Limits...
The diameter of nanotubes formed on Ti-6Al-4V alloy controls the adhesion and differentiation of Saos-2 cells.

Filova E, Fojt J, Kryslova M, Moravec H, Joska L, Bacakova L - Int J Nanomedicine (2015)

Bottom Line: On day 3, the highest concentrations of both vinculin and talin measured by enzyme-linked immunosorbent assay and intensity of immunofluorescence staining were on 30 V nanotubes.On the other hand, the highest concentrations of ALP, type I collagen, and osteopontin were found on 10 V and 20 V samples.Therefore, the controlled anodization of Ti-6Al-4V seems to be a useful tool for preparing nanostructured materials with desirable biological properties.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomaterials and Tissue Engineering, Institute of Physiology, Czech Academy of Sciences, Prague, Czech Republic.

ABSTRACT
Ti-6Al-4V-based nanotubes were prepared on a Ti-6Al-4V surface by anodic oxidation on 10 V, 20 V, and 30 V samples. The 10 V, 20 V, and 30 V samples and a control smooth Ti-6Al-4V sample were evaluated in terms of their chemical composition, diameter distribution, and cellular response. The surfaces of the 10 V, 20 V, and 30 V samples consisted of nanotubes of a relatively wide range of diameters that increased with the voltage. Saos-2 cells had a similar initial adhesion on all nanotube samples to the control Ti-6Al-4V sample, but it was lower than on glass. On day 3, the highest concentrations of both vinculin and talin measured by enzyme-linked immunosorbent assay and intensity of immunofluorescence staining were on 30 V nanotubes. On the other hand, the highest concentrations of ALP, type I collagen, and osteopontin were found on 10 V and 20 V samples. The final cellular densities on 10 V, 20 V, and 30 V samples were higher than on glass. Therefore, the controlled anodization of Ti-6Al-4V seems to be a useful tool for preparing nanostructured materials with desirable biological properties.

No MeSH data available.


Related in: MedlinePlus

Human Saos-2 osteoblasts on 10 V, 20 V, and 30 V nanotubes, on control Ti_C, and on glass coverslips.Notes: Absorbance of collagen type I (A), ALP (B), osteopontin (C), osteocalcin (D), and calcium content (E). Measured by enzyme-linked immunosorbent assay (A–D) and by Alizarin staining (E) on day 7. Data expressed as mean ± standard error of mean. P≤0.05 considered significant in comparison with samples labeled above columns.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4664495&req=5

f9-ijn-10-7145: Human Saos-2 osteoblasts on 10 V, 20 V, and 30 V nanotubes, on control Ti_C, and on glass coverslips.Notes: Absorbance of collagen type I (A), ALP (B), osteopontin (C), osteocalcin (D), and calcium content (E). Measured by enzyme-linked immunosorbent assay (A–D) and by Alizarin staining (E) on day 7. Data expressed as mean ± standard error of mean. P≤0.05 considered significant in comparison with samples labeled above columns.

Mentions: ELISA revealed the highest concentration of collagen I on 10 V, 20 V, and 30 V nanotubes, and this value significantly decreased on Ti_C and glass (Figure 9A). On the nanotubes, the average concentration tended to decrease from 10 V to 30 V nanotubes, but these differences were not significant. The decrease was more pronounced in the concentration of ALP, which was higher in cells on 10 V and 20 V nanotubes in comparison with cells on 30 V nanotubes and glass (Figure 9B). The middle and late markers of osteogenic cell differentiation showed an even clearer tendency to decrease with increasing nanotube diameter. The concentration of osteopontin decreased gradually from maximum values on 10 V nanotubes to lower values on 20 V and 30 V nanotubes and Ti_C to the minimum value on glass (Figure 9C). Also, the concentration of osteocalcin in cells on 30 V nanotubes was significantly lower than on 20 V nanotubes, and very low on glass and Ti_C (Figure 9D). The quantification of calcium by Alizarin Red staining (Figure 9E) showed an increased concentration of calcium on the Ti_C sample compared to glass, while the calcium concentration in cells on all nanotube samples was similar to the value on glass.


The diameter of nanotubes formed on Ti-6Al-4V alloy controls the adhesion and differentiation of Saos-2 cells.

Filova E, Fojt J, Kryslova M, Moravec H, Joska L, Bacakova L - Int J Nanomedicine (2015)

Human Saos-2 osteoblasts on 10 V, 20 V, and 30 V nanotubes, on control Ti_C, and on glass coverslips.Notes: Absorbance of collagen type I (A), ALP (B), osteopontin (C), osteocalcin (D), and calcium content (E). Measured by enzyme-linked immunosorbent assay (A–D) and by Alizarin staining (E) on day 7. Data expressed as mean ± standard error of mean. P≤0.05 considered significant in comparison with samples labeled above columns.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4664495&req=5

f9-ijn-10-7145: Human Saos-2 osteoblasts on 10 V, 20 V, and 30 V nanotubes, on control Ti_C, and on glass coverslips.Notes: Absorbance of collagen type I (A), ALP (B), osteopontin (C), osteocalcin (D), and calcium content (E). Measured by enzyme-linked immunosorbent assay (A–D) and by Alizarin staining (E) on day 7. Data expressed as mean ± standard error of mean. P≤0.05 considered significant in comparison with samples labeled above columns.
Mentions: ELISA revealed the highest concentration of collagen I on 10 V, 20 V, and 30 V nanotubes, and this value significantly decreased on Ti_C and glass (Figure 9A). On the nanotubes, the average concentration tended to decrease from 10 V to 30 V nanotubes, but these differences were not significant. The decrease was more pronounced in the concentration of ALP, which was higher in cells on 10 V and 20 V nanotubes in comparison with cells on 30 V nanotubes and glass (Figure 9B). The middle and late markers of osteogenic cell differentiation showed an even clearer tendency to decrease with increasing nanotube diameter. The concentration of osteopontin decreased gradually from maximum values on 10 V nanotubes to lower values on 20 V and 30 V nanotubes and Ti_C to the minimum value on glass (Figure 9C). Also, the concentration of osteocalcin in cells on 30 V nanotubes was significantly lower than on 20 V nanotubes, and very low on glass and Ti_C (Figure 9D). The quantification of calcium by Alizarin Red staining (Figure 9E) showed an increased concentration of calcium on the Ti_C sample compared to glass, while the calcium concentration in cells on all nanotube samples was similar to the value on glass.

Bottom Line: On day 3, the highest concentrations of both vinculin and talin measured by enzyme-linked immunosorbent assay and intensity of immunofluorescence staining were on 30 V nanotubes.On the other hand, the highest concentrations of ALP, type I collagen, and osteopontin were found on 10 V and 20 V samples.Therefore, the controlled anodization of Ti-6Al-4V seems to be a useful tool for preparing nanostructured materials with desirable biological properties.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomaterials and Tissue Engineering, Institute of Physiology, Czech Academy of Sciences, Prague, Czech Republic.

ABSTRACT
Ti-6Al-4V-based nanotubes were prepared on a Ti-6Al-4V surface by anodic oxidation on 10 V, 20 V, and 30 V samples. The 10 V, 20 V, and 30 V samples and a control smooth Ti-6Al-4V sample were evaluated in terms of their chemical composition, diameter distribution, and cellular response. The surfaces of the 10 V, 20 V, and 30 V samples consisted of nanotubes of a relatively wide range of diameters that increased with the voltage. Saos-2 cells had a similar initial adhesion on all nanotube samples to the control Ti-6Al-4V sample, but it was lower than on glass. On day 3, the highest concentrations of both vinculin and talin measured by enzyme-linked immunosorbent assay and intensity of immunofluorescence staining were on 30 V nanotubes. On the other hand, the highest concentrations of ALP, type I collagen, and osteopontin were found on 10 V and 20 V samples. The final cellular densities on 10 V, 20 V, and 30 V samples were higher than on glass. Therefore, the controlled anodization of Ti-6Al-4V seems to be a useful tool for preparing nanostructured materials with desirable biological properties.

No MeSH data available.


Related in: MedlinePlus