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SNF3 as High Affinity Glucose Sensor and Its Function in Supporting the Viability of Candida glabrata under Glucose-Limited Environment.

Ng TS, Chew SY, Rangasamy P, Mohd Desa MN, Sandai D, Chong PP, Than LT - Front Microbiol (2015)

Bottom Line: Candida glabrata is an emerging human fungal pathogen that has efficacious nutrient sensing and responsiveness ability.It can be seen through its ability to thrive in diverse range of nutrient limited-human anatomical sites.The deletion of SNF3 also resulted in the down-regulation of about half of hexose transporters genes (four out of nine).

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia Serdang, Malaysia.

ABSTRACT
Candida glabrata is an emerging human fungal pathogen that has efficacious nutrient sensing and responsiveness ability. It can be seen through its ability to thrive in diverse range of nutrient limited-human anatomical sites. Therefore, nutrient sensing particularly glucose sensing is thought to be crucial in contributing to the development and fitness of the pathogen. This study aimed to elucidate the role of SNF3 (Sucrose Non Fermenting 3) as a glucose sensor and its possible role in contributing to the fitness and survivability of C. glabrata in glucose-limited environment. The SNF3 knockout strain was constructed and subjected to different glucose concentrations to evaluate its growth, biofilm formation, amphotericin B susceptibility, ex vivo survivability and effects on the transcriptional profiling of the sugar receptor repressor (SRR) pathway-related genes. The CgSNF3Δ strain showed a retarded growth in low glucose environments (0.01 and 0.1%) in both fermentation and respiration-preferred conditions but grew well in high glucose concentration environments (1 and 2%). It was also found to be more susceptible to amphotericin B in low glucose environment (0.1%) and macrophage engulfment but showed no difference in the biofilm formation capability. The deletion of SNF3 also resulted in the down-regulation of about half of hexose transporters genes (four out of nine). Overall, the deletion of SNF3 causes significant reduction in the ability of C. glabrata to sense limited surrounding glucose and consequently disrupts its competency to transport and perform the uptake of this critical nutrient. This study highlighted the role of SNF3 as a high affinity glucose sensor and its role in aiding the survivability of C. glabrata particularly in glucose limited environment.

No MeSH data available.


Related in: MedlinePlus

The survival ratio of Candida glabrata BG2 and SNF3Δ strains recovered from macrophages at 24 h vs. 2 h after co-cultivation. Unpaired T-test was carried out for the statistical analysis to examine the significant differences (indicated by *) between BG2 and SNF3Δ (p-value < 0.01).
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Figure 6: The survival ratio of Candida glabrata BG2 and SNF3Δ strains recovered from macrophages at 24 h vs. 2 h after co-cultivation. Unpaired T-test was carried out for the statistical analysis to examine the significant differences (indicated by *) between BG2 and SNF3Δ (p-value < 0.01).

Mentions: The microenvironment in macrophage is always linked to nutrient-limited environment, particularly in glucose availability. In order to validate the possible role of SNF3 in promoting the fitness of C. glabrata under glucose-limited environment, the survivability of macrophage trapped- C. glabrata was assayed in an ex vivo manner. Results demonstrated a significant reduced growth (p-value < 0.01) of the internalized mutant strain in comparison to wild type strain (Figure 6) and this suggests the essential role of SNF3 in supporting the survivability of C. glabrata upon macrophage engulfment.


SNF3 as High Affinity Glucose Sensor and Its Function in Supporting the Viability of Candida glabrata under Glucose-Limited Environment.

Ng TS, Chew SY, Rangasamy P, Mohd Desa MN, Sandai D, Chong PP, Than LT - Front Microbiol (2015)

The survival ratio of Candida glabrata BG2 and SNF3Δ strains recovered from macrophages at 24 h vs. 2 h after co-cultivation. Unpaired T-test was carried out for the statistical analysis to examine the significant differences (indicated by *) between BG2 and SNF3Δ (p-value < 0.01).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4664639&req=5

Figure 6: The survival ratio of Candida glabrata BG2 and SNF3Δ strains recovered from macrophages at 24 h vs. 2 h after co-cultivation. Unpaired T-test was carried out for the statistical analysis to examine the significant differences (indicated by *) between BG2 and SNF3Δ (p-value < 0.01).
Mentions: The microenvironment in macrophage is always linked to nutrient-limited environment, particularly in glucose availability. In order to validate the possible role of SNF3 in promoting the fitness of C. glabrata under glucose-limited environment, the survivability of macrophage trapped- C. glabrata was assayed in an ex vivo manner. Results demonstrated a significant reduced growth (p-value < 0.01) of the internalized mutant strain in comparison to wild type strain (Figure 6) and this suggests the essential role of SNF3 in supporting the survivability of C. glabrata upon macrophage engulfment.

Bottom Line: Candida glabrata is an emerging human fungal pathogen that has efficacious nutrient sensing and responsiveness ability.It can be seen through its ability to thrive in diverse range of nutrient limited-human anatomical sites.The deletion of SNF3 also resulted in the down-regulation of about half of hexose transporters genes (four out of nine).

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia Serdang, Malaysia.

ABSTRACT
Candida glabrata is an emerging human fungal pathogen that has efficacious nutrient sensing and responsiveness ability. It can be seen through its ability to thrive in diverse range of nutrient limited-human anatomical sites. Therefore, nutrient sensing particularly glucose sensing is thought to be crucial in contributing to the development and fitness of the pathogen. This study aimed to elucidate the role of SNF3 (Sucrose Non Fermenting 3) as a glucose sensor and its possible role in contributing to the fitness and survivability of C. glabrata in glucose-limited environment. The SNF3 knockout strain was constructed and subjected to different glucose concentrations to evaluate its growth, biofilm formation, amphotericin B susceptibility, ex vivo survivability and effects on the transcriptional profiling of the sugar receptor repressor (SRR) pathway-related genes. The CgSNF3Δ strain showed a retarded growth in low glucose environments (0.01 and 0.1%) in both fermentation and respiration-preferred conditions but grew well in high glucose concentration environments (1 and 2%). It was also found to be more susceptible to amphotericin B in low glucose environment (0.1%) and macrophage engulfment but showed no difference in the biofilm formation capability. The deletion of SNF3 also resulted in the down-regulation of about half of hexose transporters genes (four out of nine). Overall, the deletion of SNF3 causes significant reduction in the ability of C. glabrata to sense limited surrounding glucose and consequently disrupts its competency to transport and perform the uptake of this critical nutrient. This study highlighted the role of SNF3 as a high affinity glucose sensor and its role in aiding the survivability of C. glabrata particularly in glucose limited environment.

No MeSH data available.


Related in: MedlinePlus