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Effect of gyromagnetic fields on human prostatic adenocarcinoma cells.

Lei H, Xu Y, Guan R, Li M, Hui Y, Gao Z, Yang B, Xin Z - Onco Targets Ther (2015)

Bottom Line: PC-3 cells were grouped into normal control (NC) and GMF treatment groups.Cell proliferation and activity of the Akt pathway were significantly decreased by the GMF, while cell apoptosis, activity of p38 MAPK, and PARD3-positive cell number were significantly increased in the GMF group compared to the NC group.GMFs inhibit cell proliferation, induce apoptosis, and regulate tumor cell polarity conditions, potentially through down-regulating Akt, activating the p38 MAPK/Caspase pathway, and promoting PARD3 expression in PC-3 cells.

View Article: PubMed Central - PubMed

Affiliation: Andrology Center, Peking University First Hospital, Peking University, Beijing, People's Republic of China.

ABSTRACT

Purpose: To investigate the biological effect of gyromagnetic fields (GMFs) on cell proliferation and apoptosis of human prostatic adenocarcinoma cells and explore the underlying mechanisms.

Methods: PC-3 cells were grouped into normal control (NC) and GMF treatment groups. Cell proliferation was analyzed with kit-8 and Ki67 immunofluorescence staining, while cell apoptosis was analyzed with flow cytometry double staining of Annexin V-PE/7-AAD. The Akt and p38 MAPK/Caspase signaling pathways were analyzed by western blotting and immunofluorescence staining, and cell polarization was analyzed with PARD3.

Results: Cell proliferation and activity of the Akt pathway were significantly decreased by the GMF, while cell apoptosis, activity of p38 MAPK, and PARD3-positive cell number were significantly increased in the GMF group compared to the NC group.

Conclusion: GMFs inhibit cell proliferation, induce apoptosis, and regulate tumor cell polarity conditions, potentially through down-regulating Akt, activating the p38 MAPK/Caspase pathway, and promoting PARD3 expression in PC-3 cells.

No MeSH data available.


Related in: MedlinePlus

Effect of gyromagnetic fields on PARD3 protein in PC-3 cells.Notes: (A) PARD3 (red) immunofluorescence staining in PC-3 cells 24 hours after treatment with a gyromagnetic field from the normal control (NC group) and cells treated once with a magnetic field for 10 minutes (GMF 10 minutes). Nuclei were labeled with 4′,6-diamidino-2-phenylindole (DAPI) (blue). Scale bars =50 μm, 200×. (B) Quantitative data of PARD3-positive cell number per high power field (HPF) by Image-Pro plus (n=3). *P<0.05 when compared with the NC group.
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f6-ott-8-3489: Effect of gyromagnetic fields on PARD3 protein in PC-3 cells.Notes: (A) PARD3 (red) immunofluorescence staining in PC-3 cells 24 hours after treatment with a gyromagnetic field from the normal control (NC group) and cells treated once with a magnetic field for 10 minutes (GMF 10 minutes). Nuclei were labeled with 4′,6-diamidino-2-phenylindole (DAPI) (blue). Scale bars =50 μm, 200×. (B) Quantitative data of PARD3-positive cell number per high power field (HPF) by Image-Pro plus (n=3). *P<0.05 when compared with the NC group.

Mentions: To demonstrate the effects of GMFs on cell polarity regulation, immunofluorescence staining of PARD3, a crucial regulator of cell polarization and tumorigenesis, was detected 24 hours after treatment with a GMF. PARD3-positive cell number was significantly increased in the GMF 10 minutes group when compared with the NC group (P<0.05; Figure 6). The data suggested that abnormal cell polarity conditions of tumor cells were regulated by GMFs in PC-3 cells.


Effect of gyromagnetic fields on human prostatic adenocarcinoma cells.

Lei H, Xu Y, Guan R, Li M, Hui Y, Gao Z, Yang B, Xin Z - Onco Targets Ther (2015)

Effect of gyromagnetic fields on PARD3 protein in PC-3 cells.Notes: (A) PARD3 (red) immunofluorescence staining in PC-3 cells 24 hours after treatment with a gyromagnetic field from the normal control (NC group) and cells treated once with a magnetic field for 10 minutes (GMF 10 minutes). Nuclei were labeled with 4′,6-diamidino-2-phenylindole (DAPI) (blue). Scale bars =50 μm, 200×. (B) Quantitative data of PARD3-positive cell number per high power field (HPF) by Image-Pro plus (n=3). *P<0.05 when compared with the NC group.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4664505&req=5

f6-ott-8-3489: Effect of gyromagnetic fields on PARD3 protein in PC-3 cells.Notes: (A) PARD3 (red) immunofluorescence staining in PC-3 cells 24 hours after treatment with a gyromagnetic field from the normal control (NC group) and cells treated once with a magnetic field for 10 minutes (GMF 10 minutes). Nuclei were labeled with 4′,6-diamidino-2-phenylindole (DAPI) (blue). Scale bars =50 μm, 200×. (B) Quantitative data of PARD3-positive cell number per high power field (HPF) by Image-Pro plus (n=3). *P<0.05 when compared with the NC group.
Mentions: To demonstrate the effects of GMFs on cell polarity regulation, immunofluorescence staining of PARD3, a crucial regulator of cell polarization and tumorigenesis, was detected 24 hours after treatment with a GMF. PARD3-positive cell number was significantly increased in the GMF 10 minutes group when compared with the NC group (P<0.05; Figure 6). The data suggested that abnormal cell polarity conditions of tumor cells were regulated by GMFs in PC-3 cells.

Bottom Line: PC-3 cells were grouped into normal control (NC) and GMF treatment groups.Cell proliferation and activity of the Akt pathway were significantly decreased by the GMF, while cell apoptosis, activity of p38 MAPK, and PARD3-positive cell number were significantly increased in the GMF group compared to the NC group.GMFs inhibit cell proliferation, induce apoptosis, and regulate tumor cell polarity conditions, potentially through down-regulating Akt, activating the p38 MAPK/Caspase pathway, and promoting PARD3 expression in PC-3 cells.

View Article: PubMed Central - PubMed

Affiliation: Andrology Center, Peking University First Hospital, Peking University, Beijing, People's Republic of China.

ABSTRACT

Purpose: To investigate the biological effect of gyromagnetic fields (GMFs) on cell proliferation and apoptosis of human prostatic adenocarcinoma cells and explore the underlying mechanisms.

Methods: PC-3 cells were grouped into normal control (NC) and GMF treatment groups. Cell proliferation was analyzed with kit-8 and Ki67 immunofluorescence staining, while cell apoptosis was analyzed with flow cytometry double staining of Annexin V-PE/7-AAD. The Akt and p38 MAPK/Caspase signaling pathways were analyzed by western blotting and immunofluorescence staining, and cell polarization was analyzed with PARD3.

Results: Cell proliferation and activity of the Akt pathway were significantly decreased by the GMF, while cell apoptosis, activity of p38 MAPK, and PARD3-positive cell number were significantly increased in the GMF group compared to the NC group.

Conclusion: GMFs inhibit cell proliferation, induce apoptosis, and regulate tumor cell polarity conditions, potentially through down-regulating Akt, activating the p38 MAPK/Caspase pathway, and promoting PARD3 expression in PC-3 cells.

No MeSH data available.


Related in: MedlinePlus