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Genome Sequence of African Swine Fever Virus BA71, the Virulent Parental Strain of the Nonpathogenic and Tissue-Culture Adapted BA71V.

Rodríguez JM, Moreno LT, Alejo A, Lacasta A, Rodríguez F, Salas ML - PLoS ONE (2015)

Bottom Line: They possess the smallest genomes for a virulent or an attenuated ASFV, and are essentially identical except for a relatively small number of changes.We discuss the possible contribution of these changes to virulence.Analysis of the BA71 sequence allowed us to identify new similarities among ASFV proteins, and with database proteins including two ASFV proteins that could function as a two-component signaling network.

View Article: PubMed Central - PubMed

Affiliation: Centro Nacional de Microbiología, Instituto Nacional de Salud Carlos III, Majadahonda, Madrid, Spain.

ABSTRACT
The strain BA71V has played a key role in African swine fever virus (ASFV) research. It was the first genome sequenced, and remains the only genome completely determined. A large part of the studies on the function of ASFV genes, viral transcription, replication, DNA repair and morphogenesis, has been performed using this model. This avirulent strain was obtained by adaptation to grow in Vero cells of the highly virulent BA71 strain. We report here the analysis of the genome sequence of BA71 in comparison with that of BA71V. They possess the smallest genomes for a virulent or an attenuated ASFV, and are essentially identical except for a relatively small number of changes. We discuss the possible contribution of these changes to virulence. Analysis of the BA71 sequence allowed us to identify new similarities among ASFV proteins, and with database proteins including two ASFV proteins that could function as a two-component signaling network.

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Virulence of ASFV BA71 strain.(A) Rectal temperatures were daily recorded after infection of pigs with 102 or 104 HAU50 of BA71. (B) Survival of pigs after infection with 102 or 104 HAU50 of BA71.
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pone.0142889.g001: Virulence of ASFV BA71 strain.(A) Rectal temperatures were daily recorded after infection of pigs with 102 or 104 HAU50 of BA71. (B) Survival of pigs after infection with 102 or 104 HAU50 of BA71.

Mentions: The isolate of ASFV BA71 maintained in our laboratory derives from the virus used for the characterization of this strain by Enjuanes et al. [26], during which it was passaged about one hundred times in swine monocytes. The BA71V strain was derived using the 36th passage of BA71, by adaptation to grow in Vero cells as described [26]. Thus, from the 36th passage on both viruses have diverged: BA71V suffered further passages for the adaptation to growth in Vero cells, and BA71 was subjected to at least 64 additional passages in swine monocytes, to which we must add the 10 additional passages in porcine alveolar macrophages needed for the cloning and expansion procedures of this work. To confirm the virulence of the BA71 isolate after cloning and expansion in porcine alveolar macrophages, pigs were inoculated with semi-purified virus as described in Materials and Methods and ASF clinical signs, including rectal temperature (Fig 1A), were daily recorded, and the time of death for each infected animal registered (Fig 1B).


Genome Sequence of African Swine Fever Virus BA71, the Virulent Parental Strain of the Nonpathogenic and Tissue-Culture Adapted BA71V.

Rodríguez JM, Moreno LT, Alejo A, Lacasta A, Rodríguez F, Salas ML - PLoS ONE (2015)

Virulence of ASFV BA71 strain.(A) Rectal temperatures were daily recorded after infection of pigs with 102 or 104 HAU50 of BA71. (B) Survival of pigs after infection with 102 or 104 HAU50 of BA71.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4664411&req=5

pone.0142889.g001: Virulence of ASFV BA71 strain.(A) Rectal temperatures were daily recorded after infection of pigs with 102 or 104 HAU50 of BA71. (B) Survival of pigs after infection with 102 or 104 HAU50 of BA71.
Mentions: The isolate of ASFV BA71 maintained in our laboratory derives from the virus used for the characterization of this strain by Enjuanes et al. [26], during which it was passaged about one hundred times in swine monocytes. The BA71V strain was derived using the 36th passage of BA71, by adaptation to grow in Vero cells as described [26]. Thus, from the 36th passage on both viruses have diverged: BA71V suffered further passages for the adaptation to growth in Vero cells, and BA71 was subjected to at least 64 additional passages in swine monocytes, to which we must add the 10 additional passages in porcine alveolar macrophages needed for the cloning and expansion procedures of this work. To confirm the virulence of the BA71 isolate after cloning and expansion in porcine alveolar macrophages, pigs were inoculated with semi-purified virus as described in Materials and Methods and ASF clinical signs, including rectal temperature (Fig 1A), were daily recorded, and the time of death for each infected animal registered (Fig 1B).

Bottom Line: They possess the smallest genomes for a virulent or an attenuated ASFV, and are essentially identical except for a relatively small number of changes.We discuss the possible contribution of these changes to virulence.Analysis of the BA71 sequence allowed us to identify new similarities among ASFV proteins, and with database proteins including two ASFV proteins that could function as a two-component signaling network.

View Article: PubMed Central - PubMed

Affiliation: Centro Nacional de Microbiología, Instituto Nacional de Salud Carlos III, Majadahonda, Madrid, Spain.

ABSTRACT
The strain BA71V has played a key role in African swine fever virus (ASFV) research. It was the first genome sequenced, and remains the only genome completely determined. A large part of the studies on the function of ASFV genes, viral transcription, replication, DNA repair and morphogenesis, has been performed using this model. This avirulent strain was obtained by adaptation to grow in Vero cells of the highly virulent BA71 strain. We report here the analysis of the genome sequence of BA71 in comparison with that of BA71V. They possess the smallest genomes for a virulent or an attenuated ASFV, and are essentially identical except for a relatively small number of changes. We discuss the possible contribution of these changes to virulence. Analysis of the BA71 sequence allowed us to identify new similarities among ASFV proteins, and with database proteins including two ASFV proteins that could function as a two-component signaling network.

Show MeSH
Related in: MedlinePlus