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Genome Sequence of African Swine Fever Virus BA71, the Virulent Parental Strain of the Nonpathogenic and Tissue-Culture Adapted BA71V.

Rodríguez JM, Moreno LT, Alejo A, Lacasta A, Rodríguez F, Salas ML - PLoS ONE (2015)

Bottom Line: They possess the smallest genomes for a virulent or an attenuated ASFV, and are essentially identical except for a relatively small number of changes.We discuss the possible contribution of these changes to virulence.Analysis of the BA71 sequence allowed us to identify new similarities among ASFV proteins, and with database proteins including two ASFV proteins that could function as a two-component signaling network.

View Article: PubMed Central - PubMed

Affiliation: Centro Nacional de Microbiología, Instituto Nacional de Salud Carlos III, Majadahonda, Madrid, Spain.

ABSTRACT
The strain BA71V has played a key role in African swine fever virus (ASFV) research. It was the first genome sequenced, and remains the only genome completely determined. A large part of the studies on the function of ASFV genes, viral transcription, replication, DNA repair and morphogenesis, has been performed using this model. This avirulent strain was obtained by adaptation to grow in Vero cells of the highly virulent BA71 strain. We report here the analysis of the genome sequence of BA71 in comparison with that of BA71V. They possess the smallest genomes for a virulent or an attenuated ASFV, and are essentially identical except for a relatively small number of changes. We discuss the possible contribution of these changes to virulence. Analysis of the BA71 sequence allowed us to identify new similarities among ASFV proteins, and with database proteins including two ASFV proteins that could function as a two-component signaling network.

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Related in: MedlinePlus

Comparison of the genomic structure of BA71 with BA71V and OURT88/3 around difference 15.The figure shows a representation to scale of the genomes of BA71, BA71V and OURT88/3 around the position of BA71-BA71V difference 15 (the exact positions are indicated for each of the genomes). Red arrows delimit non-identical regions. Blue lines connect arrows at equivalent positions. The different groups of ORFs are identified by colors as indicated in the figure.
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pone.0142889.g004: Comparison of the genomic structure of BA71 with BA71V and OURT88/3 around difference 15.The figure shows a representation to scale of the genomes of BA71, BA71V and OURT88/3 around the position of BA71-BA71V difference 15 (the exact positions are indicated for each of the genomes). Red arrows delimit non-identical regions. Blue lines connect arrows at equivalent positions. The different groups of ORFs are identified by colors as indicated in the figure.

Mentions: In contrast with the other differences, d15 (Table 2, Fig 4, S3 Fig), the largest deletion, occurs only in attenuated strains, thus all the sequenced genomes of virulent viruses possess a conserved genomic structure in this area, whereas all the attenuated non-recombinant viruses possess deletions in this region [9–11,19,56]. Experimental results are in agreement with the existence of a link between attenuation and the deletion of MGF360 and -505 in this region [2,10,19,20]. There is less agreement, however, about a possible role for the genes encoded in this region and the ability of the virus to replicate in macrophages. Thus, whereas in the attenuated BA71V replication in macrophages could be rescued by the insertion of three of the MGF360 genes of his region [9], OURT88/3, which possesses a larger deletion in this region than BA71V (Fig 4), was shown to efficiently replicate in macrophages [57]. Also, the deletion mutant E70∆NL-S, that did not show any defect on macrophage replication [14], was later shown to have a deletion in this region very similar to that of BA71V [10]. Finally, conflicting evidence has been shown about the ability of the strain BA71V to grow in macrophages [9,58,59].


Genome Sequence of African Swine Fever Virus BA71, the Virulent Parental Strain of the Nonpathogenic and Tissue-Culture Adapted BA71V.

Rodríguez JM, Moreno LT, Alejo A, Lacasta A, Rodríguez F, Salas ML - PLoS ONE (2015)

Comparison of the genomic structure of BA71 with BA71V and OURT88/3 around difference 15.The figure shows a representation to scale of the genomes of BA71, BA71V and OURT88/3 around the position of BA71-BA71V difference 15 (the exact positions are indicated for each of the genomes). Red arrows delimit non-identical regions. Blue lines connect arrows at equivalent positions. The different groups of ORFs are identified by colors as indicated in the figure.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4664411&req=5

pone.0142889.g004: Comparison of the genomic structure of BA71 with BA71V and OURT88/3 around difference 15.The figure shows a representation to scale of the genomes of BA71, BA71V and OURT88/3 around the position of BA71-BA71V difference 15 (the exact positions are indicated for each of the genomes). Red arrows delimit non-identical regions. Blue lines connect arrows at equivalent positions. The different groups of ORFs are identified by colors as indicated in the figure.
Mentions: In contrast with the other differences, d15 (Table 2, Fig 4, S3 Fig), the largest deletion, occurs only in attenuated strains, thus all the sequenced genomes of virulent viruses possess a conserved genomic structure in this area, whereas all the attenuated non-recombinant viruses possess deletions in this region [9–11,19,56]. Experimental results are in agreement with the existence of a link between attenuation and the deletion of MGF360 and -505 in this region [2,10,19,20]. There is less agreement, however, about a possible role for the genes encoded in this region and the ability of the virus to replicate in macrophages. Thus, whereas in the attenuated BA71V replication in macrophages could be rescued by the insertion of three of the MGF360 genes of his region [9], OURT88/3, which possesses a larger deletion in this region than BA71V (Fig 4), was shown to efficiently replicate in macrophages [57]. Also, the deletion mutant E70∆NL-S, that did not show any defect on macrophage replication [14], was later shown to have a deletion in this region very similar to that of BA71V [10]. Finally, conflicting evidence has been shown about the ability of the strain BA71V to grow in macrophages [9,58,59].

Bottom Line: They possess the smallest genomes for a virulent or an attenuated ASFV, and are essentially identical except for a relatively small number of changes.We discuss the possible contribution of these changes to virulence.Analysis of the BA71 sequence allowed us to identify new similarities among ASFV proteins, and with database proteins including two ASFV proteins that could function as a two-component signaling network.

View Article: PubMed Central - PubMed

Affiliation: Centro Nacional de Microbiología, Instituto Nacional de Salud Carlos III, Majadahonda, Madrid, Spain.

ABSTRACT
The strain BA71V has played a key role in African swine fever virus (ASFV) research. It was the first genome sequenced, and remains the only genome completely determined. A large part of the studies on the function of ASFV genes, viral transcription, replication, DNA repair and morphogenesis, has been performed using this model. This avirulent strain was obtained by adaptation to grow in Vero cells of the highly virulent BA71 strain. We report here the analysis of the genome sequence of BA71 in comparison with that of BA71V. They possess the smallest genomes for a virulent or an attenuated ASFV, and are essentially identical except for a relatively small number of changes. We discuss the possible contribution of these changes to virulence. Analysis of the BA71 sequence allowed us to identify new similarities among ASFV proteins, and with database proteins including two ASFV proteins that could function as a two-component signaling network.

Show MeSH
Related in: MedlinePlus