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Genome Sequence of African Swine Fever Virus BA71, the Virulent Parental Strain of the Nonpathogenic and Tissue-Culture Adapted BA71V.

Rodríguez JM, Moreno LT, Alejo A, Lacasta A, Rodríguez F, Salas ML - PLoS ONE (2015)

Bottom Line: They possess the smallest genomes for a virulent or an attenuated ASFV, and are essentially identical except for a relatively small number of changes.We discuss the possible contribution of these changes to virulence.Analysis of the BA71 sequence allowed us to identify new similarities among ASFV proteins, and with database proteins including two ASFV proteins that could function as a two-component signaling network.

View Article: PubMed Central - PubMed

Affiliation: Centro Nacional de Microbiología, Instituto Nacional de Salud Carlos III, Majadahonda, Madrid, Spain.

ABSTRACT
The strain BA71V has played a key role in African swine fever virus (ASFV) research. It was the first genome sequenced, and remains the only genome completely determined. A large part of the studies on the function of ASFV genes, viral transcription, replication, DNA repair and morphogenesis, has been performed using this model. This avirulent strain was obtained by adaptation to grow in Vero cells of the highly virulent BA71 strain. We report here the analysis of the genome sequence of BA71 in comparison with that of BA71V. They possess the smallest genomes for a virulent or an attenuated ASFV, and are essentially identical except for a relatively small number of changes. We discuss the possible contribution of these changes to virulence. Analysis of the BA71 sequence allowed us to identify new similarities among ASFV proteins, and with database proteins including two ASFV proteins that could function as a two-component signaling network.

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Comparison of the genomic structure of BA71 with BA71V and OURT88/3 around difference 17.The figure shows a representation to scale of the genomes of BA71, BA71V and OURT88/3 around the position of BA71-BA71V difference 17 (the exact positions are indicated for each of the genomes). Red arrows delimit non-identical regions. Blue lines connect arrows at equivalent positions. The ORFs are members of MGF 505.
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pone.0142889.g005: Comparison of the genomic structure of BA71 with BA71V and OURT88/3 around difference 17.The figure shows a representation to scale of the genomes of BA71, BA71V and OURT88/3 around the position of BA71-BA71V difference 17 (the exact positions are indicated for each of the genomes). Red arrows delimit non-identical regions. Blue lines connect arrows at equivalent positions. The ORFs are members of MGF 505.

Mentions: The third large deletion, d17 (Table 2, Fig 5, S4 Fig), appears to be the product of a recombination event between two members of the BA71 MGF 505, BA71-A528R and BA71-A469R. In BA71V the gene A528R corresponds to the fusion of the 5’-half of gene BA71-A528R (orthologous to MGF 505-7R) with the 3’-half of gene BA71-A469R (orthologous to MGF 505-8R). A similar genetic structure than that of BA71V is observed in the virulent Malawi Lil-20/1 and Kenya 1950 isolates, whereas the attenuated OURT88/3 possesses a structure similar to that of BA71, or Lisbon60; thus it seems unlikely that this deletion is related to virulence.


Genome Sequence of African Swine Fever Virus BA71, the Virulent Parental Strain of the Nonpathogenic and Tissue-Culture Adapted BA71V.

Rodríguez JM, Moreno LT, Alejo A, Lacasta A, Rodríguez F, Salas ML - PLoS ONE (2015)

Comparison of the genomic structure of BA71 with BA71V and OURT88/3 around difference 17.The figure shows a representation to scale of the genomes of BA71, BA71V and OURT88/3 around the position of BA71-BA71V difference 17 (the exact positions are indicated for each of the genomes). Red arrows delimit non-identical regions. Blue lines connect arrows at equivalent positions. The ORFs are members of MGF 505.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4664411&req=5

pone.0142889.g005: Comparison of the genomic structure of BA71 with BA71V and OURT88/3 around difference 17.The figure shows a representation to scale of the genomes of BA71, BA71V and OURT88/3 around the position of BA71-BA71V difference 17 (the exact positions are indicated for each of the genomes). Red arrows delimit non-identical regions. Blue lines connect arrows at equivalent positions. The ORFs are members of MGF 505.
Mentions: The third large deletion, d17 (Table 2, Fig 5, S4 Fig), appears to be the product of a recombination event between two members of the BA71 MGF 505, BA71-A528R and BA71-A469R. In BA71V the gene A528R corresponds to the fusion of the 5’-half of gene BA71-A528R (orthologous to MGF 505-7R) with the 3’-half of gene BA71-A469R (orthologous to MGF 505-8R). A similar genetic structure than that of BA71V is observed in the virulent Malawi Lil-20/1 and Kenya 1950 isolates, whereas the attenuated OURT88/3 possesses a structure similar to that of BA71, or Lisbon60; thus it seems unlikely that this deletion is related to virulence.

Bottom Line: They possess the smallest genomes for a virulent or an attenuated ASFV, and are essentially identical except for a relatively small number of changes.We discuss the possible contribution of these changes to virulence.Analysis of the BA71 sequence allowed us to identify new similarities among ASFV proteins, and with database proteins including two ASFV proteins that could function as a two-component signaling network.

View Article: PubMed Central - PubMed

Affiliation: Centro Nacional de Microbiología, Instituto Nacional de Salud Carlos III, Majadahonda, Madrid, Spain.

ABSTRACT
The strain BA71V has played a key role in African swine fever virus (ASFV) research. It was the first genome sequenced, and remains the only genome completely determined. A large part of the studies on the function of ASFV genes, viral transcription, replication, DNA repair and morphogenesis, has been performed using this model. This avirulent strain was obtained by adaptation to grow in Vero cells of the highly virulent BA71 strain. We report here the analysis of the genome sequence of BA71 in comparison with that of BA71V. They possess the smallest genomes for a virulent or an attenuated ASFV, and are essentially identical except for a relatively small number of changes. We discuss the possible contribution of these changes to virulence. Analysis of the BA71 sequence allowed us to identify new similarities among ASFV proteins, and with database proteins including two ASFV proteins that could function as a two-component signaling network.

Show MeSH
Related in: MedlinePlus