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Adoptive transfer of immune subsets prior to MCAO does not exacerbate stroke outcome in splenectomized mice.

Wang J, Dotson AL, Murphy SJ, Offner H, Saugstad JA - J Syst Integr Neurosci (2015)

Bottom Line: The results demonstrate that CD4/CD8/CD11b treated mice had no significant effect on infarct volumes vs. vehicle-treated control mice after MCAO.However, there were significant alterations to the resident peripheral immune composition.These results suggest that there are regulatory factors resulting from splenectomy or other possible influences that inhibit peripheral immune cell contribution to neuroinflammation and thus contributing to differential effects of the spleen on stroke outcome in males and female mice.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Anesthesiology and Perioperative Medicine, Oregon Health and Science University, Portland, OR, USA.

ABSTRACT

The peripheral immune response contributes to neurologic impairment after stroke and the extent of initial damage is greater in males than females. We have previously shown that spleen cells directly contribute to ischemic damage in males, as splenectomy prior to experimental stroke eliminates the sex differences in infarct volume. This study aims to determine which specific subset of immune cells exert pathogenic effects when injected 24 hours before MCAO induction into splenectomized male and female WT mice. The results demonstrate that CD4/CD8/CD11b treated mice had no significant effect on infarct volumes vs. vehicle-treated control mice after MCAO. However, there were significant alterations to the resident peripheral immune composition. These results suggest that there are regulatory factors resulting from splenectomy or other possible influences that inhibit peripheral immune cell contribution to neuroinflammation and thus contributing to differential effects of the spleen on stroke outcome in males and female mice.

No MeSH data available.


Related in: MedlinePlus

No effect of adoptive transfer of CD4/CD8 T cells oncerebral blood flow in splenectomized male and female miceMale and female mice were subject to splenectomy two weeks prior to transient MCAO (60 min). Mice were injected with CD4+ or CD8+ T cells 24 hours prior to MCAO. Brains were harvested 96 h after MCAO. (A) Cerebral blood flow measured in male mice injected with saline vehicle (n=10), 12 million CD4+ T cells (n=12), or 8 million CD8+ T cells (n=14) by intravenous transfer 24 h before MCAO. (B) Cerebral blood flow measured in female mice injected with saline vehicle (n=12), 12 million CD4+ T cells (n=11), or 8 million CD8+ T cells (n=11) by intravenous transfer 24 h before MCAO.
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Figure 3: No effect of adoptive transfer of CD4/CD8 T cells oncerebral blood flow in splenectomized male and female miceMale and female mice were subject to splenectomy two weeks prior to transient MCAO (60 min). Mice were injected with CD4+ or CD8+ T cells 24 hours prior to MCAO. Brains were harvested 96 h after MCAO. (A) Cerebral blood flow measured in male mice injected with saline vehicle (n=10), 12 million CD4+ T cells (n=12), or 8 million CD8+ T cells (n=14) by intravenous transfer 24 h before MCAO. (B) Cerebral blood flow measured in female mice injected with saline vehicle (n=12), 12 million CD4+ T cells (n=11), or 8 million CD8+ T cells (n=11) by intravenous transfer 24 h before MCAO.

Mentions: We examined the effect of CD4+ or CD8+ T cell injection 24 hours prior to MCAO in male and female splenectomized mice on cerebral blood flow. Figure 3 shows cerebral blood flow measures for male or female mice before MCAO (baseline), throughout MCAO surgery (5, 15, 30, 45, and 60 min MCAO), and after 5 minutes of reperfusion (5 min Reper). The results show there is no effect of injection with saline vehicle (n = 10), 12 million CD4+ T cells (n = 12), or 8 million CD8+ T cells (n = 14) by intravenous transfer 24 h before MCAO on cerebral blood flow in male mice (Figure 3A). Similarly, the results show that there is no effect of injection of saline vehicle (n = 12), 12 million CD4+ T cells (n = 11), or 8 million CD8+ T cells (n = 11) by intravenous transfer 24 h before MCAO on cerebral blood flow in female mice (Figure 3B).


Adoptive transfer of immune subsets prior to MCAO does not exacerbate stroke outcome in splenectomized mice.

Wang J, Dotson AL, Murphy SJ, Offner H, Saugstad JA - J Syst Integr Neurosci (2015)

No effect of adoptive transfer of CD4/CD8 T cells oncerebral blood flow in splenectomized male and female miceMale and female mice were subject to splenectomy two weeks prior to transient MCAO (60 min). Mice were injected with CD4+ or CD8+ T cells 24 hours prior to MCAO. Brains were harvested 96 h after MCAO. (A) Cerebral blood flow measured in male mice injected with saline vehicle (n=10), 12 million CD4+ T cells (n=12), or 8 million CD8+ T cells (n=14) by intravenous transfer 24 h before MCAO. (B) Cerebral blood flow measured in female mice injected with saline vehicle (n=12), 12 million CD4+ T cells (n=11), or 8 million CD8+ T cells (n=11) by intravenous transfer 24 h before MCAO.
© Copyright Policy - open-access
Related In: Results  -  Collection

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Figure 3: No effect of adoptive transfer of CD4/CD8 T cells oncerebral blood flow in splenectomized male and female miceMale and female mice were subject to splenectomy two weeks prior to transient MCAO (60 min). Mice were injected with CD4+ or CD8+ T cells 24 hours prior to MCAO. Brains were harvested 96 h after MCAO. (A) Cerebral blood flow measured in male mice injected with saline vehicle (n=10), 12 million CD4+ T cells (n=12), or 8 million CD8+ T cells (n=14) by intravenous transfer 24 h before MCAO. (B) Cerebral blood flow measured in female mice injected with saline vehicle (n=12), 12 million CD4+ T cells (n=11), or 8 million CD8+ T cells (n=11) by intravenous transfer 24 h before MCAO.
Mentions: We examined the effect of CD4+ or CD8+ T cell injection 24 hours prior to MCAO in male and female splenectomized mice on cerebral blood flow. Figure 3 shows cerebral blood flow measures for male or female mice before MCAO (baseline), throughout MCAO surgery (5, 15, 30, 45, and 60 min MCAO), and after 5 minutes of reperfusion (5 min Reper). The results show there is no effect of injection with saline vehicle (n = 10), 12 million CD4+ T cells (n = 12), or 8 million CD8+ T cells (n = 14) by intravenous transfer 24 h before MCAO on cerebral blood flow in male mice (Figure 3A). Similarly, the results show that there is no effect of injection of saline vehicle (n = 12), 12 million CD4+ T cells (n = 11), or 8 million CD8+ T cells (n = 11) by intravenous transfer 24 h before MCAO on cerebral blood flow in female mice (Figure 3B).

Bottom Line: The results demonstrate that CD4/CD8/CD11b treated mice had no significant effect on infarct volumes vs. vehicle-treated control mice after MCAO.However, there were significant alterations to the resident peripheral immune composition.These results suggest that there are regulatory factors resulting from splenectomy or other possible influences that inhibit peripheral immune cell contribution to neuroinflammation and thus contributing to differential effects of the spleen on stroke outcome in males and female mice.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Anesthesiology and Perioperative Medicine, Oregon Health and Science University, Portland, OR, USA.

ABSTRACT

The peripheral immune response contributes to neurologic impairment after stroke and the extent of initial damage is greater in males than females. We have previously shown that spleen cells directly contribute to ischemic damage in males, as splenectomy prior to experimental stroke eliminates the sex differences in infarct volume. This study aims to determine which specific subset of immune cells exert pathogenic effects when injected 24 hours before MCAO induction into splenectomized male and female WT mice. The results demonstrate that CD4/CD8/CD11b treated mice had no significant effect on infarct volumes vs. vehicle-treated control mice after MCAO. However, there were significant alterations to the resident peripheral immune composition. These results suggest that there are regulatory factors resulting from splenectomy or other possible influences that inhibit peripheral immune cell contribution to neuroinflammation and thus contributing to differential effects of the spleen on stroke outcome in males and female mice.

No MeSH data available.


Related in: MedlinePlus