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Involvement of the Cohesin Cofactor PDS5 (SPO76) During Meiosis and DNA Repair in Arabidopsis thaliana.

Pradillo M, Knoll A, Oliver C, Varas J, Corredor E, Puchta H, Santos JL - Front Plant Sci (2015)

Bottom Line: Cohesin cofactors contribute to cohesin dynamics and interact with cohesin complexes during cell cycle.In Arabidopsis, AtWAPL proteins are essential during meiosis, however, the role of AtPDS5 remains to be ascertained.Furthermore, this cohesin cofactor could be important for the function of the AtSMC5/AtSMC6 complex.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Genética, Facultad de Biología, Universidad Complutense Madrid, Spain.

ABSTRACT
Maintenance and precise regulation of sister chromatid cohesion is essential for faithful chromosome segregation during mitosis and meiosis. Cohesin cofactors contribute to cohesin dynamics and interact with cohesin complexes during cell cycle. One of these, PDS5, also known as SPO76, is essential during mitosis and meiosis in several organisms and also plays a role in DNA repair. In yeast, the complex Wapl-Pds5 controls cohesion maintenance and colocalizes with cohesin complexes into chromosomes. In Arabidopsis, AtWAPL proteins are essential during meiosis, however, the role of AtPDS5 remains to be ascertained. Here we have isolated mutants for each of the five AtPDS5 genes (A-E) and obtained, after different crosses between them, double, triple, and even quadruple mutants (Atpds5a Atpds5b Atpds5c Atpds5e). Depletion of AtPDS5 proteins has a weak impact on meiosis, but leads to severe effects on development, fertility, somatic homologous recombination (HR) and DNA repair. Furthermore, this cohesin cofactor could be important for the function of the AtSMC5/AtSMC6 complex. Contrarily to its function in other species, our results suggest that AtPDS5 is dispensable during the meiotic division of Arabidopsis, although it plays an important role in DNA repair by HR.

No MeSH data available.


Related in: MedlinePlus

Loss of AtPDS5 function generates chromosome bridges at anaphase I and telophase I. Arrows indicate chromosome bridges, which probably arise because of the existence of unresolved recombination intermediates, and a fragment.
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Figure 2: Loss of AtPDS5 function generates chromosome bridges at anaphase I and telophase I. Arrows indicate chromosome bridges, which probably arise because of the existence of unresolved recombination intermediates, and a fragment.

Mentions: To determine whether meiosis defects could be responsible for the reduced fertility of Atpds5 mutants, we analyzed DAPI-stained chromosome spreads from PMCs. Analysis of PMCs in Atpds5 mutants revealed that meiosis proceeds without any important deviation from WT, even in the quadruple mutant (Supplementary Figures S3 and S4). We only detected, in contrast to the WT, the presence of some chromosome bridges at late anaphase I and telophase I in the single mutants, ranging from 20 to 25% (n = 40) (Figure 2). These bridges, originated probably as consequence of unresolved recombination intermediates, were also observed in the quadruple mutant with a similar frequency (22.5%; n = 40) and gave rise to fragments with a very low frequency (Figure 2).


Involvement of the Cohesin Cofactor PDS5 (SPO76) During Meiosis and DNA Repair in Arabidopsis thaliana.

Pradillo M, Knoll A, Oliver C, Varas J, Corredor E, Puchta H, Santos JL - Front Plant Sci (2015)

Loss of AtPDS5 function generates chromosome bridges at anaphase I and telophase I. Arrows indicate chromosome bridges, which probably arise because of the existence of unresolved recombination intermediates, and a fragment.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4664637&req=5

Figure 2: Loss of AtPDS5 function generates chromosome bridges at anaphase I and telophase I. Arrows indicate chromosome bridges, which probably arise because of the existence of unresolved recombination intermediates, and a fragment.
Mentions: To determine whether meiosis defects could be responsible for the reduced fertility of Atpds5 mutants, we analyzed DAPI-stained chromosome spreads from PMCs. Analysis of PMCs in Atpds5 mutants revealed that meiosis proceeds without any important deviation from WT, even in the quadruple mutant (Supplementary Figures S3 and S4). We only detected, in contrast to the WT, the presence of some chromosome bridges at late anaphase I and telophase I in the single mutants, ranging from 20 to 25% (n = 40) (Figure 2). These bridges, originated probably as consequence of unresolved recombination intermediates, were also observed in the quadruple mutant with a similar frequency (22.5%; n = 40) and gave rise to fragments with a very low frequency (Figure 2).

Bottom Line: Cohesin cofactors contribute to cohesin dynamics and interact with cohesin complexes during cell cycle.In Arabidopsis, AtWAPL proteins are essential during meiosis, however, the role of AtPDS5 remains to be ascertained.Furthermore, this cohesin cofactor could be important for the function of the AtSMC5/AtSMC6 complex.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Genética, Facultad de Biología, Universidad Complutense Madrid, Spain.

ABSTRACT
Maintenance and precise regulation of sister chromatid cohesion is essential for faithful chromosome segregation during mitosis and meiosis. Cohesin cofactors contribute to cohesin dynamics and interact with cohesin complexes during cell cycle. One of these, PDS5, also known as SPO76, is essential during mitosis and meiosis in several organisms and also plays a role in DNA repair. In yeast, the complex Wapl-Pds5 controls cohesion maintenance and colocalizes with cohesin complexes into chromosomes. In Arabidopsis, AtWAPL proteins are essential during meiosis, however, the role of AtPDS5 remains to be ascertained. Here we have isolated mutants for each of the five AtPDS5 genes (A-E) and obtained, after different crosses between them, double, triple, and even quadruple mutants (Atpds5a Atpds5b Atpds5c Atpds5e). Depletion of AtPDS5 proteins has a weak impact on meiosis, but leads to severe effects on development, fertility, somatic homologous recombination (HR) and DNA repair. Furthermore, this cohesin cofactor could be important for the function of the AtSMC5/AtSMC6 complex. Contrarily to its function in other species, our results suggest that AtPDS5 is dispensable during the meiotic division of Arabidopsis, although it plays an important role in DNA repair by HR.

No MeSH data available.


Related in: MedlinePlus