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Genome Sequence of African Swine Fever Virus BA71, the Virulent Parental Strain of the Nonpathogenic and Tissue-Culture Adapted BA71V.

Rodríguez JM, Moreno LT, Alejo A, Lacasta A, Rodríguez F, Salas ML - PLoS ONE (2015)

Bottom Line: They possess the smallest genomes for a virulent or an attenuated ASFV, and are essentially identical except for a relatively small number of changes.We discuss the possible contribution of these changes to virulence.Analysis of the BA71 sequence allowed us to identify new similarities among ASFV proteins, and with database proteins including two ASFV proteins that could function as a two-component signaling network.

View Article: PubMed Central - PubMed

Affiliation: Centro Nacional de Microbiología, Instituto Nacional de Salud Carlos III, Majadahonda, Madrid, Spain.

ABSTRACT
The strain BA71V has played a key role in African swine fever virus (ASFV) research. It was the first genome sequenced, and remains the only genome completely determined. A large part of the studies on the function of ASFV genes, viral transcription, replication, DNA repair and morphogenesis, has been performed using this model. This avirulent strain was obtained by adaptation to grow in Vero cells of the highly virulent BA71 strain. We report here the analysis of the genome sequence of BA71 in comparison with that of BA71V. They possess the smallest genomes for a virulent or an attenuated ASFV, and are essentially identical except for a relatively small number of changes. We discuss the possible contribution of these changes to virulence. Analysis of the BA71 sequence allowed us to identify new similarities among ASFV proteins, and with database proteins including two ASFV proteins that could function as a two-component signaling network.

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Comparison of the genomic structure of BA71 with BA71V and OURT88/3 around difference 9.The figure shows a representation to scale of the genomes of BA71, BA71V and OURT88/3 around the position of BA71-BA71V difference 9 (the exact positions are indicated for each of the genomes). Red arrows delimit non-identical regions, which are indicated by a central red line in the BA71V and OURT88/3 genomes. Blue lines connect arrows at equivalent positions. The different groups of ORFs are identified by colors as indicated in the figure.
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pone.0142889.g003: Comparison of the genomic structure of BA71 with BA71V and OURT88/3 around difference 9.The figure shows a representation to scale of the genomes of BA71, BA71V and OURT88/3 around the position of BA71-BA71V difference 9 (the exact positions are indicated for each of the genomes). Red arrows delimit non-identical regions, which are indicated by a central red line in the BA71V and OURT88/3 genomes. Blue lines connect arrows at equivalent positions. The different groups of ORFs are identified by colors as indicated in the figure.

Mentions: The first large deletion, d9, corresponds to a difference of 1354 nts in the MGF110 region of the LVR of the genomes (Fig 3, S2 Fig). The different sequence includes two genes, UP60L, member of the MGF360, and X69R, a small protein of unknown function, similar to J64R as indicated previously (S1 Fig), which is maintained in the genome of all sequenced viruses. These sequences are deleted in the virulent BA71 but conserved in the attenuated BA71V, thus very unlikely related to virulence. This deletion in the BA71 genome probably occurred during the passages given to BA71 after the sample used to generate BA71V was separated [26]. This region varies widely among isolates, without regard to their virulence, due principally to changes in the number of genes of MGF100, -110 and -360. Interestingly, BA71 possesses the smaller genetic complement in this region, only 6 genes. This compares with the 16 genes present in the Georgia2007/1 isolate in the same region.


Genome Sequence of African Swine Fever Virus BA71, the Virulent Parental Strain of the Nonpathogenic and Tissue-Culture Adapted BA71V.

Rodríguez JM, Moreno LT, Alejo A, Lacasta A, Rodríguez F, Salas ML - PLoS ONE (2015)

Comparison of the genomic structure of BA71 with BA71V and OURT88/3 around difference 9.The figure shows a representation to scale of the genomes of BA71, BA71V and OURT88/3 around the position of BA71-BA71V difference 9 (the exact positions are indicated for each of the genomes). Red arrows delimit non-identical regions, which are indicated by a central red line in the BA71V and OURT88/3 genomes. Blue lines connect arrows at equivalent positions. The different groups of ORFs are identified by colors as indicated in the figure.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4664411&req=5

pone.0142889.g003: Comparison of the genomic structure of BA71 with BA71V and OURT88/3 around difference 9.The figure shows a representation to scale of the genomes of BA71, BA71V and OURT88/3 around the position of BA71-BA71V difference 9 (the exact positions are indicated for each of the genomes). Red arrows delimit non-identical regions, which are indicated by a central red line in the BA71V and OURT88/3 genomes. Blue lines connect arrows at equivalent positions. The different groups of ORFs are identified by colors as indicated in the figure.
Mentions: The first large deletion, d9, corresponds to a difference of 1354 nts in the MGF110 region of the LVR of the genomes (Fig 3, S2 Fig). The different sequence includes two genes, UP60L, member of the MGF360, and X69R, a small protein of unknown function, similar to J64R as indicated previously (S1 Fig), which is maintained in the genome of all sequenced viruses. These sequences are deleted in the virulent BA71 but conserved in the attenuated BA71V, thus very unlikely related to virulence. This deletion in the BA71 genome probably occurred during the passages given to BA71 after the sample used to generate BA71V was separated [26]. This region varies widely among isolates, without regard to their virulence, due principally to changes in the number of genes of MGF100, -110 and -360. Interestingly, BA71 possesses the smaller genetic complement in this region, only 6 genes. This compares with the 16 genes present in the Georgia2007/1 isolate in the same region.

Bottom Line: They possess the smallest genomes for a virulent or an attenuated ASFV, and are essentially identical except for a relatively small number of changes.We discuss the possible contribution of these changes to virulence.Analysis of the BA71 sequence allowed us to identify new similarities among ASFV proteins, and with database proteins including two ASFV proteins that could function as a two-component signaling network.

View Article: PubMed Central - PubMed

Affiliation: Centro Nacional de Microbiología, Instituto Nacional de Salud Carlos III, Majadahonda, Madrid, Spain.

ABSTRACT
The strain BA71V has played a key role in African swine fever virus (ASFV) research. It was the first genome sequenced, and remains the only genome completely determined. A large part of the studies on the function of ASFV genes, viral transcription, replication, DNA repair and morphogenesis, has been performed using this model. This avirulent strain was obtained by adaptation to grow in Vero cells of the highly virulent BA71 strain. We report here the analysis of the genome sequence of BA71 in comparison with that of BA71V. They possess the smallest genomes for a virulent or an attenuated ASFV, and are essentially identical except for a relatively small number of changes. We discuss the possible contribution of these changes to virulence. Analysis of the BA71 sequence allowed us to identify new similarities among ASFV proteins, and with database proteins including two ASFV proteins that could function as a two-component signaling network.

Show MeSH
Related in: MedlinePlus