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Genome Sequence of African Swine Fever Virus BA71, the Virulent Parental Strain of the Nonpathogenic and Tissue-Culture Adapted BA71V.

Rodríguez JM, Moreno LT, Alejo A, Lacasta A, Rodríguez F, Salas ML - PLoS ONE (2015)

Bottom Line: They possess the smallest genomes for a virulent or an attenuated ASFV, and are essentially identical except for a relatively small number of changes.We discuss the possible contribution of these changes to virulence.Analysis of the BA71 sequence allowed us to identify new similarities among ASFV proteins, and with database proteins including two ASFV proteins that could function as a two-component signaling network.

View Article: PubMed Central - PubMed

Affiliation: Centro Nacional de Microbiología, Instituto Nacional de Salud Carlos III, Majadahonda, Madrid, Spain.

ABSTRACT
The strain BA71V has played a key role in African swine fever virus (ASFV) research. It was the first genome sequenced, and remains the only genome completely determined. A large part of the studies on the function of ASFV genes, viral transcription, replication, DNA repair and morphogenesis, has been performed using this model. This avirulent strain was obtained by adaptation to grow in Vero cells of the highly virulent BA71 strain. We report here the analysis of the genome sequence of BA71 in comparison with that of BA71V. They possess the smallest genomes for a virulent or an attenuated ASFV, and are essentially identical except for a relatively small number of changes. We discuss the possible contribution of these changes to virulence. Analysis of the BA71 sequence allowed us to identify new similarities among ASFV proteins, and with database proteins including two ASFV proteins that could function as a two-component signaling network.

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Related in: MedlinePlus

Comparison of the genomic structure of BA71 with BA71V and OURT88/3 around difference 49.The figure shows a representation to scale of the genomes of BA71, BA71V and OURT88/3 around the position of BA71-BA71V difference 49 (the exact positions are indicated for each of the genomes). Red arrows delimit non-identical regions. Blue lines connect arrows at equivalent positions. The different groups of ORFs are identified by colors as shown in the figure. The dash at the beginning of the names of some ORFs indicates where the prefix BA71- has been removed to avoid clutter.
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pone.0142889.g006: Comparison of the genomic structure of BA71 with BA71V and OURT88/3 around difference 49.The figure shows a representation to scale of the genomes of BA71, BA71V and OURT88/3 around the position of BA71-BA71V difference 49 (the exact positions are indicated for each of the genomes). Red arrows delimit non-identical regions. Blue lines connect arrows at equivalent positions. The different groups of ORFs are identified by colors as shown in the figure. The dash at the beginning of the names of some ORFs indicates where the prefix BA71- has been removed to avoid clutter.

Mentions: Finally, the fourth large deletion, d49, in BA71V affects the 3’-end of the genome (Table 2, Fig 6, S5 Fig) in a region that contains members of the MGF100, P22 and MGF360 multigene families. This region is variable among virulent isolates but all of them maintain the minimal set of genes found in BA71, Lisbon60 or Georgia2007/1. This set includes two members of the multigene family 100 (BA71-M102L, -M103L), a member of the MGF P22 (BA71-J170L), the gene BA71-M98R, which is highly similar to the ASFV gene L83L (S1 Fig) but has no other detectable similarities to database sequences, and the gene BA71-J93L, which also has no known similarities. Deletions in this region are only observed in BA71V and in the Vero cell adapted Georgia G/VP110 strain [19], suggesting that although the deletion in d15 appears to be the main cause of the attenuation, this deletion may contribute to the extent of attenuation found in these highly attenuated strains.


Genome Sequence of African Swine Fever Virus BA71, the Virulent Parental Strain of the Nonpathogenic and Tissue-Culture Adapted BA71V.

Rodríguez JM, Moreno LT, Alejo A, Lacasta A, Rodríguez F, Salas ML - PLoS ONE (2015)

Comparison of the genomic structure of BA71 with BA71V and OURT88/3 around difference 49.The figure shows a representation to scale of the genomes of BA71, BA71V and OURT88/3 around the position of BA71-BA71V difference 49 (the exact positions are indicated for each of the genomes). Red arrows delimit non-identical regions. Blue lines connect arrows at equivalent positions. The different groups of ORFs are identified by colors as shown in the figure. The dash at the beginning of the names of some ORFs indicates where the prefix BA71- has been removed to avoid clutter.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4664411&req=5

pone.0142889.g006: Comparison of the genomic structure of BA71 with BA71V and OURT88/3 around difference 49.The figure shows a representation to scale of the genomes of BA71, BA71V and OURT88/3 around the position of BA71-BA71V difference 49 (the exact positions are indicated for each of the genomes). Red arrows delimit non-identical regions. Blue lines connect arrows at equivalent positions. The different groups of ORFs are identified by colors as shown in the figure. The dash at the beginning of the names of some ORFs indicates where the prefix BA71- has been removed to avoid clutter.
Mentions: Finally, the fourth large deletion, d49, in BA71V affects the 3’-end of the genome (Table 2, Fig 6, S5 Fig) in a region that contains members of the MGF100, P22 and MGF360 multigene families. This region is variable among virulent isolates but all of them maintain the minimal set of genes found in BA71, Lisbon60 or Georgia2007/1. This set includes two members of the multigene family 100 (BA71-M102L, -M103L), a member of the MGF P22 (BA71-J170L), the gene BA71-M98R, which is highly similar to the ASFV gene L83L (S1 Fig) but has no other detectable similarities to database sequences, and the gene BA71-J93L, which also has no known similarities. Deletions in this region are only observed in BA71V and in the Vero cell adapted Georgia G/VP110 strain [19], suggesting that although the deletion in d15 appears to be the main cause of the attenuation, this deletion may contribute to the extent of attenuation found in these highly attenuated strains.

Bottom Line: They possess the smallest genomes for a virulent or an attenuated ASFV, and are essentially identical except for a relatively small number of changes.We discuss the possible contribution of these changes to virulence.Analysis of the BA71 sequence allowed us to identify new similarities among ASFV proteins, and with database proteins including two ASFV proteins that could function as a two-component signaling network.

View Article: PubMed Central - PubMed

Affiliation: Centro Nacional de Microbiología, Instituto Nacional de Salud Carlos III, Majadahonda, Madrid, Spain.

ABSTRACT
The strain BA71V has played a key role in African swine fever virus (ASFV) research. It was the first genome sequenced, and remains the only genome completely determined. A large part of the studies on the function of ASFV genes, viral transcription, replication, DNA repair and morphogenesis, has been performed using this model. This avirulent strain was obtained by adaptation to grow in Vero cells of the highly virulent BA71 strain. We report here the analysis of the genome sequence of BA71 in comparison with that of BA71V. They possess the smallest genomes for a virulent or an attenuated ASFV, and are essentially identical except for a relatively small number of changes. We discuss the possible contribution of these changes to virulence. Analysis of the BA71 sequence allowed us to identify new similarities among ASFV proteins, and with database proteins including two ASFV proteins that could function as a two-component signaling network.

Show MeSH
Related in: MedlinePlus