Limits...
Involvement of the Cohesin Cofactor PDS5 (SPO76) During Meiosis and DNA Repair in Arabidopsis thaliana.

Pradillo M, Knoll A, Oliver C, Varas J, Corredor E, Puchta H, Santos JL - Front Plant Sci (2015)

Bottom Line: Cohesin cofactors contribute to cohesin dynamics and interact with cohesin complexes during cell cycle.In Arabidopsis, AtWAPL proteins are essential during meiosis, however, the role of AtPDS5 remains to be ascertained.Furthermore, this cohesin cofactor could be important for the function of the AtSMC5/AtSMC6 complex.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Genética, Facultad de Biología, Universidad Complutense Madrid, Spain.

ABSTRACT
Maintenance and precise regulation of sister chromatid cohesion is essential for faithful chromosome segregation during mitosis and meiosis. Cohesin cofactors contribute to cohesin dynamics and interact with cohesin complexes during cell cycle. One of these, PDS5, also known as SPO76, is essential during mitosis and meiosis in several organisms and also plays a role in DNA repair. In yeast, the complex Wapl-Pds5 controls cohesion maintenance and colocalizes with cohesin complexes into chromosomes. In Arabidopsis, AtWAPL proteins are essential during meiosis, however, the role of AtPDS5 remains to be ascertained. Here we have isolated mutants for each of the five AtPDS5 genes (A-E) and obtained, after different crosses between them, double, triple, and even quadruple mutants (Atpds5a Atpds5b Atpds5c Atpds5e). Depletion of AtPDS5 proteins has a weak impact on meiosis, but leads to severe effects on development, fertility, somatic homologous recombination (HR) and DNA repair. Furthermore, this cohesin cofactor could be important for the function of the AtSMC5/AtSMC6 complex. Contrarily to its function in other species, our results suggest that AtPDS5 is dispensable during the meiotic division of Arabidopsis, although it plays an important role in DNA repair by HR.

No MeSH data available.


Related in: MedlinePlus

Meiotic chromosome axes are normal in Atpds5a Atpds5b Atpds5c Atpds5e as revealed by immunolocalization of AtSMC3 and SYN1 at pachytene meiocytes.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4664637&req=5

Figure 3: Meiotic chromosome axes are normal in Atpds5a Atpds5b Atpds5c Atpds5e as revealed by immunolocalization of AtSMC3 and SYN1 at pachytene meiocytes.

Mentions: To further investigate any effect on chromosome axis morphogenesis, chromosome spread preparations of Atpds5a Atpds5b Atpds5c Atpds5e PMCs were examined by using anti-AtSMC3 and anti-SYN1 antibodies in conjunction with an antibody against the synaptonemal transverse filament protein, AtZYP1, to analyze the chronology of the early prophase I stages. AtZYP1 appears at early zygotene as short stretches which extend and produce a continuous signal between the synapsed homologous chromosomes at pachytene. AtSMC3 and SYN1 colocalize with chromosome axes during early prophase I. There were no obvious differences between WT and Atpds5a Atpds5b Atpds5c Atpds5e (n = 20) (Figure 3). This suggests that both proteins, AtSMC3 and SYN1, load normally on mutant chromosomes. Hence, AtPDS5 seems to be dispensable for meiotic cohesin complex formation.


Involvement of the Cohesin Cofactor PDS5 (SPO76) During Meiosis and DNA Repair in Arabidopsis thaliana.

Pradillo M, Knoll A, Oliver C, Varas J, Corredor E, Puchta H, Santos JL - Front Plant Sci (2015)

Meiotic chromosome axes are normal in Atpds5a Atpds5b Atpds5c Atpds5e as revealed by immunolocalization of AtSMC3 and SYN1 at pachytene meiocytes.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4664637&req=5

Figure 3: Meiotic chromosome axes are normal in Atpds5a Atpds5b Atpds5c Atpds5e as revealed by immunolocalization of AtSMC3 and SYN1 at pachytene meiocytes.
Mentions: To further investigate any effect on chromosome axis morphogenesis, chromosome spread preparations of Atpds5a Atpds5b Atpds5c Atpds5e PMCs were examined by using anti-AtSMC3 and anti-SYN1 antibodies in conjunction with an antibody against the synaptonemal transverse filament protein, AtZYP1, to analyze the chronology of the early prophase I stages. AtZYP1 appears at early zygotene as short stretches which extend and produce a continuous signal between the synapsed homologous chromosomes at pachytene. AtSMC3 and SYN1 colocalize with chromosome axes during early prophase I. There were no obvious differences between WT and Atpds5a Atpds5b Atpds5c Atpds5e (n = 20) (Figure 3). This suggests that both proteins, AtSMC3 and SYN1, load normally on mutant chromosomes. Hence, AtPDS5 seems to be dispensable for meiotic cohesin complex formation.

Bottom Line: Cohesin cofactors contribute to cohesin dynamics and interact with cohesin complexes during cell cycle.In Arabidopsis, AtWAPL proteins are essential during meiosis, however, the role of AtPDS5 remains to be ascertained.Furthermore, this cohesin cofactor could be important for the function of the AtSMC5/AtSMC6 complex.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Genética, Facultad de Biología, Universidad Complutense Madrid, Spain.

ABSTRACT
Maintenance and precise regulation of sister chromatid cohesion is essential for faithful chromosome segregation during mitosis and meiosis. Cohesin cofactors contribute to cohesin dynamics and interact with cohesin complexes during cell cycle. One of these, PDS5, also known as SPO76, is essential during mitosis and meiosis in several organisms and also plays a role in DNA repair. In yeast, the complex Wapl-Pds5 controls cohesion maintenance and colocalizes with cohesin complexes into chromosomes. In Arabidopsis, AtWAPL proteins are essential during meiosis, however, the role of AtPDS5 remains to be ascertained. Here we have isolated mutants for each of the five AtPDS5 genes (A-E) and obtained, after different crosses between them, double, triple, and even quadruple mutants (Atpds5a Atpds5b Atpds5c Atpds5e). Depletion of AtPDS5 proteins has a weak impact on meiosis, but leads to severe effects on development, fertility, somatic homologous recombination (HR) and DNA repair. Furthermore, this cohesin cofactor could be important for the function of the AtSMC5/AtSMC6 complex. Contrarily to its function in other species, our results suggest that AtPDS5 is dispensable during the meiotic division of Arabidopsis, although it plays an important role in DNA repair by HR.

No MeSH data available.


Related in: MedlinePlus