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The antiplasmodial and spleen protective role of crude Indigofera oblongifolia leaf extract traditionally used in the treatment of malaria in Saudi Arabia.

Dkhil MA, Lubbad MY, Al-Shaebi EM, Delic D, Al-Quraishy S - Drug Des Devel Ther (2015)

Bottom Line: The suppressive effect produced by the 100 mg/kg dose on parasitemia was highly significant compared to the infected nontreated group.Among the differentially up- and downregulated genes under the effect of IOLE, we quantified the expression of Ccl8, Saa3, Cd209a, and Cd209b mRNAs.Based on our results, I. oblongifolia exhibits antimalarial activity and could protect the spleen from P. chabaudi-induced injury.

View Article: PubMed Central - PubMed

Affiliation: Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia ; Department of Zoology and Entomology, Faculty of Science, Helwan University, Helwan, Egypt.

ABSTRACT
Malaria is one of the most serious natural hazards faced by human society. Although plant leaves of Indigofera oblongifolia have been used for the treatment of malaria in Saudi Arabian society, there is no laboratory-based evidence for the effectiveness and safety of the plant. This study therefore was designed to investigate the antimalarial and spleen protective activity of I. oblongifolia leaf extract (IOLE) in mice. Three doses (100, 200 and 300 mg/kg) of IOLE were used to treat mice infected with Plasmodium chabaudi-parasitized erythrocytes. The suppressive effect produced by the 100 mg/kg dose on parasitemia was highly significant compared to the infected nontreated group. This dose was also able to repair the change in the thickness of the mice spleen and significantly lower the number of apoptotic cells in the spleen. Moreover, I. oblongifolia also altered gene expression in the infected spleen. On day 7 postinfection, the mRNA expression of six genes - with immune response functions - was upregulated by more than twofold, while that of 24 other genes was downregulated. Among the differentially up- and downregulated genes under the effect of IOLE, we quantified the expression of Ccl8, Saa3, Cd209a, and Cd209b mRNAs. The expression data, determined by microarrays, were largely consistent with the expression analyses we performed with several arbitrarily selected genes using quantitative polymerase chain reaction (PCR). Based on our results, I. oblongifolia exhibits antimalarial activity and could protect the spleen from P. chabaudi-induced injury.

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Related in: MedlinePlus

Indigofera oblongifolia-induced apoptotic changes in mouse spleens infected with Plasmodiun chabaudi at day 7 pi.Notes: (A) Uninfected spleen. (B) Infected spleen. (C) Infected I. oblongifolia treated (100 mg/kg) spleen. (D) Infected I. oblongifolia treated (200 mg/kg) spleen. (E) Infected I. oblongifolia treated (300 mg/kg) spleen. TUNEL-positive cells appeared brown. Scale bar =25 μm.Abbreviations: TUNEL, terminal deoxynucleotidyl-transferase-mediated dUTP nick end labeling assay; pi, postinfection.
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f4-dddt-9-6235: Indigofera oblongifolia-induced apoptotic changes in mouse spleens infected with Plasmodiun chabaudi at day 7 pi.Notes: (A) Uninfected spleen. (B) Infected spleen. (C) Infected I. oblongifolia treated (100 mg/kg) spleen. (D) Infected I. oblongifolia treated (200 mg/kg) spleen. (E) Infected I. oblongifolia treated (300 mg/kg) spleen. TUNEL-positive cells appeared brown. Scale bar =25 μm.Abbreviations: TUNEL, terminal deoxynucleotidyl-transferase-mediated dUTP nick end labeling assay; pi, postinfection.

Mentions: A higher number of TUNEL-positive cells were shown in spleen sections of mice infected with P. chabaudi-parasitized erythrocytes on day 7 pi (Figures 4 and 5). I. oblongifolia was able to reduce the number of TUNEL-positive cells in the spleen of mice treated with a dose of 100 mg IOLE/kg (Figures 4 and 5). However, the spleen of mice treated either with 200 or 300 mg IOLE/kg showed no significant difference in the number of TUNEL-positive cells compared to the infected group (Figure 5).


The antiplasmodial and spleen protective role of crude Indigofera oblongifolia leaf extract traditionally used in the treatment of malaria in Saudi Arabia.

Dkhil MA, Lubbad MY, Al-Shaebi EM, Delic D, Al-Quraishy S - Drug Des Devel Ther (2015)

Indigofera oblongifolia-induced apoptotic changes in mouse spleens infected with Plasmodiun chabaudi at day 7 pi.Notes: (A) Uninfected spleen. (B) Infected spleen. (C) Infected I. oblongifolia treated (100 mg/kg) spleen. (D) Infected I. oblongifolia treated (200 mg/kg) spleen. (E) Infected I. oblongifolia treated (300 mg/kg) spleen. TUNEL-positive cells appeared brown. Scale bar =25 μm.Abbreviations: TUNEL, terminal deoxynucleotidyl-transferase-mediated dUTP nick end labeling assay; pi, postinfection.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4664501&req=5

f4-dddt-9-6235: Indigofera oblongifolia-induced apoptotic changes in mouse spleens infected with Plasmodiun chabaudi at day 7 pi.Notes: (A) Uninfected spleen. (B) Infected spleen. (C) Infected I. oblongifolia treated (100 mg/kg) spleen. (D) Infected I. oblongifolia treated (200 mg/kg) spleen. (E) Infected I. oblongifolia treated (300 mg/kg) spleen. TUNEL-positive cells appeared brown. Scale bar =25 μm.Abbreviations: TUNEL, terminal deoxynucleotidyl-transferase-mediated dUTP nick end labeling assay; pi, postinfection.
Mentions: A higher number of TUNEL-positive cells were shown in spleen sections of mice infected with P. chabaudi-parasitized erythrocytes on day 7 pi (Figures 4 and 5). I. oblongifolia was able to reduce the number of TUNEL-positive cells in the spleen of mice treated with a dose of 100 mg IOLE/kg (Figures 4 and 5). However, the spleen of mice treated either with 200 or 300 mg IOLE/kg showed no significant difference in the number of TUNEL-positive cells compared to the infected group (Figure 5).

Bottom Line: The suppressive effect produced by the 100 mg/kg dose on parasitemia was highly significant compared to the infected nontreated group.Among the differentially up- and downregulated genes under the effect of IOLE, we quantified the expression of Ccl8, Saa3, Cd209a, and Cd209b mRNAs.Based on our results, I. oblongifolia exhibits antimalarial activity and could protect the spleen from P. chabaudi-induced injury.

View Article: PubMed Central - PubMed

Affiliation: Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia ; Department of Zoology and Entomology, Faculty of Science, Helwan University, Helwan, Egypt.

ABSTRACT
Malaria is one of the most serious natural hazards faced by human society. Although plant leaves of Indigofera oblongifolia have been used for the treatment of malaria in Saudi Arabian society, there is no laboratory-based evidence for the effectiveness and safety of the plant. This study therefore was designed to investigate the antimalarial and spleen protective activity of I. oblongifolia leaf extract (IOLE) in mice. Three doses (100, 200 and 300 mg/kg) of IOLE were used to treat mice infected with Plasmodium chabaudi-parasitized erythrocytes. The suppressive effect produced by the 100 mg/kg dose on parasitemia was highly significant compared to the infected nontreated group. This dose was also able to repair the change in the thickness of the mice spleen and significantly lower the number of apoptotic cells in the spleen. Moreover, I. oblongifolia also altered gene expression in the infected spleen. On day 7 postinfection, the mRNA expression of six genes - with immune response functions - was upregulated by more than twofold, while that of 24 other genes was downregulated. Among the differentially up- and downregulated genes under the effect of IOLE, we quantified the expression of Ccl8, Saa3, Cd209a, and Cd209b mRNAs. The expression data, determined by microarrays, were largely consistent with the expression analyses we performed with several arbitrarily selected genes using quantitative polymerase chain reaction (PCR). Based on our results, I. oblongifolia exhibits antimalarial activity and could protect the spleen from P. chabaudi-induced injury.

Show MeSH
Related in: MedlinePlus