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Effect of gyromagnetic fields on human prostatic adenocarcinoma cells.

Lei H, Xu Y, Guan R, Li M, Hui Y, Gao Z, Yang B, Xin Z - Onco Targets Ther (2015)

Bottom Line: PC-3 cells were grouped into normal control (NC) and GMF treatment groups.Cell proliferation and activity of the Akt pathway were significantly decreased by the GMF, while cell apoptosis, activity of p38 MAPK, and PARD3-positive cell number were significantly increased in the GMF group compared to the NC group.GMFs inhibit cell proliferation, induce apoptosis, and regulate tumor cell polarity conditions, potentially through down-regulating Akt, activating the p38 MAPK/Caspase pathway, and promoting PARD3 expression in PC-3 cells.

View Article: PubMed Central - PubMed

Affiliation: Andrology Center, Peking University First Hospital, Peking University, Beijing, People's Republic of China.

ABSTRACT

Purpose: To investigate the biological effect of gyromagnetic fields (GMFs) on cell proliferation and apoptosis of human prostatic adenocarcinoma cells and explore the underlying mechanisms.

Methods: PC-3 cells were grouped into normal control (NC) and GMF treatment groups. Cell proliferation was analyzed with kit-8 and Ki67 immunofluorescence staining, while cell apoptosis was analyzed with flow cytometry double staining of Annexin V-PE/7-AAD. The Akt and p38 MAPK/Caspase signaling pathways were analyzed by western blotting and immunofluorescence staining, and cell polarization was analyzed with PARD3.

Results: Cell proliferation and activity of the Akt pathway were significantly decreased by the GMF, while cell apoptosis, activity of p38 MAPK, and PARD3-positive cell number were significantly increased in the GMF group compared to the NC group.

Conclusion: GMFs inhibit cell proliferation, induce apoptosis, and regulate tumor cell polarity conditions, potentially through down-regulating Akt, activating the p38 MAPK/Caspase pathway, and promoting PARD3 expression in PC-3 cells.

No MeSH data available.


Related in: MedlinePlus

Effect of gyromagnetic fields on Akt signaling in PC-3 cells.Notes: (A) Western blot analysis for phospho-Akt and Akt expression in PC-3 cells 24 hours after treatment with a gyromagnetic field from the normal control (NC) group and the gyromagnetic field (GMF) groups with treatment times of 1, 5, 10, and 20 minutes. (B) Quantitative data of band intensity for phospho-Akt and Akt by ImageJ (n=3). *P<0.05 when compared with the NC group.
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f3-ott-8-3489: Effect of gyromagnetic fields on Akt signaling in PC-3 cells.Notes: (A) Western blot analysis for phospho-Akt and Akt expression in PC-3 cells 24 hours after treatment with a gyromagnetic field from the normal control (NC) group and the gyromagnetic field (GMF) groups with treatment times of 1, 5, 10, and 20 minutes. (B) Quantitative data of band intensity for phospho-Akt and Akt by ImageJ (n=3). *P<0.05 when compared with the NC group.

Mentions: To demonstrate the molecular basis of the effects of GMFs on cell proliferation, expression levels of Akt protein, a protein kinase which can promote cell survival, were detected. Twenty-four hours after treatment with a GMF, the phospho-Akt/Akt ratio was significantly decreased in PC-3 cells in the GMF 10 minutes and GMF 20 minutes groups when compared with the NC group (P<0.05; Figure 3), indicating that Akt signaling was inhibited under exposure of the GMF.


Effect of gyromagnetic fields on human prostatic adenocarcinoma cells.

Lei H, Xu Y, Guan R, Li M, Hui Y, Gao Z, Yang B, Xin Z - Onco Targets Ther (2015)

Effect of gyromagnetic fields on Akt signaling in PC-3 cells.Notes: (A) Western blot analysis for phospho-Akt and Akt expression in PC-3 cells 24 hours after treatment with a gyromagnetic field from the normal control (NC) group and the gyromagnetic field (GMF) groups with treatment times of 1, 5, 10, and 20 minutes. (B) Quantitative data of band intensity for phospho-Akt and Akt by ImageJ (n=3). *P<0.05 when compared with the NC group.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4664505&req=5

f3-ott-8-3489: Effect of gyromagnetic fields on Akt signaling in PC-3 cells.Notes: (A) Western blot analysis for phospho-Akt and Akt expression in PC-3 cells 24 hours after treatment with a gyromagnetic field from the normal control (NC) group and the gyromagnetic field (GMF) groups with treatment times of 1, 5, 10, and 20 minutes. (B) Quantitative data of band intensity for phospho-Akt and Akt by ImageJ (n=3). *P<0.05 when compared with the NC group.
Mentions: To demonstrate the molecular basis of the effects of GMFs on cell proliferation, expression levels of Akt protein, a protein kinase which can promote cell survival, were detected. Twenty-four hours after treatment with a GMF, the phospho-Akt/Akt ratio was significantly decreased in PC-3 cells in the GMF 10 minutes and GMF 20 minutes groups when compared with the NC group (P<0.05; Figure 3), indicating that Akt signaling was inhibited under exposure of the GMF.

Bottom Line: PC-3 cells were grouped into normal control (NC) and GMF treatment groups.Cell proliferation and activity of the Akt pathway were significantly decreased by the GMF, while cell apoptosis, activity of p38 MAPK, and PARD3-positive cell number were significantly increased in the GMF group compared to the NC group.GMFs inhibit cell proliferation, induce apoptosis, and regulate tumor cell polarity conditions, potentially through down-regulating Akt, activating the p38 MAPK/Caspase pathway, and promoting PARD3 expression in PC-3 cells.

View Article: PubMed Central - PubMed

Affiliation: Andrology Center, Peking University First Hospital, Peking University, Beijing, People's Republic of China.

ABSTRACT

Purpose: To investigate the biological effect of gyromagnetic fields (GMFs) on cell proliferation and apoptosis of human prostatic adenocarcinoma cells and explore the underlying mechanisms.

Methods: PC-3 cells were grouped into normal control (NC) and GMF treatment groups. Cell proliferation was analyzed with kit-8 and Ki67 immunofluorescence staining, while cell apoptosis was analyzed with flow cytometry double staining of Annexin V-PE/7-AAD. The Akt and p38 MAPK/Caspase signaling pathways were analyzed by western blotting and immunofluorescence staining, and cell polarization was analyzed with PARD3.

Results: Cell proliferation and activity of the Akt pathway were significantly decreased by the GMF, while cell apoptosis, activity of p38 MAPK, and PARD3-positive cell number were significantly increased in the GMF group compared to the NC group.

Conclusion: GMFs inhibit cell proliferation, induce apoptosis, and regulate tumor cell polarity conditions, potentially through down-regulating Akt, activating the p38 MAPK/Caspase pathway, and promoting PARD3 expression in PC-3 cells.

No MeSH data available.


Related in: MedlinePlus