Limits...
Involvement of the Cohesin Cofactor PDS5 (SPO76) During Meiosis and DNA Repair in Arabidopsis thaliana.

Pradillo M, Knoll A, Oliver C, Varas J, Corredor E, Puchta H, Santos JL - Front Plant Sci (2015)

Bottom Line: Cohesin cofactors contribute to cohesin dynamics and interact with cohesin complexes during cell cycle.In Arabidopsis, AtWAPL proteins are essential during meiosis, however, the role of AtPDS5 remains to be ascertained.Furthermore, this cohesin cofactor could be important for the function of the AtSMC5/AtSMC6 complex.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Genética, Facultad de Biología, Universidad Complutense Madrid, Spain.

ABSTRACT
Maintenance and precise regulation of sister chromatid cohesion is essential for faithful chromosome segregation during mitosis and meiosis. Cohesin cofactors contribute to cohesin dynamics and interact with cohesin complexes during cell cycle. One of these, PDS5, also known as SPO76, is essential during mitosis and meiosis in several organisms and also plays a role in DNA repair. In yeast, the complex Wapl-Pds5 controls cohesion maintenance and colocalizes with cohesin complexes into chromosomes. In Arabidopsis, AtWAPL proteins are essential during meiosis, however, the role of AtPDS5 remains to be ascertained. Here we have isolated mutants for each of the five AtPDS5 genes (A-E) and obtained, after different crosses between them, double, triple, and even quadruple mutants (Atpds5a Atpds5b Atpds5c Atpds5e). Depletion of AtPDS5 proteins has a weak impact on meiosis, but leads to severe effects on development, fertility, somatic homologous recombination (HR) and DNA repair. Furthermore, this cohesin cofactor could be important for the function of the AtSMC5/AtSMC6 complex. Contrarily to its function in other species, our results suggest that AtPDS5 is dispensable during the meiotic division of Arabidopsis, although it plays an important role in DNA repair by HR.

No MeSH data available.


Related in: MedlinePlus

AtPDS5 genes, AtPDS5 proteins and plant phenotypes of Atpds5 mutants. (A) An illustration of the exon/intron organization of AtPDS5 genes. Exons are shown as black boxes and introns are shown as black lines. UTR regions are shown as gray boxes. Positions of T-DNA insertion sites of the different mutants are marked. (B) Schematic representation of the domains present in AtPDS5 proteins. (C) Chromosomal localization of the five AtPDS5 orthologs. (D) Leaf rosette structure of 1 month-old plants imaged just before (WT, double mutant) or after (triple and quadruple mutants) bolting. (E) Six week-old WT, triple and quadruple mutant plants. (F) Siliques of WT, triple and quadruple mutant plants. ab: Atpds5a Atpds5b; abc: Atpds5a Atpds5b Atpds5c; abce: Atpds5a Atpds5b Atpds5c Atpds5e.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4664637&req=5

Figure 1: AtPDS5 genes, AtPDS5 proteins and plant phenotypes of Atpds5 mutants. (A) An illustration of the exon/intron organization of AtPDS5 genes. Exons are shown as black boxes and introns are shown as black lines. UTR regions are shown as gray boxes. Positions of T-DNA insertion sites of the different mutants are marked. (B) Schematic representation of the domains present in AtPDS5 proteins. (C) Chromosomal localization of the five AtPDS5 orthologs. (D) Leaf rosette structure of 1 month-old plants imaged just before (WT, double mutant) or after (triple and quadruple mutants) bolting. (E) Six week-old WT, triple and quadruple mutant plants. (F) Siliques of WT, triple and quadruple mutant plants. ab: Atpds5a Atpds5b; abc: Atpds5a Atpds5b Atpds5c; abce: Atpds5a Atpds5b Atpds5c Atpds5e.

Mentions: According to previous results and database searches of putative PDS5 genes in the Arabidopsis genome, five candidates were identified (Mercier et al., 2001; Figures 1A,C). The protein that displays the highest identity and similarity to S. macrospora sequences (SPO76, 15% identity and 27% similarity), A. nidulans (BIMD, 15% identity and 26% similarity) and Mus musculus (PDS5A, 22% identity and 30% similarity; and PDS5B, 22% identity and 32% similarity) is encoded by At5g47690. For this reason we designated it as AtPDS5A. The remaining proteins were named according to their identity (ranging from 31 to 23%) and similarity (ranging from 39 to 20%) respect to AtPDS5A: AtPDS5B (At1g77600), AtPDS5C (At4g31880), AtPDS5D (At1g80810) and AtPDS5E (At1g15940). AtPDS5D and AtPDS5E are the most similar to each other (53%). As well as PDS5 proteins from other organisms, according to UniProt database, all AtPDS5 proteins contain an Armadillo-type fold domain in the or near to the N-terminus (UniProt Consortium, 2015; Figure 1B). This domain presents an extensive solvent-accessible surface that promotes interactions with proteins and nucleic acids.


Involvement of the Cohesin Cofactor PDS5 (SPO76) During Meiosis and DNA Repair in Arabidopsis thaliana.

Pradillo M, Knoll A, Oliver C, Varas J, Corredor E, Puchta H, Santos JL - Front Plant Sci (2015)

AtPDS5 genes, AtPDS5 proteins and plant phenotypes of Atpds5 mutants. (A) An illustration of the exon/intron organization of AtPDS5 genes. Exons are shown as black boxes and introns are shown as black lines. UTR regions are shown as gray boxes. Positions of T-DNA insertion sites of the different mutants are marked. (B) Schematic representation of the domains present in AtPDS5 proteins. (C) Chromosomal localization of the five AtPDS5 orthologs. (D) Leaf rosette structure of 1 month-old plants imaged just before (WT, double mutant) or after (triple and quadruple mutants) bolting. (E) Six week-old WT, triple and quadruple mutant plants. (F) Siliques of WT, triple and quadruple mutant plants. ab: Atpds5a Atpds5b; abc: Atpds5a Atpds5b Atpds5c; abce: Atpds5a Atpds5b Atpds5c Atpds5e.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4664637&req=5

Figure 1: AtPDS5 genes, AtPDS5 proteins and plant phenotypes of Atpds5 mutants. (A) An illustration of the exon/intron organization of AtPDS5 genes. Exons are shown as black boxes and introns are shown as black lines. UTR regions are shown as gray boxes. Positions of T-DNA insertion sites of the different mutants are marked. (B) Schematic representation of the domains present in AtPDS5 proteins. (C) Chromosomal localization of the five AtPDS5 orthologs. (D) Leaf rosette structure of 1 month-old plants imaged just before (WT, double mutant) or after (triple and quadruple mutants) bolting. (E) Six week-old WT, triple and quadruple mutant plants. (F) Siliques of WT, triple and quadruple mutant plants. ab: Atpds5a Atpds5b; abc: Atpds5a Atpds5b Atpds5c; abce: Atpds5a Atpds5b Atpds5c Atpds5e.
Mentions: According to previous results and database searches of putative PDS5 genes in the Arabidopsis genome, five candidates were identified (Mercier et al., 2001; Figures 1A,C). The protein that displays the highest identity and similarity to S. macrospora sequences (SPO76, 15% identity and 27% similarity), A. nidulans (BIMD, 15% identity and 26% similarity) and Mus musculus (PDS5A, 22% identity and 30% similarity; and PDS5B, 22% identity and 32% similarity) is encoded by At5g47690. For this reason we designated it as AtPDS5A. The remaining proteins were named according to their identity (ranging from 31 to 23%) and similarity (ranging from 39 to 20%) respect to AtPDS5A: AtPDS5B (At1g77600), AtPDS5C (At4g31880), AtPDS5D (At1g80810) and AtPDS5E (At1g15940). AtPDS5D and AtPDS5E are the most similar to each other (53%). As well as PDS5 proteins from other organisms, according to UniProt database, all AtPDS5 proteins contain an Armadillo-type fold domain in the or near to the N-terminus (UniProt Consortium, 2015; Figure 1B). This domain presents an extensive solvent-accessible surface that promotes interactions with proteins and nucleic acids.

Bottom Line: Cohesin cofactors contribute to cohesin dynamics and interact with cohesin complexes during cell cycle.In Arabidopsis, AtWAPL proteins are essential during meiosis, however, the role of AtPDS5 remains to be ascertained.Furthermore, this cohesin cofactor could be important for the function of the AtSMC5/AtSMC6 complex.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Genética, Facultad de Biología, Universidad Complutense Madrid, Spain.

ABSTRACT
Maintenance and precise regulation of sister chromatid cohesion is essential for faithful chromosome segregation during mitosis and meiosis. Cohesin cofactors contribute to cohesin dynamics and interact with cohesin complexes during cell cycle. One of these, PDS5, also known as SPO76, is essential during mitosis and meiosis in several organisms and also plays a role in DNA repair. In yeast, the complex Wapl-Pds5 controls cohesion maintenance and colocalizes with cohesin complexes into chromosomes. In Arabidopsis, AtWAPL proteins are essential during meiosis, however, the role of AtPDS5 remains to be ascertained. Here we have isolated mutants for each of the five AtPDS5 genes (A-E) and obtained, after different crosses between them, double, triple, and even quadruple mutants (Atpds5a Atpds5b Atpds5c Atpds5e). Depletion of AtPDS5 proteins has a weak impact on meiosis, but leads to severe effects on development, fertility, somatic homologous recombination (HR) and DNA repair. Furthermore, this cohesin cofactor could be important for the function of the AtSMC5/AtSMC6 complex. Contrarily to its function in other species, our results suggest that AtPDS5 is dispensable during the meiotic division of Arabidopsis, although it plays an important role in DNA repair by HR.

No MeSH data available.


Related in: MedlinePlus