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Effect of gyromagnetic fields on human prostatic adenocarcinoma cells.

Lei H, Xu Y, Guan R, Li M, Hui Y, Gao Z, Yang B, Xin Z - Onco Targets Ther (2015)

Bottom Line: PC-3 cells were grouped into normal control (NC) and GMF treatment groups.Cell proliferation and activity of the Akt pathway were significantly decreased by the GMF, while cell apoptosis, activity of p38 MAPK, and PARD3-positive cell number were significantly increased in the GMF group compared to the NC group.GMFs inhibit cell proliferation, induce apoptosis, and regulate tumor cell polarity conditions, potentially through down-regulating Akt, activating the p38 MAPK/Caspase pathway, and promoting PARD3 expression in PC-3 cells.

View Article: PubMed Central - PubMed

Affiliation: Andrology Center, Peking University First Hospital, Peking University, Beijing, People's Republic of China.

ABSTRACT

Purpose: To investigate the biological effect of gyromagnetic fields (GMFs) on cell proliferation and apoptosis of human prostatic adenocarcinoma cells and explore the underlying mechanisms.

Methods: PC-3 cells were grouped into normal control (NC) and GMF treatment groups. Cell proliferation was analyzed with kit-8 and Ki67 immunofluorescence staining, while cell apoptosis was analyzed with flow cytometry double staining of Annexin V-PE/7-AAD. The Akt and p38 MAPK/Caspase signaling pathways were analyzed by western blotting and immunofluorescence staining, and cell polarization was analyzed with PARD3.

Results: Cell proliferation and activity of the Akt pathway were significantly decreased by the GMF, while cell apoptosis, activity of p38 MAPK, and PARD3-positive cell number were significantly increased in the GMF group compared to the NC group.

Conclusion: GMFs inhibit cell proliferation, induce apoptosis, and regulate tumor cell polarity conditions, potentially through down-regulating Akt, activating the p38 MAPK/Caspase pathway, and promoting PARD3 expression in PC-3 cells.

No MeSH data available.


Related in: MedlinePlus

Effect of gyromagnetic fields on cell proliferation in PC-3 cells.Notes: (A) Quantitative analysis of cell number counting assayed with CCK-8 in PC-3 cells after 24 hours treatment with a gyromagnetic field from the normal control (NC) group and the gyromagnetic field (GMF) groups with treatment times of 1, 5, 10, and 20 minutes (n=3). (B) Cell numbers were checked 24 hours after each treatment with a gyromagnetic field in PC-3 cells from the NC group and cells treated with a magnetic field for 10 minutes each time (GMF 10 minutes, once a day); (n=3). (C) Ki67 (red) immunofluorescence staining in PC-3 cells 24 hours after treatment with a gyromagnetic field from the NC group and cells treated once with a magnetic field for 10 minutes (GMF 10 minutes). Nuclei were labeled with 4′,6-diamidino-2-phenylindole (DAPI) (blue). Scale bars=50 μm, 200×. (D) Quantitative data of Ki67-positive cell number per high power field (HPF) by Image-Pro plus (n=3). *P<0.05 when compared with the NC group.
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f2-ott-8-3489: Effect of gyromagnetic fields on cell proliferation in PC-3 cells.Notes: (A) Quantitative analysis of cell number counting assayed with CCK-8 in PC-3 cells after 24 hours treatment with a gyromagnetic field from the normal control (NC) group and the gyromagnetic field (GMF) groups with treatment times of 1, 5, 10, and 20 minutes (n=3). (B) Cell numbers were checked 24 hours after each treatment with a gyromagnetic field in PC-3 cells from the NC group and cells treated with a magnetic field for 10 minutes each time (GMF 10 minutes, once a day); (n=3). (C) Ki67 (red) immunofluorescence staining in PC-3 cells 24 hours after treatment with a gyromagnetic field from the NC group and cells treated once with a magnetic field for 10 minutes (GMF 10 minutes). Nuclei were labeled with 4′,6-diamidino-2-phenylindole (DAPI) (blue). Scale bars=50 μm, 200×. (D) Quantitative data of Ki67-positive cell number per high power field (HPF) by Image-Pro plus (n=3). *P<0.05 when compared with the NC group.

Mentions: To investigate the effects of GMFs on cell proliferation in PC-3 cells, a cell-counting assay was performed 24 hours after GMF treatment. Cell proliferation was significantly inhibited when compared with the normal control (NC) group (P<0.05), especially in the GMF 10-minute group (Figure 2A). We then checked cell numbers in the GMF 10 minutes versus the NC group for 4 days; cell growth was significantly inhibited in the GMF 10-minute group (P<0.05; Figure 2B).


Effect of gyromagnetic fields on human prostatic adenocarcinoma cells.

Lei H, Xu Y, Guan R, Li M, Hui Y, Gao Z, Yang B, Xin Z - Onco Targets Ther (2015)

Effect of gyromagnetic fields on cell proliferation in PC-3 cells.Notes: (A) Quantitative analysis of cell number counting assayed with CCK-8 in PC-3 cells after 24 hours treatment with a gyromagnetic field from the normal control (NC) group and the gyromagnetic field (GMF) groups with treatment times of 1, 5, 10, and 20 minutes (n=3). (B) Cell numbers were checked 24 hours after each treatment with a gyromagnetic field in PC-3 cells from the NC group and cells treated with a magnetic field for 10 minutes each time (GMF 10 minutes, once a day); (n=3). (C) Ki67 (red) immunofluorescence staining in PC-3 cells 24 hours after treatment with a gyromagnetic field from the NC group and cells treated once with a magnetic field for 10 minutes (GMF 10 minutes). Nuclei were labeled with 4′,6-diamidino-2-phenylindole (DAPI) (blue). Scale bars=50 μm, 200×. (D) Quantitative data of Ki67-positive cell number per high power field (HPF) by Image-Pro plus (n=3). *P<0.05 when compared with the NC group.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4664505&req=5

f2-ott-8-3489: Effect of gyromagnetic fields on cell proliferation in PC-3 cells.Notes: (A) Quantitative analysis of cell number counting assayed with CCK-8 in PC-3 cells after 24 hours treatment with a gyromagnetic field from the normal control (NC) group and the gyromagnetic field (GMF) groups with treatment times of 1, 5, 10, and 20 minutes (n=3). (B) Cell numbers were checked 24 hours after each treatment with a gyromagnetic field in PC-3 cells from the NC group and cells treated with a magnetic field for 10 minutes each time (GMF 10 minutes, once a day); (n=3). (C) Ki67 (red) immunofluorescence staining in PC-3 cells 24 hours after treatment with a gyromagnetic field from the NC group and cells treated once with a magnetic field for 10 minutes (GMF 10 minutes). Nuclei were labeled with 4′,6-diamidino-2-phenylindole (DAPI) (blue). Scale bars=50 μm, 200×. (D) Quantitative data of Ki67-positive cell number per high power field (HPF) by Image-Pro plus (n=3). *P<0.05 when compared with the NC group.
Mentions: To investigate the effects of GMFs on cell proliferation in PC-3 cells, a cell-counting assay was performed 24 hours after GMF treatment. Cell proliferation was significantly inhibited when compared with the normal control (NC) group (P<0.05), especially in the GMF 10-minute group (Figure 2A). We then checked cell numbers in the GMF 10 minutes versus the NC group for 4 days; cell growth was significantly inhibited in the GMF 10-minute group (P<0.05; Figure 2B).

Bottom Line: PC-3 cells were grouped into normal control (NC) and GMF treatment groups.Cell proliferation and activity of the Akt pathway were significantly decreased by the GMF, while cell apoptosis, activity of p38 MAPK, and PARD3-positive cell number were significantly increased in the GMF group compared to the NC group.GMFs inhibit cell proliferation, induce apoptosis, and regulate tumor cell polarity conditions, potentially through down-regulating Akt, activating the p38 MAPK/Caspase pathway, and promoting PARD3 expression in PC-3 cells.

View Article: PubMed Central - PubMed

Affiliation: Andrology Center, Peking University First Hospital, Peking University, Beijing, People's Republic of China.

ABSTRACT

Purpose: To investigate the biological effect of gyromagnetic fields (GMFs) on cell proliferation and apoptosis of human prostatic adenocarcinoma cells and explore the underlying mechanisms.

Methods: PC-3 cells were grouped into normal control (NC) and GMF treatment groups. Cell proliferation was analyzed with kit-8 and Ki67 immunofluorescence staining, while cell apoptosis was analyzed with flow cytometry double staining of Annexin V-PE/7-AAD. The Akt and p38 MAPK/Caspase signaling pathways were analyzed by western blotting and immunofluorescence staining, and cell polarization was analyzed with PARD3.

Results: Cell proliferation and activity of the Akt pathway were significantly decreased by the GMF, while cell apoptosis, activity of p38 MAPK, and PARD3-positive cell number were significantly increased in the GMF group compared to the NC group.

Conclusion: GMFs inhibit cell proliferation, induce apoptosis, and regulate tumor cell polarity conditions, potentially through down-regulating Akt, activating the p38 MAPK/Caspase pathway, and promoting PARD3 expression in PC-3 cells.

No MeSH data available.


Related in: MedlinePlus