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Zinc-Dependent Protection of Tobacco and Rice Cells From Aluminum-Induced Superoxide-Mediated Cytotoxicity.

Lin C, Hara A, Comparini D, Bouteau F, Kawano T - Front Plant Sci (2015)

Bottom Line: Earlier studies suggested that lack of zinc often results in ROS-mediated oxidative damage to plant cells.In the present study, the effect of Zn(2+) on Al(3+)-induced superoxide generation in the cell suspension cultures of tobacco (Nicotiana tabacum L., cell-line, BY-2) and rice (Oryza sativa L., cv.Obtained results indicated that presence of Zn(2+) at physiological concentrations can protect the cells by preventing the Al(3+)-induced superoxide generation and cell death.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Environmental Engineering and Graduate School of Environmental Engineering, The University of Kitakyushu , Kitakyushu, Japan.

ABSTRACT
Al(3+) toxicity in growing plants is considered as one of the major factors limiting the production of crops on acidic soils worldwide. In the last 15 years, it has been proposed that Al(3+) toxicity are mediated with distortion of the cellular signaling mechanisms such as calcium signaling pathways, and production of cytotoxic reactive oxygen species (ROS) causing oxidative damages. On the other hand, zinc is normally present in plants at high concentrations and its deficiency is one of the most widespread micronutrient deficiencies in plants. Earlier studies suggested that lack of zinc often results in ROS-mediated oxidative damage to plant cells. Previously, inhibitory action of Zn(2+) against lanthanide-induced superoxide generation in tobacco cells have been reported, suggesting that Zn(2+) interferes with the cation-induced ROS production via stimulation of NADPH oxidase. In the present study, the effect of Zn(2+) on Al(3+)-induced superoxide generation in the cell suspension cultures of tobacco (Nicotiana tabacum L., cell-line, BY-2) and rice (Oryza sativa L., cv. Nipponbare), was examined. The Zn(2+)-dependent inhibition of the Al(3+)-induced oxidative burst was observed in both model cells selected from the monocots and dicots (rice and tobacco), suggesting that this phenomenon (Al(3+)/Zn(2+) interaction) can be preserved in higher plants. Subsequently induced cell death in tobacco cells was analyzed by lethal cell staining with Evans blue. Obtained results indicated that presence of Zn(2+) at physiological concentrations can protect the cells by preventing the Al(3+)-induced superoxide generation and cell death. Furthermore, the regulation of the Ca(2+) signaling, i.e., change in the cytosolic Ca(2+) ion concentration, and the cross-talks among the elements which participate in the pathway were further explored.

No MeSH data available.


Related in: MedlinePlus

Effect of culture age on Al3+-induced O2•– generation in tobacco cell suspension culture. (A) Growth curve for tobacco cells. (B) Four different patterns of CLA-CL reflecting the Al3+-induced O2•– generation.
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Figure 4: Effect of culture age on Al3+-induced O2•– generation in tobacco cell suspension culture. (A) Growth curve for tobacco cells. (B) Four different patterns of CLA-CL reflecting the Al3+-induced O2•– generation.

Mentions: Prior to treatment with Al3+, tobacco BY-2 cell suspension culture was aged for 1, 3, 5, 8, and 13 DAI of the fresh media (30 ml) with 0.5 ml of confluent culture (at 10 DAI). The cultures at 1 and 3 DAI were smooth and colorless. The 4 and 5 DAI cultures were also smooth but colored slightly yellowish. The 8 and 12 DAI cultures were highly dense and colored yellow. The growth of the culture was assessed by measuring the changes in fresh cell weight at each time point. Figure 4A shows a typical growth curve for tobacco BY-2 cell culture. Effect of culture age of tobacco BY-2 cells on the sensitivity to Al3+ was examined using the differently aged cultures (Figure 4B), and the high sensitivity to Al3+ was observed in 2 and 4 DAI of tobacco BY-2 cells.


Zinc-Dependent Protection of Tobacco and Rice Cells From Aluminum-Induced Superoxide-Mediated Cytotoxicity.

Lin C, Hara A, Comparini D, Bouteau F, Kawano T - Front Plant Sci (2015)

Effect of culture age on Al3+-induced O2•– generation in tobacco cell suspension culture. (A) Growth curve for tobacco cells. (B) Four different patterns of CLA-CL reflecting the Al3+-induced O2•– generation.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4664629&req=5

Figure 4: Effect of culture age on Al3+-induced O2•– generation in tobacco cell suspension culture. (A) Growth curve for tobacco cells. (B) Four different patterns of CLA-CL reflecting the Al3+-induced O2•– generation.
Mentions: Prior to treatment with Al3+, tobacco BY-2 cell suspension culture was aged for 1, 3, 5, 8, and 13 DAI of the fresh media (30 ml) with 0.5 ml of confluent culture (at 10 DAI). The cultures at 1 and 3 DAI were smooth and colorless. The 4 and 5 DAI cultures were also smooth but colored slightly yellowish. The 8 and 12 DAI cultures were highly dense and colored yellow. The growth of the culture was assessed by measuring the changes in fresh cell weight at each time point. Figure 4A shows a typical growth curve for tobacco BY-2 cell culture. Effect of culture age of tobacco BY-2 cells on the sensitivity to Al3+ was examined using the differently aged cultures (Figure 4B), and the high sensitivity to Al3+ was observed in 2 and 4 DAI of tobacco BY-2 cells.

Bottom Line: Earlier studies suggested that lack of zinc often results in ROS-mediated oxidative damage to plant cells.In the present study, the effect of Zn(2+) on Al(3+)-induced superoxide generation in the cell suspension cultures of tobacco (Nicotiana tabacum L., cell-line, BY-2) and rice (Oryza sativa L., cv.Obtained results indicated that presence of Zn(2+) at physiological concentrations can protect the cells by preventing the Al(3+)-induced superoxide generation and cell death.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Environmental Engineering and Graduate School of Environmental Engineering, The University of Kitakyushu , Kitakyushu, Japan.

ABSTRACT
Al(3+) toxicity in growing plants is considered as one of the major factors limiting the production of crops on acidic soils worldwide. In the last 15 years, it has been proposed that Al(3+) toxicity are mediated with distortion of the cellular signaling mechanisms such as calcium signaling pathways, and production of cytotoxic reactive oxygen species (ROS) causing oxidative damages. On the other hand, zinc is normally present in plants at high concentrations and its deficiency is one of the most widespread micronutrient deficiencies in plants. Earlier studies suggested that lack of zinc often results in ROS-mediated oxidative damage to plant cells. Previously, inhibitory action of Zn(2+) against lanthanide-induced superoxide generation in tobacco cells have been reported, suggesting that Zn(2+) interferes with the cation-induced ROS production via stimulation of NADPH oxidase. In the present study, the effect of Zn(2+) on Al(3+)-induced superoxide generation in the cell suspension cultures of tobacco (Nicotiana tabacum L., cell-line, BY-2) and rice (Oryza sativa L., cv. Nipponbare), was examined. The Zn(2+)-dependent inhibition of the Al(3+)-induced oxidative burst was observed in both model cells selected from the monocots and dicots (rice and tobacco), suggesting that this phenomenon (Al(3+)/Zn(2+) interaction) can be preserved in higher plants. Subsequently induced cell death in tobacco cells was analyzed by lethal cell staining with Evans blue. Obtained results indicated that presence of Zn(2+) at physiological concentrations can protect the cells by preventing the Al(3+)-induced superoxide generation and cell death. Furthermore, the regulation of the Ca(2+) signaling, i.e., change in the cytosolic Ca(2+) ion concentration, and the cross-talks among the elements which participate in the pathway were further explored.

No MeSH data available.


Related in: MedlinePlus