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Assessing the axonal translocation of CeO2 and SiO2 nanoparticles in the sciatic nerve fibers of the frog: an ex vivo electrophysiological study.

Kastrinaki G, Samsouris C, Kosmidis EK, Papaioannou E, Konstandopoulos AG, Theophilidis G - Int J Nanomedicine (2015)

Bottom Line: For the CeO2, we also demonstrated that the translocation depends on both axonal integrity and electrical activity.The speed of translocation for the two species was estimated in the range of 0.45-0.58 mm/h, close to slow axonal transportation rate.Transmission electron microscopy provided direct evidence for the presence of SiO2 in the treated nerves.

View Article: PubMed Central - PubMed

Affiliation: Aerosol and Particle Technology Laboratory (APTL), CERTH/CPERI, Thessaloniki, Greece.

ABSTRACT
The axonal translocation of two commonly used nanoparticles in medicine, namely CeO2 and SiO2, is investigated. The study was conducted on frog sciatic nerve fibers in an ex vivo preparation. Nanoparticles were applied at the proximal end of the excised nerve. A nerve stimulation protocol was followed for over 35 hours. Nerve vitality curve comparison between control and exposed nerves showed that CeO2 has no neurotoxic effect at the concentrations tested. After exposure, specimens were fixed and then screen scanned every 1 mm along their length for nanoparticle presence by means of Fourier transform infrared microscopy. We demonstrated that both nanoparticles translocate within the nerve by formation of narrow bands in the Fourier transform infrared spectrum. For the CeO2, we also demonstrated that the translocation depends on both axonal integrity and electrical activity. The speed of translocation for the two species was estimated in the range of 0.45-0.58 mm/h, close to slow axonal transportation rate. Transmission electron microscopy provided direct evidence for the presence of SiO2 in the treated nerves.

No MeSH data available.


Related in: MedlinePlus

TEM image of the teased and dried nerve fibers, depicting a SiO2 NP.Abbreviations: NP, nanoparticle; TEM, transmission electron microscope.
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f5-ijn-10-7089: TEM image of the teased and dried nerve fibers, depicting a SiO2 NP.Abbreviations: NP, nanoparticle; TEM, transmission electron microscope.

Mentions: To provide convincing evidence that our FTIR signal was generated by SiO2 NPs, parts of the nerve with strong signal were examined using TEM. A SiO2 NP from these studies is shown in Figure 5. The TEM images depict spherical dense particle morphology with diameter size distribution between 30 and 120 nm and amorphous crystallinity, all concordant with SiO2 presence.


Assessing the axonal translocation of CeO2 and SiO2 nanoparticles in the sciatic nerve fibers of the frog: an ex vivo electrophysiological study.

Kastrinaki G, Samsouris C, Kosmidis EK, Papaioannou E, Konstandopoulos AG, Theophilidis G - Int J Nanomedicine (2015)

TEM image of the teased and dried nerve fibers, depicting a SiO2 NP.Abbreviations: NP, nanoparticle; TEM, transmission electron microscope.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4664516&req=5

f5-ijn-10-7089: TEM image of the teased and dried nerve fibers, depicting a SiO2 NP.Abbreviations: NP, nanoparticle; TEM, transmission electron microscope.
Mentions: To provide convincing evidence that our FTIR signal was generated by SiO2 NPs, parts of the nerve with strong signal were examined using TEM. A SiO2 NP from these studies is shown in Figure 5. The TEM images depict spherical dense particle morphology with diameter size distribution between 30 and 120 nm and amorphous crystallinity, all concordant with SiO2 presence.

Bottom Line: For the CeO2, we also demonstrated that the translocation depends on both axonal integrity and electrical activity.The speed of translocation for the two species was estimated in the range of 0.45-0.58 mm/h, close to slow axonal transportation rate.Transmission electron microscopy provided direct evidence for the presence of SiO2 in the treated nerves.

View Article: PubMed Central - PubMed

Affiliation: Aerosol and Particle Technology Laboratory (APTL), CERTH/CPERI, Thessaloniki, Greece.

ABSTRACT
The axonal translocation of two commonly used nanoparticles in medicine, namely CeO2 and SiO2, is investigated. The study was conducted on frog sciatic nerve fibers in an ex vivo preparation. Nanoparticles were applied at the proximal end of the excised nerve. A nerve stimulation protocol was followed for over 35 hours. Nerve vitality curve comparison between control and exposed nerves showed that CeO2 has no neurotoxic effect at the concentrations tested. After exposure, specimens were fixed and then screen scanned every 1 mm along their length for nanoparticle presence by means of Fourier transform infrared microscopy. We demonstrated that both nanoparticles translocate within the nerve by formation of narrow bands in the Fourier transform infrared spectrum. For the CeO2, we also demonstrated that the translocation depends on both axonal integrity and electrical activity. The speed of translocation for the two species was estimated in the range of 0.45-0.58 mm/h, close to slow axonal transportation rate. Transmission electron microscopy provided direct evidence for the presence of SiO2 in the treated nerves.

No MeSH data available.


Related in: MedlinePlus