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The diameter of nanotubes formed on Ti-6Al-4V alloy controls the adhesion and differentiation of Saos-2 cells.

Filova E, Fojt J, Kryslova M, Moravec H, Joska L, Bacakova L - Int J Nanomedicine (2015)

Bottom Line: On day 3, the highest concentrations of both vinculin and talin measured by enzyme-linked immunosorbent assay and intensity of immunofluorescence staining were on 30 V nanotubes.On the other hand, the highest concentrations of ALP, type I collagen, and osteopontin were found on 10 V and 20 V samples.Therefore, the controlled anodization of Ti-6Al-4V seems to be a useful tool for preparing nanostructured materials with desirable biological properties.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomaterials and Tissue Engineering, Institute of Physiology, Czech Academy of Sciences, Prague, Czech Republic.

ABSTRACT
Ti-6Al-4V-based nanotubes were prepared on a Ti-6Al-4V surface by anodic oxidation on 10 V, 20 V, and 30 V samples. The 10 V, 20 V, and 30 V samples and a control smooth Ti-6Al-4V sample were evaluated in terms of their chemical composition, diameter distribution, and cellular response. The surfaces of the 10 V, 20 V, and 30 V samples consisted of nanotubes of a relatively wide range of diameters that increased with the voltage. Saos-2 cells had a similar initial adhesion on all nanotube samples to the control Ti-6Al-4V sample, but it was lower than on glass. On day 3, the highest concentrations of both vinculin and talin measured by enzyme-linked immunosorbent assay and intensity of immunofluorescence staining were on 30 V nanotubes. On the other hand, the highest concentrations of ALP, type I collagen, and osteopontin were found on 10 V and 20 V samples. The final cellular densities on 10 V, 20 V, and 30 V samples were higher than on glass. Therefore, the controlled anodization of Ti-6Al-4V seems to be a useful tool for preparing nanostructured materials with desirable biological properties.

No MeSH data available.


Related in: MedlinePlus

Human Saos-2 osteoblasts on 10 V, 20 V, and 30 V nanotubes, on control Ti_C, and on glass coverslips on day 7.Notes: Immunofluorescence intensity of collagen (A), ALP (B), osteopontin (C), and osteocalcin (D). Data expressed as means ± standard error of mean. P≤0.05 considered significant in comparison with samples labeled above columns.
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f8-ijn-10-7145: Human Saos-2 osteoblasts on 10 V, 20 V, and 30 V nanotubes, on control Ti_C, and on glass coverslips on day 7.Notes: Immunofluorescence intensity of collagen (A), ALP (B), osteopontin (C), and osteocalcin (D). Data expressed as means ± standard error of mean. P≤0.05 considered significant in comparison with samples labeled above columns.

Mentions: Staining of type I collagen showed the highest signal per cell on the 20 V samples and the lowest on the 30 V sample (Figures 8A and S2). Relatively high fluorescence intensity was also found on Ti_C. The fluorescence signal of ALP was high in cells on 10 V and 20 V samples and decreased on the largest nanotubes, ie, on 30 V; the lowest intensity was observed on glass and Ti_C (Figures 8B and S3). On the other hand, after osteopontin staining, signal intensity increased with nanotube diameter, but it was relatively high on Ti_C too (Figures 8C and S4). Osteocalcin, a late osteogenic marker, was most intensively stained on 20 V and 30 V nanotubes and on Ti_C, and less intensively on the glass and 10 V sample (Figures 8D and S5). Therefore, it can be summarized that the concentration of markers of early osteogenic differentiation showed a tendency to decrease with increasing nanotube diameter, while the middle and late markers rather increased.


The diameter of nanotubes formed on Ti-6Al-4V alloy controls the adhesion and differentiation of Saos-2 cells.

Filova E, Fojt J, Kryslova M, Moravec H, Joska L, Bacakova L - Int J Nanomedicine (2015)

Human Saos-2 osteoblasts on 10 V, 20 V, and 30 V nanotubes, on control Ti_C, and on glass coverslips on day 7.Notes: Immunofluorescence intensity of collagen (A), ALP (B), osteopontin (C), and osteocalcin (D). Data expressed as means ± standard error of mean. P≤0.05 considered significant in comparison with samples labeled above columns.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4664495&req=5

f8-ijn-10-7145: Human Saos-2 osteoblasts on 10 V, 20 V, and 30 V nanotubes, on control Ti_C, and on glass coverslips on day 7.Notes: Immunofluorescence intensity of collagen (A), ALP (B), osteopontin (C), and osteocalcin (D). Data expressed as means ± standard error of mean. P≤0.05 considered significant in comparison with samples labeled above columns.
Mentions: Staining of type I collagen showed the highest signal per cell on the 20 V samples and the lowest on the 30 V sample (Figures 8A and S2). Relatively high fluorescence intensity was also found on Ti_C. The fluorescence signal of ALP was high in cells on 10 V and 20 V samples and decreased on the largest nanotubes, ie, on 30 V; the lowest intensity was observed on glass and Ti_C (Figures 8B and S3). On the other hand, after osteopontin staining, signal intensity increased with nanotube diameter, but it was relatively high on Ti_C too (Figures 8C and S4). Osteocalcin, a late osteogenic marker, was most intensively stained on 20 V and 30 V nanotubes and on Ti_C, and less intensively on the glass and 10 V sample (Figures 8D and S5). Therefore, it can be summarized that the concentration of markers of early osteogenic differentiation showed a tendency to decrease with increasing nanotube diameter, while the middle and late markers rather increased.

Bottom Line: On day 3, the highest concentrations of both vinculin and talin measured by enzyme-linked immunosorbent assay and intensity of immunofluorescence staining were on 30 V nanotubes.On the other hand, the highest concentrations of ALP, type I collagen, and osteopontin were found on 10 V and 20 V samples.Therefore, the controlled anodization of Ti-6Al-4V seems to be a useful tool for preparing nanostructured materials with desirable biological properties.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomaterials and Tissue Engineering, Institute of Physiology, Czech Academy of Sciences, Prague, Czech Republic.

ABSTRACT
Ti-6Al-4V-based nanotubes were prepared on a Ti-6Al-4V surface by anodic oxidation on 10 V, 20 V, and 30 V samples. The 10 V, 20 V, and 30 V samples and a control smooth Ti-6Al-4V sample were evaluated in terms of their chemical composition, diameter distribution, and cellular response. The surfaces of the 10 V, 20 V, and 30 V samples consisted of nanotubes of a relatively wide range of diameters that increased with the voltage. Saos-2 cells had a similar initial adhesion on all nanotube samples to the control Ti-6Al-4V sample, but it was lower than on glass. On day 3, the highest concentrations of both vinculin and talin measured by enzyme-linked immunosorbent assay and intensity of immunofluorescence staining were on 30 V nanotubes. On the other hand, the highest concentrations of ALP, type I collagen, and osteopontin were found on 10 V and 20 V samples. The final cellular densities on 10 V, 20 V, and 30 V samples were higher than on glass. Therefore, the controlled anodization of Ti-6Al-4V seems to be a useful tool for preparing nanostructured materials with desirable biological properties.

No MeSH data available.


Related in: MedlinePlus