynthetic process and cell death. Next, to further define connections between immune molecules that were regulated by mitochondrial fission, we list the 32 genes selected by using the term “immune response” in Table 1. The most significant ten clusters for down-regulated genes were presented in Fig. 2, and include lipid biosynthetic process, fatty acid metabolic process, acute inflammatory response, actin filament-based process, cell fate commitment, response to wounding, actomyosin structure organization, complement activation, alternative pathway, actin cytoskeleton organization and brown fat cell differentiation.


Loss of Drp1 in the liver leads to an alteration in expression of the genes involved in the immune system.

Wang L, Nomura M - Genom Data (2015)

Microarray functional annotation summary results of the down-regulated genes.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4664657&req=5

f0010: Microarray functional annotation summary results of the down-regulated genes.
Mentions: Genes were selected using the criterion of a Z score of ≥ 2, which identified 526 up-regulated and 640 down-regulated genes in Drp1LiKO mice. The top 5 up-regulated genes have been reported in other publications, of these, 3 genes are known to be ER stress response genes, which was the topic of our previously published article [1]. To further investigate these data, we use the DAVID tool to functionally cluster up-regulated and down-regulated genes by similarly annotated gene ontology (GO) biological process terms, respectively. The top ten significantly enriched annotation clusters of up-regulated genes were shown in Fig. 1. We found that seven of the ten clusters were related to the immune system; these clusters included terms such as immune response, phagocytosis, antigen processing and presentation, defense response and response to virus. The remaining clusters were related to 2′-deoxyribonucleotide biosynthetic process, amine biosynthetic process and cell death. Next, to further define connections between immune molecules that were regulated by mitochondrial fission, we list the 32 genes selected by using the term “immune response” in Table 1. The most significant ten clusters for down-regulated genes were presented in Fig. 2, and include lipid biosynthetic process, fatty acid metabolic process, acute inflammatory response, actin filament-based process, cell fate commitment, response to wounding, actomyosin structure organization, complement activation, alternative pathway, actin cytoskeleton organization and brown fat cell differentiation.

Bottom Line: Dynamin-related protein 1 (Drp1) is a member of the dynamin family of large GTPase, which cycles between the cytosol and the mitochondrial outer membrane, and mediates mitochondrial fission.Using microarray analysis of gene expression in the livers of wild-type and Drp1 knockout mice, we have previously identified that endoplasmic reticulum (ER) stress marker genes are significantly increased by the absence of Drp1 [1].Here, we provide methodological and analytical details of the microarray data, which have been deposited in the Gene Expression Omnibus as data set GSE64222.

View Article: PubMed Central - PubMed

Affiliation: Department of Medicine and Bioregulatory Science, Graduate School of Medical Science, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka 812-8582, Japan.

ABSTRACT
Dynamin-related protein 1 (Drp1) is a member of the dynamin family of large GTPase, which cycles between the cytosol and the mitochondrial outer membrane, and mediates mitochondrial fission. Using microarray analysis of gene expression in the livers of wild-type and Drp1 knockout mice, we have previously identified that endoplasmic reticulum (ER) stress marker genes are significantly increased by the absence of Drp1 [1]. Here, we provide methodological and analytical details of the microarray data, which have been deposited in the Gene Expression Omnibus as data set GSE64222. We have performed further gene ontology analysis of the data and found the differential expression of a subset of genes that are involved in the immune response in the livers of Drp1 knockout mice versus wild-type controls.

No MeSH data available.


Related in: MedlinePlus