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Zinc-Dependent Protection of Tobacco and Rice Cells From Aluminum-Induced Superoxide-Mediated Cytotoxicity.

Lin C, Hara A, Comparini D, Bouteau F, Kawano T - Front Plant Sci (2015)

Bottom Line: Earlier studies suggested that lack of zinc often results in ROS-mediated oxidative damage to plant cells.In the present study, the effect of Zn(2+) on Al(3+)-induced superoxide generation in the cell suspension cultures of tobacco (Nicotiana tabacum L., cell-line, BY-2) and rice (Oryza sativa L., cv.Obtained results indicated that presence of Zn(2+) at physiological concentrations can protect the cells by preventing the Al(3+)-induced superoxide generation and cell death.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Environmental Engineering and Graduate School of Environmental Engineering, The University of Kitakyushu , Kitakyushu, Japan.

ABSTRACT
Al(3+) toxicity in growing plants is considered as one of the major factors limiting the production of crops on acidic soils worldwide. In the last 15 years, it has been proposed that Al(3+) toxicity are mediated with distortion of the cellular signaling mechanisms such as calcium signaling pathways, and production of cytotoxic reactive oxygen species (ROS) causing oxidative damages. On the other hand, zinc is normally present in plants at high concentrations and its deficiency is one of the most widespread micronutrient deficiencies in plants. Earlier studies suggested that lack of zinc often results in ROS-mediated oxidative damage to plant cells. Previously, inhibitory action of Zn(2+) against lanthanide-induced superoxide generation in tobacco cells have been reported, suggesting that Zn(2+) interferes with the cation-induced ROS production via stimulation of NADPH oxidase. In the present study, the effect of Zn(2+) on Al(3+)-induced superoxide generation in the cell suspension cultures of tobacco (Nicotiana tabacum L., cell-line, BY-2) and rice (Oryza sativa L., cv. Nipponbare), was examined. The Zn(2+)-dependent inhibition of the Al(3+)-induced oxidative burst was observed in both model cells selected from the monocots and dicots (rice and tobacco), suggesting that this phenomenon (Al(3+)/Zn(2+) interaction) can be preserved in higher plants. Subsequently induced cell death in tobacco cells was analyzed by lethal cell staining with Evans blue. Obtained results indicated that presence of Zn(2+) at physiological concentrations can protect the cells by preventing the Al(3+)-induced superoxide generation and cell death. Furthermore, the regulation of the Ca(2+) signaling, i.e., change in the cytosolic Ca(2+) ion concentration, and the cross-talks among the elements which participate in the pathway were further explored.

No MeSH data available.


Related in: MedlinePlus

Monitoring of Al3+-responsive [Ca2+]c with aequorin luminescence in the presence and absence of various inhibitors. (A) Effect of high- and low-dose Zinc, DPI (NADPH oxidase inhibitor), ROS scavengers (Tiron, DMTU), and trivalent cations (La3+ and Gd3+) on Al-responsive [Ca2+]c elevation in tobacco cell suspension culture expressing aequorin. (Inset) Quantitative comparison of the action of inhibitors against the peak height of Al-responsive [Ca2+]c elevation (n = 3; error bars, SD). (B) Effect of Al3+ concentration on induction of [Ca2+]c elevation. Typical records of Ca2+-responsive aequorin luminescence measured in the presence of different Aluminum concentrations are shown.
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Figure 7: Monitoring of Al3+-responsive [Ca2+]c with aequorin luminescence in the presence and absence of various inhibitors. (A) Effect of high- and low-dose Zinc, DPI (NADPH oxidase inhibitor), ROS scavengers (Tiron, DMTU), and trivalent cations (La3+ and Gd3+) on Al-responsive [Ca2+]c elevation in tobacco cell suspension culture expressing aequorin. (Inset) Quantitative comparison of the action of inhibitors against the peak height of Al-responsive [Ca2+]c elevation (n = 3; error bars, SD). (B) Effect of Al3+ concentration on induction of [Ca2+]c elevation. Typical records of Ca2+-responsive aequorin luminescence measured in the presence of different Aluminum concentrations are shown.

Mentions: To support the hypothesis that Al3+-induced distortion in [Ca2+]c involves the members of ROS derived from the action of NADPH oxidase, and calcium channel opening leading to transient [Ca2+]c elevation, the effect of DPI (NADPH oxidase inhibitor), ROS scavengers (Tiron, DMTU), and trivalent cations (La3+ and Gd3+) have been tested in tobacco cells expressing aequorin and compared with the antagonistic action of zinc protecting the cells (Figure 7A and inset).


Zinc-Dependent Protection of Tobacco and Rice Cells From Aluminum-Induced Superoxide-Mediated Cytotoxicity.

Lin C, Hara A, Comparini D, Bouteau F, Kawano T - Front Plant Sci (2015)

Monitoring of Al3+-responsive [Ca2+]c with aequorin luminescence in the presence and absence of various inhibitors. (A) Effect of high- and low-dose Zinc, DPI (NADPH oxidase inhibitor), ROS scavengers (Tiron, DMTU), and trivalent cations (La3+ and Gd3+) on Al-responsive [Ca2+]c elevation in tobacco cell suspension culture expressing aequorin. (Inset) Quantitative comparison of the action of inhibitors against the peak height of Al-responsive [Ca2+]c elevation (n = 3; error bars, SD). (B) Effect of Al3+ concentration on induction of [Ca2+]c elevation. Typical records of Ca2+-responsive aequorin luminescence measured in the presence of different Aluminum concentrations are shown.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4664629&req=5

Figure 7: Monitoring of Al3+-responsive [Ca2+]c with aequorin luminescence in the presence and absence of various inhibitors. (A) Effect of high- and low-dose Zinc, DPI (NADPH oxidase inhibitor), ROS scavengers (Tiron, DMTU), and trivalent cations (La3+ and Gd3+) on Al-responsive [Ca2+]c elevation in tobacco cell suspension culture expressing aequorin. (Inset) Quantitative comparison of the action of inhibitors against the peak height of Al-responsive [Ca2+]c elevation (n = 3; error bars, SD). (B) Effect of Al3+ concentration on induction of [Ca2+]c elevation. Typical records of Ca2+-responsive aequorin luminescence measured in the presence of different Aluminum concentrations are shown.
Mentions: To support the hypothesis that Al3+-induced distortion in [Ca2+]c involves the members of ROS derived from the action of NADPH oxidase, and calcium channel opening leading to transient [Ca2+]c elevation, the effect of DPI (NADPH oxidase inhibitor), ROS scavengers (Tiron, DMTU), and trivalent cations (La3+ and Gd3+) have been tested in tobacco cells expressing aequorin and compared with the antagonistic action of zinc protecting the cells (Figure 7A and inset).

Bottom Line: Earlier studies suggested that lack of zinc often results in ROS-mediated oxidative damage to plant cells.In the present study, the effect of Zn(2+) on Al(3+)-induced superoxide generation in the cell suspension cultures of tobacco (Nicotiana tabacum L., cell-line, BY-2) and rice (Oryza sativa L., cv.Obtained results indicated that presence of Zn(2+) at physiological concentrations can protect the cells by preventing the Al(3+)-induced superoxide generation and cell death.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Environmental Engineering and Graduate School of Environmental Engineering, The University of Kitakyushu , Kitakyushu, Japan.

ABSTRACT
Al(3+) toxicity in growing plants is considered as one of the major factors limiting the production of crops on acidic soils worldwide. In the last 15 years, it has been proposed that Al(3+) toxicity are mediated with distortion of the cellular signaling mechanisms such as calcium signaling pathways, and production of cytotoxic reactive oxygen species (ROS) causing oxidative damages. On the other hand, zinc is normally present in plants at high concentrations and its deficiency is one of the most widespread micronutrient deficiencies in plants. Earlier studies suggested that lack of zinc often results in ROS-mediated oxidative damage to plant cells. Previously, inhibitory action of Zn(2+) against lanthanide-induced superoxide generation in tobacco cells have been reported, suggesting that Zn(2+) interferes with the cation-induced ROS production via stimulation of NADPH oxidase. In the present study, the effect of Zn(2+) on Al(3+)-induced superoxide generation in the cell suspension cultures of tobacco (Nicotiana tabacum L., cell-line, BY-2) and rice (Oryza sativa L., cv. Nipponbare), was examined. The Zn(2+)-dependent inhibition of the Al(3+)-induced oxidative burst was observed in both model cells selected from the monocots and dicots (rice and tobacco), suggesting that this phenomenon (Al(3+)/Zn(2+) interaction) can be preserved in higher plants. Subsequently induced cell death in tobacco cells was analyzed by lethal cell staining with Evans blue. Obtained results indicated that presence of Zn(2+) at physiological concentrations can protect the cells by preventing the Al(3+)-induced superoxide generation and cell death. Furthermore, the regulation of the Ca(2+) signaling, i.e., change in the cytosolic Ca(2+) ion concentration, and the cross-talks among the elements which participate in the pathway were further explored.

No MeSH data available.


Related in: MedlinePlus