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Effect of gyromagnetic fields on human prostatic adenocarcinoma cells.

Lei H, Xu Y, Guan R, Li M, Hui Y, Gao Z, Yang B, Xin Z - Onco Targets Ther (2015)

Bottom Line: PC-3 cells were grouped into normal control (NC) and GMF treatment groups.Cell proliferation and activity of the Akt pathway were significantly decreased by the GMF, while cell apoptosis, activity of p38 MAPK, and PARD3-positive cell number were significantly increased in the GMF group compared to the NC group.GMFs inhibit cell proliferation, induce apoptosis, and regulate tumor cell polarity conditions, potentially through down-regulating Akt, activating the p38 MAPK/Caspase pathway, and promoting PARD3 expression in PC-3 cells.

View Article: PubMed Central - PubMed

Affiliation: Andrology Center, Peking University First Hospital, Peking University, Beijing, People's Republic of China.

ABSTRACT

Purpose: To investigate the biological effect of gyromagnetic fields (GMFs) on cell proliferation and apoptosis of human prostatic adenocarcinoma cells and explore the underlying mechanisms.

Methods: PC-3 cells were grouped into normal control (NC) and GMF treatment groups. Cell proliferation was analyzed with kit-8 and Ki67 immunofluorescence staining, while cell apoptosis was analyzed with flow cytometry double staining of Annexin V-PE/7-AAD. The Akt and p38 MAPK/Caspase signaling pathways were analyzed by western blotting and immunofluorescence staining, and cell polarization was analyzed with PARD3.

Results: Cell proliferation and activity of the Akt pathway were significantly decreased by the GMF, while cell apoptosis, activity of p38 MAPK, and PARD3-positive cell number were significantly increased in the GMF group compared to the NC group.

Conclusion: GMFs inhibit cell proliferation, induce apoptosis, and regulate tumor cell polarity conditions, potentially through down-regulating Akt, activating the p38 MAPK/Caspase pathway, and promoting PARD3 expression in PC-3 cells.

No MeSH data available.


Related in: MedlinePlus

Gyromagnetic field strength distribution varying with time using a CKJ-II (QLX-II) Gyromagnetic Therapy Machine.Notes: Gyromagnetic field strength varied with time in the target area and obeyed the distribution regularity of a sine curve. The rotating frequency of the gyromagnetic field was 6 Hz, the maximum field strength was 300 mT, and the magnetic flux density was 8.3 mT/Hz2.
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f1-ott-8-3489: Gyromagnetic field strength distribution varying with time using a CKJ-II (QLX-II) Gyromagnetic Therapy Machine.Notes: Gyromagnetic field strength varied with time in the target area and obeyed the distribution regularity of a sine curve. The rotating frequency of the gyromagnetic field was 6 Hz, the maximum field strength was 300 mT, and the magnetic flux density was 8.3 mT/Hz2.

Mentions: PC-3 experimental groups were exposed to GMFs by a CKJ-II (QLX-II) Gyromagnetic Therapy Machine (Beijing Gyromagnetic Medical Equipment Co., Ltd., Beijing, People’s Republic of China). The work parameters were the GMF strength varied with time in the target area and obeyed the distribution regularity of a sine curve; the rotating frequency of the GMF was 6 Hz; the maximum field strength was 300 mT; and the magnetic flux density was 8.3 mT/Hz2 (Figure 1). The top and bottom surfaces of the two magnetic poles were at a distance of 32 cm. Cell samples were placed in the gyromagnetic therapeutic machine according to the manufacturer’s instructions.


Effect of gyromagnetic fields on human prostatic adenocarcinoma cells.

Lei H, Xu Y, Guan R, Li M, Hui Y, Gao Z, Yang B, Xin Z - Onco Targets Ther (2015)

Gyromagnetic field strength distribution varying with time using a CKJ-II (QLX-II) Gyromagnetic Therapy Machine.Notes: Gyromagnetic field strength varied with time in the target area and obeyed the distribution regularity of a sine curve. The rotating frequency of the gyromagnetic field was 6 Hz, the maximum field strength was 300 mT, and the magnetic flux density was 8.3 mT/Hz2.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4664505&req=5

f1-ott-8-3489: Gyromagnetic field strength distribution varying with time using a CKJ-II (QLX-II) Gyromagnetic Therapy Machine.Notes: Gyromagnetic field strength varied with time in the target area and obeyed the distribution regularity of a sine curve. The rotating frequency of the gyromagnetic field was 6 Hz, the maximum field strength was 300 mT, and the magnetic flux density was 8.3 mT/Hz2.
Mentions: PC-3 experimental groups were exposed to GMFs by a CKJ-II (QLX-II) Gyromagnetic Therapy Machine (Beijing Gyromagnetic Medical Equipment Co., Ltd., Beijing, People’s Republic of China). The work parameters were the GMF strength varied with time in the target area and obeyed the distribution regularity of a sine curve; the rotating frequency of the GMF was 6 Hz; the maximum field strength was 300 mT; and the magnetic flux density was 8.3 mT/Hz2 (Figure 1). The top and bottom surfaces of the two magnetic poles were at a distance of 32 cm. Cell samples were placed in the gyromagnetic therapeutic machine according to the manufacturer’s instructions.

Bottom Line: PC-3 cells were grouped into normal control (NC) and GMF treatment groups.Cell proliferation and activity of the Akt pathway were significantly decreased by the GMF, while cell apoptosis, activity of p38 MAPK, and PARD3-positive cell number were significantly increased in the GMF group compared to the NC group.GMFs inhibit cell proliferation, induce apoptosis, and regulate tumor cell polarity conditions, potentially through down-regulating Akt, activating the p38 MAPK/Caspase pathway, and promoting PARD3 expression in PC-3 cells.

View Article: PubMed Central - PubMed

Affiliation: Andrology Center, Peking University First Hospital, Peking University, Beijing, People's Republic of China.

ABSTRACT

Purpose: To investigate the biological effect of gyromagnetic fields (GMFs) on cell proliferation and apoptosis of human prostatic adenocarcinoma cells and explore the underlying mechanisms.

Methods: PC-3 cells were grouped into normal control (NC) and GMF treatment groups. Cell proliferation was analyzed with kit-8 and Ki67 immunofluorescence staining, while cell apoptosis was analyzed with flow cytometry double staining of Annexin V-PE/7-AAD. The Akt and p38 MAPK/Caspase signaling pathways were analyzed by western blotting and immunofluorescence staining, and cell polarization was analyzed with PARD3.

Results: Cell proliferation and activity of the Akt pathway were significantly decreased by the GMF, while cell apoptosis, activity of p38 MAPK, and PARD3-positive cell number were significantly increased in the GMF group compared to the NC group.

Conclusion: GMFs inhibit cell proliferation, induce apoptosis, and regulate tumor cell polarity conditions, potentially through down-regulating Akt, activating the p38 MAPK/Caspase pathway, and promoting PARD3 expression in PC-3 cells.

No MeSH data available.


Related in: MedlinePlus