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Assessing the axonal translocation of CeO2 and SiO2 nanoparticles in the sciatic nerve fibers of the frog: an ex vivo electrophysiological study.

Kastrinaki G, Samsouris C, Kosmidis EK, Papaioannou E, Konstandopoulos AG, Theophilidis G - Int J Nanomedicine (2015)

Bottom Line: For the CeO2, we also demonstrated that the translocation depends on both axonal integrity and electrical activity.The speed of translocation for the two species was estimated in the range of 0.45-0.58 mm/h, close to slow axonal transportation rate.Transmission electron microscopy provided direct evidence for the presence of SiO2 in the treated nerves.

View Article: PubMed Central - PubMed

Affiliation: Aerosol and Particle Technology Laboratory (APTL), CERTH/CPERI, Thessaloniki, Greece.

ABSTRACT
The axonal translocation of two commonly used nanoparticles in medicine, namely CeO2 and SiO2, is investigated. The study was conducted on frog sciatic nerve fibers in an ex vivo preparation. Nanoparticles were applied at the proximal end of the excised nerve. A nerve stimulation protocol was followed for over 35 hours. Nerve vitality curve comparison between control and exposed nerves showed that CeO2 has no neurotoxic effect at the concentrations tested. After exposure, specimens were fixed and then screen scanned every 1 mm along their length for nanoparticle presence by means of Fourier transform infrared microscopy. We demonstrated that both nanoparticles translocate within the nerve by formation of narrow bands in the Fourier transform infrared spectrum. For the CeO2, we also demonstrated that the translocation depends on both axonal integrity and electrical activity. The speed of translocation for the two species was estimated in the range of 0.45-0.58 mm/h, close to slow axonal transportation rate. Transmission electron microscopy provided direct evidence for the presence of SiO2 in the treated nerves.

No MeSH data available.


Related in: MedlinePlus

FTIR traces of different SiO2 treated nerve regions.Notes: Arrows indicate SiO2 presence. Peaks approximately 1,640 cm−1 are attributed to the ν2′ band of the hydrogen bond of water.Abbreviations: FTIR, Fourier transform infrared microscopy; NP, nanoparticle.
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f4-ijn-10-7089: FTIR traces of different SiO2 treated nerve regions.Notes: Arrows indicate SiO2 presence. Peaks approximately 1,640 cm−1 are attributed to the ν2′ band of the hydrogen bond of water.Abbreviations: FTIR, Fourier transform infrared microscopy; NP, nanoparticle.

Mentions: In Figure 4, the FTIR traces of a nonexposed nerve (control nerve) and of the SiO2 NPs are shown as negative and positive controls, respectively. No NPs were detected in the middle and distal parts of the nerve. However, in the distal part, 5 mm before the end of the nerve, an area with high SiO2 presence was detected (trace high SiO2) following a low one (trace low SiO2). Prior to this region, and similar to the other areas of the nerve, NPs were not detected (trace no SiO2).


Assessing the axonal translocation of CeO2 and SiO2 nanoparticles in the sciatic nerve fibers of the frog: an ex vivo electrophysiological study.

Kastrinaki G, Samsouris C, Kosmidis EK, Papaioannou E, Konstandopoulos AG, Theophilidis G - Int J Nanomedicine (2015)

FTIR traces of different SiO2 treated nerve regions.Notes: Arrows indicate SiO2 presence. Peaks approximately 1,640 cm−1 are attributed to the ν2′ band of the hydrogen bond of water.Abbreviations: FTIR, Fourier transform infrared microscopy; NP, nanoparticle.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4664516&req=5

f4-ijn-10-7089: FTIR traces of different SiO2 treated nerve regions.Notes: Arrows indicate SiO2 presence. Peaks approximately 1,640 cm−1 are attributed to the ν2′ band of the hydrogen bond of water.Abbreviations: FTIR, Fourier transform infrared microscopy; NP, nanoparticle.
Mentions: In Figure 4, the FTIR traces of a nonexposed nerve (control nerve) and of the SiO2 NPs are shown as negative and positive controls, respectively. No NPs were detected in the middle and distal parts of the nerve. However, in the distal part, 5 mm before the end of the nerve, an area with high SiO2 presence was detected (trace high SiO2) following a low one (trace low SiO2). Prior to this region, and similar to the other areas of the nerve, NPs were not detected (trace no SiO2).

Bottom Line: For the CeO2, we also demonstrated that the translocation depends on both axonal integrity and electrical activity.The speed of translocation for the two species was estimated in the range of 0.45-0.58 mm/h, close to slow axonal transportation rate.Transmission electron microscopy provided direct evidence for the presence of SiO2 in the treated nerves.

View Article: PubMed Central - PubMed

Affiliation: Aerosol and Particle Technology Laboratory (APTL), CERTH/CPERI, Thessaloniki, Greece.

ABSTRACT
The axonal translocation of two commonly used nanoparticles in medicine, namely CeO2 and SiO2, is investigated. The study was conducted on frog sciatic nerve fibers in an ex vivo preparation. Nanoparticles were applied at the proximal end of the excised nerve. A nerve stimulation protocol was followed for over 35 hours. Nerve vitality curve comparison between control and exposed nerves showed that CeO2 has no neurotoxic effect at the concentrations tested. After exposure, specimens were fixed and then screen scanned every 1 mm along their length for nanoparticle presence by means of Fourier transform infrared microscopy. We demonstrated that both nanoparticles translocate within the nerve by formation of narrow bands in the Fourier transform infrared spectrum. For the CeO2, we also demonstrated that the translocation depends on both axonal integrity and electrical activity. The speed of translocation for the two species was estimated in the range of 0.45-0.58 mm/h, close to slow axonal transportation rate. Transmission electron microscopy provided direct evidence for the presence of SiO2 in the treated nerves.

No MeSH data available.


Related in: MedlinePlus