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Effect of gyromagnetic fields on human prostatic adenocarcinoma cells.

Lei H, Xu Y, Guan R, Li M, Hui Y, Gao Z, Yang B, Xin Z - Onco Targets Ther (2015)

Bottom Line: PC-3 cells were grouped into normal control (NC) and GMF treatment groups.Cell proliferation and activity of the Akt pathway were significantly decreased by the GMF, while cell apoptosis, activity of p38 MAPK, and PARD3-positive cell number were significantly increased in the GMF group compared to the NC group.GMFs inhibit cell proliferation, induce apoptosis, and regulate tumor cell polarity conditions, potentially through down-regulating Akt, activating the p38 MAPK/Caspase pathway, and promoting PARD3 expression in PC-3 cells.

View Article: PubMed Central - PubMed

Affiliation: Andrology Center, Peking University First Hospital, Peking University, Beijing, People's Republic of China.

ABSTRACT

Purpose: To investigate the biological effect of gyromagnetic fields (GMFs) on cell proliferation and apoptosis of human prostatic adenocarcinoma cells and explore the underlying mechanisms.

Methods: PC-3 cells were grouped into normal control (NC) and GMF treatment groups. Cell proliferation was analyzed with kit-8 and Ki67 immunofluorescence staining, while cell apoptosis was analyzed with flow cytometry double staining of Annexin V-PE/7-AAD. The Akt and p38 MAPK/Caspase signaling pathways were analyzed by western blotting and immunofluorescence staining, and cell polarization was analyzed with PARD3.

Results: Cell proliferation and activity of the Akt pathway were significantly decreased by the GMF, while cell apoptosis, activity of p38 MAPK, and PARD3-positive cell number were significantly increased in the GMF group compared to the NC group.

Conclusion: GMFs inhibit cell proliferation, induce apoptosis, and regulate tumor cell polarity conditions, potentially through down-regulating Akt, activating the p38 MAPK/Caspase pathway, and promoting PARD3 expression in PC-3 cells.

No MeSH data available.


Related in: MedlinePlus

Effect of gyromagnetic fields on p38 MAPK/Caspase in PC-3 cells.Notes: (A) Western blot analysis for phospho-p38 MAPK, p38 MAPK, and Cleaved Caspase-9 expression in PC-3 cells 24 hours after treatment with a gyromagnetic field from the normal control (NC) group and the gyromagnetic field (GMF) groups with treatment times of 1, 5, 10, and 20 minutes. (B and C) Quantitative data of band intensity for phospho-p38 MAPK, p38 MAPK, and Cleaved Caspase-9 by ImageJ (n=3). (D) Cleaved Caspase-3 (red) immunofluorescence staining in PC-3 cells at 24 hours after treatment with a gyromagnetic field from the NC group and cells treated once with a magnetic field for 10 minutes (GMF 10 minutes). Nuclei were labeled with 4′,6-diamidino-2-phenylindole (DAPI) (blue). Scale bars =50 μm, 200×. (E) Quantitative data of Cleaved Caspase-3-positive cell number per high power field (HPF) by Image-Pro plus (n=3). *P<0.05 when compared with the NC group.
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f5-ott-8-3489: Effect of gyromagnetic fields on p38 MAPK/Caspase in PC-3 cells.Notes: (A) Western blot analysis for phospho-p38 MAPK, p38 MAPK, and Cleaved Caspase-9 expression in PC-3 cells 24 hours after treatment with a gyromagnetic field from the normal control (NC) group and the gyromagnetic field (GMF) groups with treatment times of 1, 5, 10, and 20 minutes. (B and C) Quantitative data of band intensity for phospho-p38 MAPK, p38 MAPK, and Cleaved Caspase-9 by ImageJ (n=3). (D) Cleaved Caspase-3 (red) immunofluorescence staining in PC-3 cells at 24 hours after treatment with a gyromagnetic field from the NC group and cells treated once with a magnetic field for 10 minutes (GMF 10 minutes). Nuclei were labeled with 4′,6-diamidino-2-phenylindole (DAPI) (blue). Scale bars =50 μm, 200×. (E) Quantitative data of Cleaved Caspase-3-positive cell number per high power field (HPF) by Image-Pro plus (n=3). *P<0.05 when compared with the NC group.

Mentions: To understand the mechanism of the effects of GMFs on cell apoptosis, expression levels of proteins in apoptosis associated p38 MAPK/Caspase signaling were detected. Twenty-four hours after GMF treatment, western blot analysis showed that phospho-p38 MAPK/p38 MAPK ratio and Cleaved Caspase-9 expression levels were significantly increased in PC-3 cells (P<0.05; Figure 5A–C), and also immunofluorescence staining showed that Cleaved Caspase-3-positive cell numbers were significantly increased in the GMF 10-minute group (P<0.05; Figure 5D and E). These results indicated that p38 MAPK/Caspase signaling was activated under exposure to GMFs.


Effect of gyromagnetic fields on human prostatic adenocarcinoma cells.

Lei H, Xu Y, Guan R, Li M, Hui Y, Gao Z, Yang B, Xin Z - Onco Targets Ther (2015)

Effect of gyromagnetic fields on p38 MAPK/Caspase in PC-3 cells.Notes: (A) Western blot analysis for phospho-p38 MAPK, p38 MAPK, and Cleaved Caspase-9 expression in PC-3 cells 24 hours after treatment with a gyromagnetic field from the normal control (NC) group and the gyromagnetic field (GMF) groups with treatment times of 1, 5, 10, and 20 minutes. (B and C) Quantitative data of band intensity for phospho-p38 MAPK, p38 MAPK, and Cleaved Caspase-9 by ImageJ (n=3). (D) Cleaved Caspase-3 (red) immunofluorescence staining in PC-3 cells at 24 hours after treatment with a gyromagnetic field from the NC group and cells treated once with a magnetic field for 10 minutes (GMF 10 minutes). Nuclei were labeled with 4′,6-diamidino-2-phenylindole (DAPI) (blue). Scale bars =50 μm, 200×. (E) Quantitative data of Cleaved Caspase-3-positive cell number per high power field (HPF) by Image-Pro plus (n=3). *P<0.05 when compared with the NC group.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4664505&req=5

f5-ott-8-3489: Effect of gyromagnetic fields on p38 MAPK/Caspase in PC-3 cells.Notes: (A) Western blot analysis for phospho-p38 MAPK, p38 MAPK, and Cleaved Caspase-9 expression in PC-3 cells 24 hours after treatment with a gyromagnetic field from the normal control (NC) group and the gyromagnetic field (GMF) groups with treatment times of 1, 5, 10, and 20 minutes. (B and C) Quantitative data of band intensity for phospho-p38 MAPK, p38 MAPK, and Cleaved Caspase-9 by ImageJ (n=3). (D) Cleaved Caspase-3 (red) immunofluorescence staining in PC-3 cells at 24 hours after treatment with a gyromagnetic field from the NC group and cells treated once with a magnetic field for 10 minutes (GMF 10 minutes). Nuclei were labeled with 4′,6-diamidino-2-phenylindole (DAPI) (blue). Scale bars =50 μm, 200×. (E) Quantitative data of Cleaved Caspase-3-positive cell number per high power field (HPF) by Image-Pro plus (n=3). *P<0.05 when compared with the NC group.
Mentions: To understand the mechanism of the effects of GMFs on cell apoptosis, expression levels of proteins in apoptosis associated p38 MAPK/Caspase signaling were detected. Twenty-four hours after GMF treatment, western blot analysis showed that phospho-p38 MAPK/p38 MAPK ratio and Cleaved Caspase-9 expression levels were significantly increased in PC-3 cells (P<0.05; Figure 5A–C), and also immunofluorescence staining showed that Cleaved Caspase-3-positive cell numbers were significantly increased in the GMF 10-minute group (P<0.05; Figure 5D and E). These results indicated that p38 MAPK/Caspase signaling was activated under exposure to GMFs.

Bottom Line: PC-3 cells were grouped into normal control (NC) and GMF treatment groups.Cell proliferation and activity of the Akt pathway were significantly decreased by the GMF, while cell apoptosis, activity of p38 MAPK, and PARD3-positive cell number were significantly increased in the GMF group compared to the NC group.GMFs inhibit cell proliferation, induce apoptosis, and regulate tumor cell polarity conditions, potentially through down-regulating Akt, activating the p38 MAPK/Caspase pathway, and promoting PARD3 expression in PC-3 cells.

View Article: PubMed Central - PubMed

Affiliation: Andrology Center, Peking University First Hospital, Peking University, Beijing, People's Republic of China.

ABSTRACT

Purpose: To investigate the biological effect of gyromagnetic fields (GMFs) on cell proliferation and apoptosis of human prostatic adenocarcinoma cells and explore the underlying mechanisms.

Methods: PC-3 cells were grouped into normal control (NC) and GMF treatment groups. Cell proliferation was analyzed with kit-8 and Ki67 immunofluorescence staining, while cell apoptosis was analyzed with flow cytometry double staining of Annexin V-PE/7-AAD. The Akt and p38 MAPK/Caspase signaling pathways were analyzed by western blotting and immunofluorescence staining, and cell polarization was analyzed with PARD3.

Results: Cell proliferation and activity of the Akt pathway were significantly decreased by the GMF, while cell apoptosis, activity of p38 MAPK, and PARD3-positive cell number were significantly increased in the GMF group compared to the NC group.

Conclusion: GMFs inhibit cell proliferation, induce apoptosis, and regulate tumor cell polarity conditions, potentially through down-regulating Akt, activating the p38 MAPK/Caspase pathway, and promoting PARD3 expression in PC-3 cells.

No MeSH data available.


Related in: MedlinePlus