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Treatment of a multiple sclerosis animal model by a novel nanodrop formulation of a natural antioxidant.

Binyamin O, Larush L, Frid K, Keller G, Friedman-Levi Y, Ovadia H, Abramsky O, Magdassi S, Gabizon R - Int J Nanomedicine (2015)

Bottom Line: Multiple sclerosis (MS) is a chronic inflammatory disease of the central nervous system and is associated with demyelination, neurodegeneration, and sensitivity to oxidative stress.Pathological examinations revealed that Nano-PSO administration dramatically reduced demyelination and oxidation of lipids in the brains of the affected animals, which are hallmarks of this severe neurological disease.On the mechanistic side, our results demonstrate that lipid oxidation may be a seminal feature in both demyelination and neurodegeneration.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, The Agnes Ginges Center of Human Neurogenetics, Hadassah University Hospital, Jerusalem, Israel.

ABSTRACT
Multiple sclerosis (MS) is a chronic inflammatory disease of the central nervous system and is associated with demyelination, neurodegeneration, and sensitivity to oxidative stress. In this work, we administered a nanodroplet formulation of pomegranate seed oil (PSO), denominated Nano-PSO, to mice induced for experimental autoimmune encephalomyelitis (EAE), an established model of MS. PSO comprises high levels of punicic acid, a unique polyunsaturated fatty acid considered as one of the strongest natural antioxidants. We show here that while EAE-induced mice treated with natural PSO presented some reduction in disease burden, this beneficial effect increased significantly when EAE mice were treated with Nano-PSO of specific size nanodroplets at much lower concentrations of the oil. Pathological examinations revealed that Nano-PSO administration dramatically reduced demyelination and oxidation of lipids in the brains of the affected animals, which are hallmarks of this severe neurological disease. We propose that novel formulations of natural antioxidants such as Nano-PSO may be considered for the treatment of patients suffering from demyelinating diseases. On the mechanistic side, our results demonstrate that lipid oxidation may be a seminal feature in both demyelination and neurodegeneration.

No MeSH data available.


Related in: MedlinePlus

TBARS levels in EAE brains.Notes: Samples from naïve, as well as treated and untreated EAE brains were subjected to the TBARS test.Abbreviations: TBARS, thiobarbituric acid reactive substances; EAE, experimental autoimmune encephalomyelitis; MDA, malonaldehyde.
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f7-ijn-10-7165: TBARS levels in EAE brains.Notes: Samples from naïve, as well as treated and untreated EAE brains were subjected to the TBARS test.Abbreviations: TBARS, thiobarbituric acid reactive substances; EAE, experimental autoimmune encephalomyelitis; MDA, malonaldehyde.

Mentions: MDA is a product of lipid peroxidation, and its levels can be empirically measured by the TBARS.40 It was recently shown to be increased in blood and saliva of MS patients.41 To establish whether MDA levels are elevated in the brains of EAE-induced mice and whether such elevation can be alleviated by Nano-PSO treatment, we subjected brain samples from six wild type, five EAE, and three treated EAE mice to the TBARS test (“Materials and methods” section). Figure 7 shows that while MDA levels are elevated significantly in the brains of EAE-induced mice (P<0.001 between naïve and untreated EAE brains), these are reduced almost to the levels of naïve brains following Nano-PSO treatment (P<0.663 between naïve and EAE-treated brains). These results, together with the results from Figure 6 also demonstrating reduced levels of oxidized phospholipids in Nano-PSO-treated EAE mice, strongly suggest that Nano-PSO may reduce lipid oxidation.


Treatment of a multiple sclerosis animal model by a novel nanodrop formulation of a natural antioxidant.

Binyamin O, Larush L, Frid K, Keller G, Friedman-Levi Y, Ovadia H, Abramsky O, Magdassi S, Gabizon R - Int J Nanomedicine (2015)

TBARS levels in EAE brains.Notes: Samples from naïve, as well as treated and untreated EAE brains were subjected to the TBARS test.Abbreviations: TBARS, thiobarbituric acid reactive substances; EAE, experimental autoimmune encephalomyelitis; MDA, malonaldehyde.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4664546&req=5

f7-ijn-10-7165: TBARS levels in EAE brains.Notes: Samples from naïve, as well as treated and untreated EAE brains were subjected to the TBARS test.Abbreviations: TBARS, thiobarbituric acid reactive substances; EAE, experimental autoimmune encephalomyelitis; MDA, malonaldehyde.
Mentions: MDA is a product of lipid peroxidation, and its levels can be empirically measured by the TBARS.40 It was recently shown to be increased in blood and saliva of MS patients.41 To establish whether MDA levels are elevated in the brains of EAE-induced mice and whether such elevation can be alleviated by Nano-PSO treatment, we subjected brain samples from six wild type, five EAE, and three treated EAE mice to the TBARS test (“Materials and methods” section). Figure 7 shows that while MDA levels are elevated significantly in the brains of EAE-induced mice (P<0.001 between naïve and untreated EAE brains), these are reduced almost to the levels of naïve brains following Nano-PSO treatment (P<0.663 between naïve and EAE-treated brains). These results, together with the results from Figure 6 also demonstrating reduced levels of oxidized phospholipids in Nano-PSO-treated EAE mice, strongly suggest that Nano-PSO may reduce lipid oxidation.

Bottom Line: Multiple sclerosis (MS) is a chronic inflammatory disease of the central nervous system and is associated with demyelination, neurodegeneration, and sensitivity to oxidative stress.Pathological examinations revealed that Nano-PSO administration dramatically reduced demyelination and oxidation of lipids in the brains of the affected animals, which are hallmarks of this severe neurological disease.On the mechanistic side, our results demonstrate that lipid oxidation may be a seminal feature in both demyelination and neurodegeneration.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, The Agnes Ginges Center of Human Neurogenetics, Hadassah University Hospital, Jerusalem, Israel.

ABSTRACT
Multiple sclerosis (MS) is a chronic inflammatory disease of the central nervous system and is associated with demyelination, neurodegeneration, and sensitivity to oxidative stress. In this work, we administered a nanodroplet formulation of pomegranate seed oil (PSO), denominated Nano-PSO, to mice induced for experimental autoimmune encephalomyelitis (EAE), an established model of MS. PSO comprises high levels of punicic acid, a unique polyunsaturated fatty acid considered as one of the strongest natural antioxidants. We show here that while EAE-induced mice treated with natural PSO presented some reduction in disease burden, this beneficial effect increased significantly when EAE mice were treated with Nano-PSO of specific size nanodroplets at much lower concentrations of the oil. Pathological examinations revealed that Nano-PSO administration dramatically reduced demyelination and oxidation of lipids in the brains of the affected animals, which are hallmarks of this severe neurological disease. We propose that novel formulations of natural antioxidants such as Nano-PSO may be considered for the treatment of patients suffering from demyelinating diseases. On the mechanistic side, our results demonstrate that lipid oxidation may be a seminal feature in both demyelination and neurodegeneration.

No MeSH data available.


Related in: MedlinePlus