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Zinc-Dependent Protection of Tobacco and Rice Cells From Aluminum-Induced Superoxide-Mediated Cytotoxicity.

Lin C, Hara A, Comparini D, Bouteau F, Kawano T - Front Plant Sci (2015)

Bottom Line: Earlier studies suggested that lack of zinc often results in ROS-mediated oxidative damage to plant cells.In the present study, the effect of Zn(2+) on Al(3+)-induced superoxide generation in the cell suspension cultures of tobacco (Nicotiana tabacum L., cell-line, BY-2) and rice (Oryza sativa L., cv.Obtained results indicated that presence of Zn(2+) at physiological concentrations can protect the cells by preventing the Al(3+)-induced superoxide generation and cell death.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Environmental Engineering and Graduate School of Environmental Engineering, The University of Kitakyushu , Kitakyushu, Japan.

ABSTRACT
Al(3+) toxicity in growing plants is considered as one of the major factors limiting the production of crops on acidic soils worldwide. In the last 15 years, it has been proposed that Al(3+) toxicity are mediated with distortion of the cellular signaling mechanisms such as calcium signaling pathways, and production of cytotoxic reactive oxygen species (ROS) causing oxidative damages. On the other hand, zinc is normally present in plants at high concentrations and its deficiency is one of the most widespread micronutrient deficiencies in plants. Earlier studies suggested that lack of zinc often results in ROS-mediated oxidative damage to plant cells. Previously, inhibitory action of Zn(2+) against lanthanide-induced superoxide generation in tobacco cells have been reported, suggesting that Zn(2+) interferes with the cation-induced ROS production via stimulation of NADPH oxidase. In the present study, the effect of Zn(2+) on Al(3+)-induced superoxide generation in the cell suspension cultures of tobacco (Nicotiana tabacum L., cell-line, BY-2) and rice (Oryza sativa L., cv. Nipponbare), was examined. The Zn(2+)-dependent inhibition of the Al(3+)-induced oxidative burst was observed in both model cells selected from the monocots and dicots (rice and tobacco), suggesting that this phenomenon (Al(3+)/Zn(2+) interaction) can be preserved in higher plants. Subsequently induced cell death in tobacco cells was analyzed by lethal cell staining with Evans blue. Obtained results indicated that presence of Zn(2+) at physiological concentrations can protect the cells by preventing the Al(3+)-induced superoxide generation and cell death. Furthermore, the regulation of the Ca(2+) signaling, i.e., change in the cytosolic Ca(2+) ion concentration, and the cross-talks among the elements which participate in the pathway were further explored.

No MeSH data available.


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Microscopic images of plant cells used in this study. (A) Tobacco BY-2 cells. (B) Rice M1 cells. Scale bars, 50 μm.
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Figure 1: Microscopic images of plant cells used in this study. (A) Tobacco BY-2 cells. (B) Rice M1 cells. Scale bars, 50 μm.

Mentions: Tobacco (Nicotiana tabacum L. cv. Bright Yellow-2) suspension-culture cells (cell line, BY-2, expressing the aequorin gene; Figure 1A) were propagated as previously described (Kawano et al., 1998). Briefly, the culture was maintained in Murashige–Skoog liquid medium (pH 5.8) supplemented with 3% (w/v) sucrose and 0.2 μg ml–1 of 2,4-dichlorophenoxyacetic acid. The culture was propagated with shaking on a gyratory shaker in darkness at 23°C. For sub-culturing, 1.0 ml of confluent stationary culture was suspended in 30 ml of fresh culture medium and incubated at 23°C with shaking at 130 rpm on a gyratory shaker in darkness until used.


Zinc-Dependent Protection of Tobacco and Rice Cells From Aluminum-Induced Superoxide-Mediated Cytotoxicity.

Lin C, Hara A, Comparini D, Bouteau F, Kawano T - Front Plant Sci (2015)

Microscopic images of plant cells used in this study. (A) Tobacco BY-2 cells. (B) Rice M1 cells. Scale bars, 50 μm.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4664629&req=5

Figure 1: Microscopic images of plant cells used in this study. (A) Tobacco BY-2 cells. (B) Rice M1 cells. Scale bars, 50 μm.
Mentions: Tobacco (Nicotiana tabacum L. cv. Bright Yellow-2) suspension-culture cells (cell line, BY-2, expressing the aequorin gene; Figure 1A) were propagated as previously described (Kawano et al., 1998). Briefly, the culture was maintained in Murashige–Skoog liquid medium (pH 5.8) supplemented with 3% (w/v) sucrose and 0.2 μg ml–1 of 2,4-dichlorophenoxyacetic acid. The culture was propagated with shaking on a gyratory shaker in darkness at 23°C. For sub-culturing, 1.0 ml of confluent stationary culture was suspended in 30 ml of fresh culture medium and incubated at 23°C with shaking at 130 rpm on a gyratory shaker in darkness until used.

Bottom Line: Earlier studies suggested that lack of zinc often results in ROS-mediated oxidative damage to plant cells.In the present study, the effect of Zn(2+) on Al(3+)-induced superoxide generation in the cell suspension cultures of tobacco (Nicotiana tabacum L., cell-line, BY-2) and rice (Oryza sativa L., cv.Obtained results indicated that presence of Zn(2+) at physiological concentrations can protect the cells by preventing the Al(3+)-induced superoxide generation and cell death.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Environmental Engineering and Graduate School of Environmental Engineering, The University of Kitakyushu , Kitakyushu, Japan.

ABSTRACT
Al(3+) toxicity in growing plants is considered as one of the major factors limiting the production of crops on acidic soils worldwide. In the last 15 years, it has been proposed that Al(3+) toxicity are mediated with distortion of the cellular signaling mechanisms such as calcium signaling pathways, and production of cytotoxic reactive oxygen species (ROS) causing oxidative damages. On the other hand, zinc is normally present in plants at high concentrations and its deficiency is one of the most widespread micronutrient deficiencies in plants. Earlier studies suggested that lack of zinc often results in ROS-mediated oxidative damage to plant cells. Previously, inhibitory action of Zn(2+) against lanthanide-induced superoxide generation in tobacco cells have been reported, suggesting that Zn(2+) interferes with the cation-induced ROS production via stimulation of NADPH oxidase. In the present study, the effect of Zn(2+) on Al(3+)-induced superoxide generation in the cell suspension cultures of tobacco (Nicotiana tabacum L., cell-line, BY-2) and rice (Oryza sativa L., cv. Nipponbare), was examined. The Zn(2+)-dependent inhibition of the Al(3+)-induced oxidative burst was observed in both model cells selected from the monocots and dicots (rice and tobacco), suggesting that this phenomenon (Al(3+)/Zn(2+) interaction) can be preserved in higher plants. Subsequently induced cell death in tobacco cells was analyzed by lethal cell staining with Evans blue. Obtained results indicated that presence of Zn(2+) at physiological concentrations can protect the cells by preventing the Al(3+)-induced superoxide generation and cell death. Furthermore, the regulation of the Ca(2+) signaling, i.e., change in the cytosolic Ca(2+) ion concentration, and the cross-talks among the elements which participate in the pathway were further explored.

No MeSH data available.


Related in: MedlinePlus