Limits...
Effect of gyromagnetic fields on human prostatic adenocarcinoma cells.

Lei H, Xu Y, Guan R, Li M, Hui Y, Gao Z, Yang B, Xin Z - Onco Targets Ther (2015)

Bottom Line: PC-3 cells were grouped into normal control (NC) and GMF treatment groups.Cell proliferation and activity of the Akt pathway were significantly decreased by the GMF, while cell apoptosis, activity of p38 MAPK, and PARD3-positive cell number were significantly increased in the GMF group compared to the NC group.GMFs inhibit cell proliferation, induce apoptosis, and regulate tumor cell polarity conditions, potentially through down-regulating Akt, activating the p38 MAPK/Caspase pathway, and promoting PARD3 expression in PC-3 cells.

View Article: PubMed Central - PubMed

Affiliation: Andrology Center, Peking University First Hospital, Peking University, Beijing, People's Republic of China.

ABSTRACT

Purpose: To investigate the biological effect of gyromagnetic fields (GMFs) on cell proliferation and apoptosis of human prostatic adenocarcinoma cells and explore the underlying mechanisms.

Methods: PC-3 cells were grouped into normal control (NC) and GMF treatment groups. Cell proliferation was analyzed with kit-8 and Ki67 immunofluorescence staining, while cell apoptosis was analyzed with flow cytometry double staining of Annexin V-PE/7-AAD. The Akt and p38 MAPK/Caspase signaling pathways were analyzed by western blotting and immunofluorescence staining, and cell polarization was analyzed with PARD3.

Results: Cell proliferation and activity of the Akt pathway were significantly decreased by the GMF, while cell apoptosis, activity of p38 MAPK, and PARD3-positive cell number were significantly increased in the GMF group compared to the NC group.

Conclusion: GMFs inhibit cell proliferation, induce apoptosis, and regulate tumor cell polarity conditions, potentially through down-regulating Akt, activating the p38 MAPK/Caspase pathway, and promoting PARD3 expression in PC-3 cells.

No MeSH data available.


Related in: MedlinePlus

Effect of gyromagnetic fields on cell apoptosis in PC-3 cells.Notes: (A) Flow cytometry analysis using an Annexin V-PE/7-AAD Apoptosis Detection Kit on PC-3 cells 24 hours after treatment with a gyromagnetic field from the normal control (NC group) and the gyromagnetic field (GMF) groups with treatment times of 1, 5, 10, and 20 minutes. (B) Quantitative data of cell apoptosis rate by FlowJO (n=3). Cells in late apoptosis were both Annexin V-PE positive and 7-AAD positive (Q2), and cells in early apoptosis were Annexin V-PE positive and 7-AAD negative (Q3). *P<0.05 when compared with the NC group.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4664505&req=5

f4-ott-8-3489: Effect of gyromagnetic fields on cell apoptosis in PC-3 cells.Notes: (A) Flow cytometry analysis using an Annexin V-PE/7-AAD Apoptosis Detection Kit on PC-3 cells 24 hours after treatment with a gyromagnetic field from the normal control (NC group) and the gyromagnetic field (GMF) groups with treatment times of 1, 5, 10, and 20 minutes. (B) Quantitative data of cell apoptosis rate by FlowJO (n=3). Cells in late apoptosis were both Annexin V-PE positive and 7-AAD positive (Q2), and cells in early apoptosis were Annexin V-PE positive and 7-AAD negative (Q3). *P<0.05 when compared with the NC group.

Mentions: To investigate if GMFs have an impact on cell apoptosis in PC-3 cells, we elucidated the rate of cell apoptosis by performing flow cytometry assays. Twenty-four hours after the influence of a GMF, late apoptotic cell rate was significantly increased in the GMF groups (P<0.05), while no significant differences were observed on the early apoptotic cell rate among groups (P>0.05; Figure 4). The data suggested that GMFs may have an effect of inducing cell apoptosis in PC-3 cells.


Effect of gyromagnetic fields on human prostatic adenocarcinoma cells.

Lei H, Xu Y, Guan R, Li M, Hui Y, Gao Z, Yang B, Xin Z - Onco Targets Ther (2015)

Effect of gyromagnetic fields on cell apoptosis in PC-3 cells.Notes: (A) Flow cytometry analysis using an Annexin V-PE/7-AAD Apoptosis Detection Kit on PC-3 cells 24 hours after treatment with a gyromagnetic field from the normal control (NC group) and the gyromagnetic field (GMF) groups with treatment times of 1, 5, 10, and 20 minutes. (B) Quantitative data of cell apoptosis rate by FlowJO (n=3). Cells in late apoptosis were both Annexin V-PE positive and 7-AAD positive (Q2), and cells in early apoptosis were Annexin V-PE positive and 7-AAD negative (Q3). *P<0.05 when compared with the NC group.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4664505&req=5

f4-ott-8-3489: Effect of gyromagnetic fields on cell apoptosis in PC-3 cells.Notes: (A) Flow cytometry analysis using an Annexin V-PE/7-AAD Apoptosis Detection Kit on PC-3 cells 24 hours after treatment with a gyromagnetic field from the normal control (NC group) and the gyromagnetic field (GMF) groups with treatment times of 1, 5, 10, and 20 minutes. (B) Quantitative data of cell apoptosis rate by FlowJO (n=3). Cells in late apoptosis were both Annexin V-PE positive and 7-AAD positive (Q2), and cells in early apoptosis were Annexin V-PE positive and 7-AAD negative (Q3). *P<0.05 when compared with the NC group.
Mentions: To investigate if GMFs have an impact on cell apoptosis in PC-3 cells, we elucidated the rate of cell apoptosis by performing flow cytometry assays. Twenty-four hours after the influence of a GMF, late apoptotic cell rate was significantly increased in the GMF groups (P<0.05), while no significant differences were observed on the early apoptotic cell rate among groups (P>0.05; Figure 4). The data suggested that GMFs may have an effect of inducing cell apoptosis in PC-3 cells.

Bottom Line: PC-3 cells were grouped into normal control (NC) and GMF treatment groups.Cell proliferation and activity of the Akt pathway were significantly decreased by the GMF, while cell apoptosis, activity of p38 MAPK, and PARD3-positive cell number were significantly increased in the GMF group compared to the NC group.GMFs inhibit cell proliferation, induce apoptosis, and regulate tumor cell polarity conditions, potentially through down-regulating Akt, activating the p38 MAPK/Caspase pathway, and promoting PARD3 expression in PC-3 cells.

View Article: PubMed Central - PubMed

Affiliation: Andrology Center, Peking University First Hospital, Peking University, Beijing, People's Republic of China.

ABSTRACT

Purpose: To investigate the biological effect of gyromagnetic fields (GMFs) on cell proliferation and apoptosis of human prostatic adenocarcinoma cells and explore the underlying mechanisms.

Methods: PC-3 cells were grouped into normal control (NC) and GMF treatment groups. Cell proliferation was analyzed with kit-8 and Ki67 immunofluorescence staining, while cell apoptosis was analyzed with flow cytometry double staining of Annexin V-PE/7-AAD. The Akt and p38 MAPK/Caspase signaling pathways were analyzed by western blotting and immunofluorescence staining, and cell polarization was analyzed with PARD3.

Results: Cell proliferation and activity of the Akt pathway were significantly decreased by the GMF, while cell apoptosis, activity of p38 MAPK, and PARD3-positive cell number were significantly increased in the GMF group compared to the NC group.

Conclusion: GMFs inhibit cell proliferation, induce apoptosis, and regulate tumor cell polarity conditions, potentially through down-regulating Akt, activating the p38 MAPK/Caspase pathway, and promoting PARD3 expression in PC-3 cells.

No MeSH data available.


Related in: MedlinePlus