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Mammalian Mss51 is a Skeletal Muscle-Specific Gene Modulating Cellular Metabolism

View Article: PubMed Central - PubMed

ABSTRACT

Background:: The transforming growth factor β (TGF-β) signaling pathways modulate skeletal muscle growth, regeneration, and cellular metabolism. Several recent gene expression studies have shown that inhibition of myostatin and TGF-β1 signaling consistently leads to a significant reduction in expression of Mss51, also named Zmynd17. The function of mammalian Mss51 is unknown although a putative homolog in yeast is a mitochondrial translational activator.

Objective:: The objective of this work was to characterize mammalian MSS51.

Methods:: Quantitative RT-PCR and immunoblot of subcellular fractionation were used to determine expression patterns and localization of Mss51. The CRISPR/Cas9 system was used to reduce expression of Mss51 in C2C12 myoblasts and the function of Mss51 was evaluated in assays of proliferation, differentiation and cellular metabolism.

Results:: Mss51 was predominantly expressed in skeletal muscle and in those muscles dominated by fast-twitch fibers. In vitro, its expression was upregulated upon differentiation of C2C12 myoblasts into myotubes. Expression of Mss51 was modulated in response to altered TGF-β family signaling. In human muscle, MSS51 localized to the mitochondria. Its genetic disruption resulted in increased levels of cellular ATP, β-oxidation, glycolysis, and oxidative phosphorylation.

Conclusions:: Mss51 is a novel, skeletal muscle-specific gene and a key target of myostatin and TGF-β1 signaling. Unlike myostatin, TGF-β1 and IGF-1, Mss51 does not regulate myoblast proliferation or differentiation. Rather, Mss51 appears to be one of the effectors of these growth factors on metabolic processes including fatty acid oxidation, glycolysis and oxidative phosphorylation.

No MeSH data available.


Subcellular localization of MSS51. Subcellular fractionation was performed on human deltoid samples from a biopsy and an autopsy and resulting fractions were subjected to SDS-PAGE. Immunoblotting was performed with the only current antibody specific to MSS51 (anti-human MSS51, Acris Antibodies, San Diego, CA, USA) showing a band in the mitochondrial fraction of the predicted protein product size, 51 kDa. Loading controls were VDAC (mitochondrial), GAPDH (cytoplasmic), and Histone H3 (nuclear).
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jnd-2-4-jnd150119-g003: Subcellular localization of MSS51. Subcellular fractionation was performed on human deltoid samples from a biopsy and an autopsy and resulting fractions were subjected to SDS-PAGE. Immunoblotting was performed with the only current antibody specific to MSS51 (anti-human MSS51, Acris Antibodies, San Diego, CA, USA) showing a band in the mitochondrial fraction of the predicted protein product size, 51 kDa. Loading controls were VDAC (mitochondrial), GAPDH (cytoplasmic), and Histone H3 (nuclear).

Mentions: To determine cellular localization of MSS51, human deltoid muscles from an open muscle biopsy of a living donor and from an autopsy were used to perform subcellular fractionation. Fractions of nuclear, cytoplasmic, and mitochondrial proteins were collected and used for SDS-PAGE and Western blotting. These data showed that MSS51 protein co-fractionated with the mitochondrial fraction (Fig. 3).


Mammalian Mss51 is a Skeletal Muscle-Specific Gene Modulating Cellular Metabolism
Subcellular localization of MSS51. Subcellular fractionation was performed on human deltoid samples from a biopsy and an autopsy and resulting fractions were subjected to SDS-PAGE. Immunoblotting was performed with the only current antibody specific to MSS51 (anti-human MSS51, Acris Antibodies, San Diego, CA, USA) showing a band in the mitochondrial fraction of the predicted protein product size, 51 kDa. Loading controls were VDAC (mitochondrial), GAPDH (cytoplasmic), and Histone H3 (nuclear).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4664537&req=5

jnd-2-4-jnd150119-g003: Subcellular localization of MSS51. Subcellular fractionation was performed on human deltoid samples from a biopsy and an autopsy and resulting fractions were subjected to SDS-PAGE. Immunoblotting was performed with the only current antibody specific to MSS51 (anti-human MSS51, Acris Antibodies, San Diego, CA, USA) showing a band in the mitochondrial fraction of the predicted protein product size, 51 kDa. Loading controls were VDAC (mitochondrial), GAPDH (cytoplasmic), and Histone H3 (nuclear).
Mentions: To determine cellular localization of MSS51, human deltoid muscles from an open muscle biopsy of a living donor and from an autopsy were used to perform subcellular fractionation. Fractions of nuclear, cytoplasmic, and mitochondrial proteins were collected and used for SDS-PAGE and Western blotting. These data showed that MSS51 protein co-fractionated with the mitochondrial fraction (Fig. 3).

View Article: PubMed Central - PubMed

ABSTRACT

Background:: The transforming growth factor β (TGF-β) signaling pathways modulate skeletal muscle growth, regeneration, and cellular metabolism. Several recent gene expression studies have shown that inhibition of myostatin and TGF-β1 signaling consistently leads to a significant reduction in expression of Mss51, also named Zmynd17. The function of mammalian Mss51 is unknown although a putative homolog in yeast is a mitochondrial translational activator.

Objective:: The objective of this work was to characterize mammalian MSS51.

Methods:: Quantitative RT-PCR and immunoblot of subcellular fractionation were used to determine expression patterns and localization of Mss51. The CRISPR/Cas9 system was used to reduce expression of Mss51 in C2C12 myoblasts and the function of Mss51 was evaluated in assays of proliferation, differentiation and cellular metabolism.

Results:: Mss51 was predominantly expressed in skeletal muscle and in those muscles dominated by fast-twitch fibers. In vitro, its expression was upregulated upon differentiation of C2C12 myoblasts into myotubes. Expression of Mss51 was modulated in response to altered TGF-β family signaling. In human muscle, MSS51 localized to the mitochondria. Its genetic disruption resulted in increased levels of cellular ATP, β-oxidation, glycolysis, and oxidative phosphorylation.

Conclusions:: Mss51 is a novel, skeletal muscle-specific gene and a key target of myostatin and TGF-β1 signaling. Unlike myostatin, TGF-β1 and IGF-1, Mss51 does not regulate myoblast proliferation or differentiation. Rather, Mss51 appears to be one of the effectors of these growth factors on metabolic processes including fatty acid oxidation, glycolysis and oxidative phosphorylation.

No MeSH data available.