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SNF3 as High Affinity Glucose Sensor and Its Function in Supporting the Viability of Candida glabrata under Glucose-Limited Environment.

Ng TS, Chew SY, Rangasamy P, Mohd Desa MN, Sandai D, Chong PP, Than LT - Front Microbiol (2015)

Bottom Line: Candida glabrata is an emerging human fungal pathogen that has efficacious nutrient sensing and responsiveness ability.It can be seen through its ability to thrive in diverse range of nutrient limited-human anatomical sites.The deletion of SNF3 also resulted in the down-regulation of about half of hexose transporters genes (four out of nine).

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia Serdang, Malaysia.

ABSTRACT
Candida glabrata is an emerging human fungal pathogen that has efficacious nutrient sensing and responsiveness ability. It can be seen through its ability to thrive in diverse range of nutrient limited-human anatomical sites. Therefore, nutrient sensing particularly glucose sensing is thought to be crucial in contributing to the development and fitness of the pathogen. This study aimed to elucidate the role of SNF3 (Sucrose Non Fermenting 3) as a glucose sensor and its possible role in contributing to the fitness and survivability of C. glabrata in glucose-limited environment. The SNF3 knockout strain was constructed and subjected to different glucose concentrations to evaluate its growth, biofilm formation, amphotericin B susceptibility, ex vivo survivability and effects on the transcriptional profiling of the sugar receptor repressor (SRR) pathway-related genes. The CgSNF3Δ strain showed a retarded growth in low glucose environments (0.01 and 0.1%) in both fermentation and respiration-preferred conditions but grew well in high glucose concentration environments (1 and 2%). It was also found to be more susceptible to amphotericin B in low glucose environment (0.1%) and macrophage engulfment but showed no difference in the biofilm formation capability. The deletion of SNF3 also resulted in the down-regulation of about half of hexose transporters genes (four out of nine). Overall, the deletion of SNF3 causes significant reduction in the ability of C. glabrata to sense limited surrounding glucose and consequently disrupts its competency to transport and perform the uptake of this critical nutrient. This study highlighted the role of SNF3 as a high affinity glucose sensor and its role in aiding the survivability of C. glabrata particularly in glucose limited environment.

No MeSH data available.


Related in: MedlinePlus

Biofilm formation activity of Candida glabrata BG2 and SNF3Δ strains under 0.01 and 0.1% glucose concentration. Unpaired T-test was carried out for the statistical analysis to examine the significant difference between BG2 and SNF3Δ and no significant difference was found.
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Figure 4: Biofilm formation activity of Candida glabrata BG2 and SNF3Δ strains under 0.01 and 0.1% glucose concentration. Unpaired T-test was carried out for the statistical analysis to examine the significant difference between BG2 and SNF3Δ and no significant difference was found.

Mentions: Previous study demonstrated the effects of glucose levels in directing C. albicans to form biofilm. Candida albicans tends to form biofilm in low glucose environment and lives in planktonic form in higher glucose environment (Uppuluri et al., 2010; Ng et al., 2015b). The sensitivity of SNF3 in responding to surrounding glucose leads to the thought whether this putative high affinity glucose sensor could contribute in detecting the flow of surrounding glucose and therefore orchestrates the biofilm/planktonic living form of Candida species in accordance to the availability of glucose. Result showed SNF3 did not participate in the biofilm formation of C. glabrata in low glucose environment as no significant differences were found between BG2 and SNF3Δ in the 0.01 and 0.1% glucose tested, respectively (Figure 4).


SNF3 as High Affinity Glucose Sensor and Its Function in Supporting the Viability of Candida glabrata under Glucose-Limited Environment.

Ng TS, Chew SY, Rangasamy P, Mohd Desa MN, Sandai D, Chong PP, Than LT - Front Microbiol (2015)

Biofilm formation activity of Candida glabrata BG2 and SNF3Δ strains under 0.01 and 0.1% glucose concentration. Unpaired T-test was carried out for the statistical analysis to examine the significant difference between BG2 and SNF3Δ and no significant difference was found.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4664639&req=5

Figure 4: Biofilm formation activity of Candida glabrata BG2 and SNF3Δ strains under 0.01 and 0.1% glucose concentration. Unpaired T-test was carried out for the statistical analysis to examine the significant difference between BG2 and SNF3Δ and no significant difference was found.
Mentions: Previous study demonstrated the effects of glucose levels in directing C. albicans to form biofilm. Candida albicans tends to form biofilm in low glucose environment and lives in planktonic form in higher glucose environment (Uppuluri et al., 2010; Ng et al., 2015b). The sensitivity of SNF3 in responding to surrounding glucose leads to the thought whether this putative high affinity glucose sensor could contribute in detecting the flow of surrounding glucose and therefore orchestrates the biofilm/planktonic living form of Candida species in accordance to the availability of glucose. Result showed SNF3 did not participate in the biofilm formation of C. glabrata in low glucose environment as no significant differences were found between BG2 and SNF3Δ in the 0.01 and 0.1% glucose tested, respectively (Figure 4).

Bottom Line: Candida glabrata is an emerging human fungal pathogen that has efficacious nutrient sensing and responsiveness ability.It can be seen through its ability to thrive in diverse range of nutrient limited-human anatomical sites.The deletion of SNF3 also resulted in the down-regulation of about half of hexose transporters genes (four out of nine).

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia Serdang, Malaysia.

ABSTRACT
Candida glabrata is an emerging human fungal pathogen that has efficacious nutrient sensing and responsiveness ability. It can be seen through its ability to thrive in diverse range of nutrient limited-human anatomical sites. Therefore, nutrient sensing particularly glucose sensing is thought to be crucial in contributing to the development and fitness of the pathogen. This study aimed to elucidate the role of SNF3 (Sucrose Non Fermenting 3) as a glucose sensor and its possible role in contributing to the fitness and survivability of C. glabrata in glucose-limited environment. The SNF3 knockout strain was constructed and subjected to different glucose concentrations to evaluate its growth, biofilm formation, amphotericin B susceptibility, ex vivo survivability and effects on the transcriptional profiling of the sugar receptor repressor (SRR) pathway-related genes. The CgSNF3Δ strain showed a retarded growth in low glucose environments (0.01 and 0.1%) in both fermentation and respiration-preferred conditions but grew well in high glucose concentration environments (1 and 2%). It was also found to be more susceptible to amphotericin B in low glucose environment (0.1%) and macrophage engulfment but showed no difference in the biofilm formation capability. The deletion of SNF3 also resulted in the down-regulation of about half of hexose transporters genes (four out of nine). Overall, the deletion of SNF3 causes significant reduction in the ability of C. glabrata to sense limited surrounding glucose and consequently disrupts its competency to transport and perform the uptake of this critical nutrient. This study highlighted the role of SNF3 as a high affinity glucose sensor and its role in aiding the survivability of C. glabrata particularly in glucose limited environment.

No MeSH data available.


Related in: MedlinePlus