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Synthesis, structural characterization, and anticancer activity of a monobenzyltin compound against MCF-7 breast cancer cells.

Fani S, Kamalidehghan B, Lo KM, Hashim NM, Chow KM, Ahmadipour F - Drug Des Devel Ther (2015)

Bottom Line: A significant increase of lactate dehydrogenase release in treated cells was observed via fluorescence analysis.Luminescent analysis showed significant growth in intracellular reactive oxygen species production after treatment.DNA fragmentation was observed as a characteristic of apoptosis in treated cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacy, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.

ABSTRACT
A new monoorganotin Schiff base compound, [N-(3,5-dichloro-2-oxidobenzylidene)-4-chlorobenzyhydrazidato](o-methylbenzyl)aquatin(IV) chloride, (compound C1), was synthesized, and its structural features were investigated by spectroscopic techniques and single-crystal X-ray diffractometry. Compound C1 was exposed to several human cancer cell lines, including breast adenocarcinoma cell lines MCF-7 and MDA-MB-231, ovarian adenocarcinoma cell lines Skov3 and Caov3, and prostate cancer cell line PC3, in order to examine its cytotoxic effect for different forms of cancer. Human hepatic cell line WRL-68 was used as a normal cell line. We concentrated on the MCF-7 cell line to detect possible underlying mechanism involvement of compound C1. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay revealed the strongest cytotoxicity of compound C1 against MCF-7 cells, with a half maximal inhibitory concentration (IC50) value of 2.5±0.50 μg/mL after 48 hours treatment. The IC50 value was >30 μg/mL in WRL-68 cells. Induced antiproliferative activity of compound C1 for MCF-7 cells was further confirmed by lactate dehydrogenase, reactive oxygen species, acridine orange/propidium iodide staining, and DNA fragmentation assays. A significant increase of lactate dehydrogenase release in treated cells was observed via fluorescence analysis. Luminescent analysis showed significant growth in intracellular reactive oxygen species production after treatment. Morphological changes of necrosis and early and late apoptosis stages were observed in treated cells after staining with acridine orange/propidium iodide. DNA fragmentation was observed as a characteristic of apoptosis in treated cells. Results of the present study obviously reveal potential cytotoxic effects of compound C1 against human breast cancer MCF-7 cells.

No MeSH data available.


Related in: MedlinePlus

Agarose gel image of ladder formation.Notes: MCF-7 cells were exposed to benzyltin compound C1 at IC50 for 24, 48, and 72 hours. DNA was extracted and fragmentation in DNA was assessed using electrophoresis and 1.5% agarose gel. The occurrence of ladders for treated MCF-7 cells indicates apoptosis.Abbreviations: C1, compound 1 [N-(3,5-dichloro-2-oxidobenzylidene)-4-chloro-benzyhydrazidato](o-methylbenzyl)aquatin(IV) chloride; h, hours; IC50, half maximal inhibitory concentration.
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f8-dddt-9-6191: Agarose gel image of ladder formation.Notes: MCF-7 cells were exposed to benzyltin compound C1 at IC50 for 24, 48, and 72 hours. DNA was extracted and fragmentation in DNA was assessed using electrophoresis and 1.5% agarose gel. The occurrence of ladders for treated MCF-7 cells indicates apoptosis.Abbreviations: C1, compound 1 [N-(3,5-dichloro-2-oxidobenzylidene)-4-chloro-benzyhydrazidato](o-methylbenzyl)aquatin(IV) chloride; h, hours; IC50, half maximal inhibitory concentration.

Mentions: The DNA ladder, a form of DNA degradation, is acknowledged as a key biomedical pattern of apoptosis. Our results show that the internucleosomal fragmentation was pronounced in compound C1-treated MCF-7 samples. As can be observed in Figure 8, creation of the DNA ladder happened following exposure of cells to compound for 24, 48, and 72 hours. The positive control in Figure 8 demonstrates the ladder of the DNA.


Synthesis, structural characterization, and anticancer activity of a monobenzyltin compound against MCF-7 breast cancer cells.

Fani S, Kamalidehghan B, Lo KM, Hashim NM, Chow KM, Ahmadipour F - Drug Des Devel Ther (2015)

Agarose gel image of ladder formation.Notes: MCF-7 cells were exposed to benzyltin compound C1 at IC50 for 24, 48, and 72 hours. DNA was extracted and fragmentation in DNA was assessed using electrophoresis and 1.5% agarose gel. The occurrence of ladders for treated MCF-7 cells indicates apoptosis.Abbreviations: C1, compound 1 [N-(3,5-dichloro-2-oxidobenzylidene)-4-chloro-benzyhydrazidato](o-methylbenzyl)aquatin(IV) chloride; h, hours; IC50, half maximal inhibitory concentration.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4664434&req=5

f8-dddt-9-6191: Agarose gel image of ladder formation.Notes: MCF-7 cells were exposed to benzyltin compound C1 at IC50 for 24, 48, and 72 hours. DNA was extracted and fragmentation in DNA was assessed using electrophoresis and 1.5% agarose gel. The occurrence of ladders for treated MCF-7 cells indicates apoptosis.Abbreviations: C1, compound 1 [N-(3,5-dichloro-2-oxidobenzylidene)-4-chloro-benzyhydrazidato](o-methylbenzyl)aquatin(IV) chloride; h, hours; IC50, half maximal inhibitory concentration.
Mentions: The DNA ladder, a form of DNA degradation, is acknowledged as a key biomedical pattern of apoptosis. Our results show that the internucleosomal fragmentation was pronounced in compound C1-treated MCF-7 samples. As can be observed in Figure 8, creation of the DNA ladder happened following exposure of cells to compound for 24, 48, and 72 hours. The positive control in Figure 8 demonstrates the ladder of the DNA.

Bottom Line: A significant increase of lactate dehydrogenase release in treated cells was observed via fluorescence analysis.Luminescent analysis showed significant growth in intracellular reactive oxygen species production after treatment.DNA fragmentation was observed as a characteristic of apoptosis in treated cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacy, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.

ABSTRACT
A new monoorganotin Schiff base compound, [N-(3,5-dichloro-2-oxidobenzylidene)-4-chlorobenzyhydrazidato](o-methylbenzyl)aquatin(IV) chloride, (compound C1), was synthesized, and its structural features were investigated by spectroscopic techniques and single-crystal X-ray diffractometry. Compound C1 was exposed to several human cancer cell lines, including breast adenocarcinoma cell lines MCF-7 and MDA-MB-231, ovarian adenocarcinoma cell lines Skov3 and Caov3, and prostate cancer cell line PC3, in order to examine its cytotoxic effect for different forms of cancer. Human hepatic cell line WRL-68 was used as a normal cell line. We concentrated on the MCF-7 cell line to detect possible underlying mechanism involvement of compound C1. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay revealed the strongest cytotoxicity of compound C1 against MCF-7 cells, with a half maximal inhibitory concentration (IC50) value of 2.5±0.50 μg/mL after 48 hours treatment. The IC50 value was >30 μg/mL in WRL-68 cells. Induced antiproliferative activity of compound C1 for MCF-7 cells was further confirmed by lactate dehydrogenase, reactive oxygen species, acridine orange/propidium iodide staining, and DNA fragmentation assays. A significant increase of lactate dehydrogenase release in treated cells was observed via fluorescence analysis. Luminescent analysis showed significant growth in intracellular reactive oxygen species production after treatment. Morphological changes of necrosis and early and late apoptosis stages were observed in treated cells after staining with acridine orange/propidium iodide. DNA fragmentation was observed as a characteristic of apoptosis in treated cells. Results of the present study obviously reveal potential cytotoxic effects of compound C1 against human breast cancer MCF-7 cells.

No MeSH data available.


Related in: MedlinePlus