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The diameter of nanotubes formed on Ti-6Al-4V alloy controls the adhesion and differentiation of Saos-2 cells.

Filova E, Fojt J, Kryslova M, Moravec H, Joska L, Bacakova L - Int J Nanomedicine (2015)

Bottom Line: On day 3, the highest concentrations of both vinculin and talin measured by enzyme-linked immunosorbent assay and intensity of immunofluorescence staining were on 30 V nanotubes.On the other hand, the highest concentrations of ALP, type I collagen, and osteopontin were found on 10 V and 20 V samples.Therefore, the controlled anodization of Ti-6Al-4V seems to be a useful tool for preparing nanostructured materials with desirable biological properties.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomaterials and Tissue Engineering, Institute of Physiology, Czech Academy of Sciences, Prague, Czech Republic.

ABSTRACT
Ti-6Al-4V-based nanotubes were prepared on a Ti-6Al-4V surface by anodic oxidation on 10 V, 20 V, and 30 V samples. The 10 V, 20 V, and 30 V samples and a control smooth Ti-6Al-4V sample were evaluated in terms of their chemical composition, diameter distribution, and cellular response. The surfaces of the 10 V, 20 V, and 30 V samples consisted of nanotubes of a relatively wide range of diameters that increased with the voltage. Saos-2 cells had a similar initial adhesion on all nanotube samples to the control Ti-6Al-4V sample, but it was lower than on glass. On day 3, the highest concentrations of both vinculin and talin measured by enzyme-linked immunosorbent assay and intensity of immunofluorescence staining were on 30 V nanotubes. On the other hand, the highest concentrations of ALP, type I collagen, and osteopontin were found on 10 V and 20 V samples. The final cellular densities on 10 V, 20 V, and 30 V samples were higher than on glass. Therefore, the controlled anodization of Ti-6Al-4V seems to be a useful tool for preparing nanostructured materials with desirable biological properties.

No MeSH data available.


Related in: MedlinePlus

Densities and viability of human Saos-2 osteoblasts on 10 V, 20 V, and 30 V nanotubes.Notes: Control Ti_C and glass coverslips on days 1 (A), 3 (B), 7 (C), and cell growth curves on these surfaces (D). Data expressed as mean ± standard error of mean from six measurements. P≤0.05 considered significant in comparison with samples labeled above columns.
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f5-ijn-10-7145: Densities and viability of human Saos-2 osteoblasts on 10 V, 20 V, and 30 V nanotubes.Notes: Control Ti_C and glass coverslips on days 1 (A), 3 (B), 7 (C), and cell growth curves on these surfaces (D). Data expressed as mean ± standard error of mean from six measurements. P≤0.05 considered significant in comparison with samples labeled above columns.

Mentions: At 24 hours after seeding, Saos-2 osteoblasts adhered at the highest density on the control glass surface and on smooth Ti_C. Cells adhered in significantly lower initial densities on Ti-6A1-4V-based nanotubes (Figure 5A). Nevertheless, the Saos-2 cells on all tested surfaces were well spread and polygonal, and their viability was above 96%. On day 3 after seeding, cell densities were similar on all samples, with a viability of approximately 97%–98% (Figure 5B). On day 7, the highest density of Saos-2 cells was observed on smooth Ti_C, and the lowest cell density on glass (Figure 5C and D). Cell densities on 10 V and 30 V nanotube samples were higher than on glass.


The diameter of nanotubes formed on Ti-6Al-4V alloy controls the adhesion and differentiation of Saos-2 cells.

Filova E, Fojt J, Kryslova M, Moravec H, Joska L, Bacakova L - Int J Nanomedicine (2015)

Densities and viability of human Saos-2 osteoblasts on 10 V, 20 V, and 30 V nanotubes.Notes: Control Ti_C and glass coverslips on days 1 (A), 3 (B), 7 (C), and cell growth curves on these surfaces (D). Data expressed as mean ± standard error of mean from six measurements. P≤0.05 considered significant in comparison with samples labeled above columns.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4664495&req=5

f5-ijn-10-7145: Densities and viability of human Saos-2 osteoblasts on 10 V, 20 V, and 30 V nanotubes.Notes: Control Ti_C and glass coverslips on days 1 (A), 3 (B), 7 (C), and cell growth curves on these surfaces (D). Data expressed as mean ± standard error of mean from six measurements. P≤0.05 considered significant in comparison with samples labeled above columns.
Mentions: At 24 hours after seeding, Saos-2 osteoblasts adhered at the highest density on the control glass surface and on smooth Ti_C. Cells adhered in significantly lower initial densities on Ti-6A1-4V-based nanotubes (Figure 5A). Nevertheless, the Saos-2 cells on all tested surfaces were well spread and polygonal, and their viability was above 96%. On day 3 after seeding, cell densities were similar on all samples, with a viability of approximately 97%–98% (Figure 5B). On day 7, the highest density of Saos-2 cells was observed on smooth Ti_C, and the lowest cell density on glass (Figure 5C and D). Cell densities on 10 V and 30 V nanotube samples were higher than on glass.

Bottom Line: On day 3, the highest concentrations of both vinculin and talin measured by enzyme-linked immunosorbent assay and intensity of immunofluorescence staining were on 30 V nanotubes.On the other hand, the highest concentrations of ALP, type I collagen, and osteopontin were found on 10 V and 20 V samples.Therefore, the controlled anodization of Ti-6Al-4V seems to be a useful tool for preparing nanostructured materials with desirable biological properties.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomaterials and Tissue Engineering, Institute of Physiology, Czech Academy of Sciences, Prague, Czech Republic.

ABSTRACT
Ti-6Al-4V-based nanotubes were prepared on a Ti-6Al-4V surface by anodic oxidation on 10 V, 20 V, and 30 V samples. The 10 V, 20 V, and 30 V samples and a control smooth Ti-6Al-4V sample were evaluated in terms of their chemical composition, diameter distribution, and cellular response. The surfaces of the 10 V, 20 V, and 30 V samples consisted of nanotubes of a relatively wide range of diameters that increased with the voltage. Saos-2 cells had a similar initial adhesion on all nanotube samples to the control Ti-6Al-4V sample, but it was lower than on glass. On day 3, the highest concentrations of both vinculin and talin measured by enzyme-linked immunosorbent assay and intensity of immunofluorescence staining were on 30 V nanotubes. On the other hand, the highest concentrations of ALP, type I collagen, and osteopontin were found on 10 V and 20 V samples. The final cellular densities on 10 V, 20 V, and 30 V samples were higher than on glass. Therefore, the controlled anodization of Ti-6Al-4V seems to be a useful tool for preparing nanostructured materials with desirable biological properties.

No MeSH data available.


Related in: MedlinePlus